L-Cysteine ​​CAS 52-90-4 (H-Cys-OH) Mayeso 98.5~101.0% (Titration) Factory High Quality

L-Cysteine ​​​​ili ndi osachepera 98.5% ndipo osapitirira 101.0% ya L-Cysteine ​​​​(C3H7NO2S), yowerengedwa pa zouma.
Kufotokozera L-Cysteine ​​amapezeka ngati makhiristo oyera kapena ufa woyera wa crystalline.Iwo ali khalidwe fungo ndi kukoma kokoma.Imasungunuka m'madzi momasuka, ndipo imakhala yosasungunuka mu ethanol (99.5).Imasungunuka mu 1 mol/L hydrochloric acid TS.
Chizindikiritso Dziwani kuchuluka kwa mayamwidwe a infrared a L-Cysteine ​​monga momwe amayankhidwira mu njira ya potassium bromide disk pansi pa Infrared Spectrophotometry <2.25>, ndipo yerekezerani sipekitiramu ndi Reference Spectrum: ma spectra onse amawonetsa kulimba kofanana kwa mayamwidwe amtundu womwewo.
Kuzungulira kwa kuwala <2.49> [a]20D:+8.0 ~+10.0° (2 g kuwerengedwa pa zouma, 1 mol / L hydrochloric acid TS, 25mL, 100 mm).
pH <2.54> PH ya yankho lokonzedwa mwa kusungunula 1.25 g ya L-Cysteine ​​​​mu 50 ml ya madzi ndi 4.7 mpaka 5.7.
Kuyera (1) Kumveka bwino ndi mtundu wa yankho-Sungani 1.0 g ya L-Cysteine ​​​​mu 20 ml ya madzi: yankho ndilomveka komanso lopanda mtundu.
(2) Chloride <1.03>-Sungani 0.30 g ya L-Cysteine ​​mu 10mL ya diluted nitric acid (1 mu 4), onjezerani 10mL ya hydrogenperoxide (30), kutentha kwa mphindi 20 mumadzi osamba otentha, ozizira, kenaka yikani madzi kuti apange 50 ml.Yesani kugwiritsa ntchito yankho ili ngati yankho loyesa.Konzani njira yowongolera ndi 0.35 mL ya 0.01 mol/L hydrochloric acid VS (osapitirira 0.041%)
(3) Sulfate <1.14>-Sula 0.6 g ya L-Cysteine ​​mu 30 ml ya madzi ndi 3 ml ya dilute hydrochloric acid, ndi kuwonjezera madzi kupanga 50 mL.Yesani kugwiritsa ntchito yankho ili ngati yankho loyesa.Konzani njira yothetsera motere: Ku 0,35 mL ya 0.005 mol/L sulfuric acid VS onjezerani 3 mL ya dilute hydrochloric acid ndi madzi kuti mupange 50 ml.Konzani yankho loyeserera ndi njira yowongolera ndi 4 ml ya barium chloride TS, motsatana (osapitirira 0.028%).
(4) Ammonium <1.02> -Yesani mayeso ndi 0.25 g ya L-Cysteine, pogwiritsa ntchito distillation pansi pa kupanikizika kochepa.Konzani njira yowongolera ndi 5.0 mL ya Standard Ammonium Solution (osapitirira 0.02%).
(5) Heavy Metals <1.07>-Pitirizani ndi 1.0g ya L-Cysteine ​​​​molingana ndi Njira 4, ndikuchita mayeso.Konzani njira yothetsera vutoli ndi 1.0 mL ya Standard Lead Solution (osapitirira 10 ppm).
(6) Iron <1.10> -Konzani njira yothetsera mayesero ndi 1.0 g ya L-Cysteine ​​​​molingana ndi Njira 1, ndikuyesani molingana ndi Njira A. Konzani njira yothetsera vutoli ndi 1.0mL ya Standard Iron Solution (osapitirira 10 ppm ).
(7)Zinthu Zogwirizana - Sungunulani 0.10 g ya L-Cysteine ​​mu njira ya N-ethylmaleimide (1 mu 50) kuti mupange 10 mL ndendende, siyani kwa mphindi 30, ndikugwiritsa ntchito yankho ili ngati yankho lachitsanzo.Chitoliro 1 mL yachitsanzo, onjezerani madzi kuti mupange ndendende 10 ml, pipet 1 ml ya yankho, onjezerani madzi kuti mupange 50 ml ndendende, ndipo gwiritsani ntchito yankholi ngati njira yothetsera (1).Payokha, sungunulani 0,10 g wa L-cystine mu 0.5mol/L hydrochloric acid TS kuti mupange ndendende 20 ml.Pipeni 1mL ya yankho ili, onjezerani madzi kuti mupange 100 ml, ndipo gwiritsani ntchito yankholi ngati yankho lokhazikika (2).Chitani mayesowo ndi mayankho awa monga momwe akuwongolera pansi pa Thin-layer Chromatography <2.03>.Malo 10mL iliyonse ya yankho lachitsanzo ndi njira zokhazikika (1) ndi (2) pa mbale ya gel osakaniza a chromatography yopyapyala.Pangani mbaleyo ndi osakaniza a1-butanol, madzi, ndi acetic acid (100) (3:1:1) mpaka mtunda wa masentimita 10, ndipo yimitsani mbaleyo kwa mphindi 30 pa 809C. Thirani mbaleyo mofanana ndi yankho. wa ninhydrin mu osakaniza methanol ndi asidi asidi (97: 3) (1 mu 100), ndiyeno kutentha pa 80 ℃ kwa mphindi 10: malo anapezedwa chitsanzo yankho lolingana ndi malo anapezerapo njira muyezo. (2) siili yolimba kuposa malo omwe amachokera ku yankho lokhazikika (2).Komanso, madontho ena kusiyapo malo oyamba ndi madontho omwe tawatchula pamwambapa kuchokera ku yankho lachitsanzo sali okulirapo kuposa malo omwe adachokera panjira yokhazikika (1).
Kutaya pa Kuyanika <2.41> Osapitirira 0.50% (1 g, mu vacuum, phosphorous (V) okusayidi, maola atatu).
Zotsalira pakuyatsa <2.44> Osapitirira 0.1%(1 g).
Kuyesa Kuyeza molondola za 0.2 g L-Cysteine, ikani mu botolo loyimitsidwa, ndi kusungunula mu 20 ml ya madzi.Sungunulani 4 g wa ayodini wa potaziyamu mu njira iyi, nthawi yomweyo ikani m'madzi ozizira oundana, onjezerani 5 mL wa dilute hydrochloric acid ndi ndendende 25 ml ya 0.05 mol/L ayodini VS, siyani pamalo amdima kwa mphindi 20, ndiyeno titrate<2.50 >Kuchuluka kwa ayodini wokhala ndi 0.1 mol/L sodium thiosulfate VS (chizindikiro: wowuma TS).Pangani kutsimikiza kopanda kanthu pogwiritsa ntchito njira yomweyo.
ML iliyonse ya 0.05 mol/L ayodini VS＝12.12 mg ya C3H7NO2S
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