L-Glutamine CAS 56-85-9 (H-Gln-OH) Mayeso 99.0~101.0% Factory High Quality

Japan PharmacopoeiaMiyezo Yothandizira
L-Glutamine [56-85-9]
L-Glutamine, ikauma, imakhala ndi osachepera 99.0% komanso osapitirira 101.0% ya L-Glutamine (C5H10N2O3).
Kufotokozera L-Glutamine imapezeka ngati yoyera, makhiristo kapena ufa wa crystalline.Iwo ali pang'ono khalidwe kukoma
Imasungunuka momasuka mu formic acid, imasungunuka m'madzi, ndipo imakhala yosasungunuka mu ethanol (99.5)
Chizindikiritso Dziwani kuchuluka kwa mayamwidwe a infrared a L-Glutamine monga momwe amayankhidwira mu njira ya potassium bromide disk pansi pa Infrared Spectrophotometry <2.25>, ndikuyerekeza mawonekedwewo ndi Reference Spectrum: ma spectra onse amawonetsa kulimba kofanana kwa mayamwidwe amtundu womwewo.
Kasinthasintha wa Optical <2.49> [a]20D:+6.3° mpaka +7.3° Yesani molondola pafupifupi 2 g L-Glutamine, zouma kale, onjezerani 45mL madzi, otentha mpaka 40 ℃ kuti asungunuke, ndipo mutatha kuzirala, onjezerani madzi kuti mupange. kwenikweni 50 ml.Dziwani kusinthasintha kwa njira iyi mu cell ya 100 mm, mkati mwa mphindi 60.
pH <2.54> PH ya yankho lokonzedwa mwa kusungunula 1.0 g ya L-Glutamine mu 50 mL ya madzi ndi pakati pa 4.5 ndi 6.0.
Chiyero
(1) Kumveka bwino ndi mtundu wa yankho - Yankho lopezedwa mwa kusungunula 0.5 g ya L-Glutamine mu 20 ml ya madzi ndi omveka komanso opanda mtundu.
(2) Chloride <1.03> -Yesani mayeso ndi 0.5 g ya L-Glutamine.Konzani njira yowongolera ndi 0.30 mL ya 0.01 mol/L hydrochloric acid VS (osapitirira 0.021%).
(3) Sulfate <1.14> -Chitani mayeso ndi 0.6 g ya L-Glutamine.Konzani njira yowongolera ndi 0.35 mL ya 0.005 mol/L sulfuric acid VS (osapitirira 0.028z).
(4) Ammonium <1.02>—Yesani mayeso ndi 0.10 g ya L-Glutamine, pogwiritsa ntchito distillation pansi pa kupanikizika kochepa.Konzani njira yowongolera ndi 10.0 mL ya Standard Ammonium Solution.Kutentha kwa madzi osamba ndi 45 ℃ (osapitirira 0.1%)
(5) Zitsulo Zolemera <1.07> -Pitirizani ndi 1.0 g ya L-Glutamine molingana ndi Njira 1, ndikuyesa mayeso.Konzani njira yowongolera ndi 1.0 mL ya Standard Lead Solution (osapitirira 10 ppm).
(6) Iron <1.10>-Konzani njira yothetsera mayesero ndi 1.0 g ya L-Glutamine molingana ndi Njira 1, ndikuyesani molingana ndi Njira A. Konzani njira yothetsera vutoli ndi 1.0 mL ya Standard Iron Solution (osapitirira 10 ppm)
(7) Zinthu Zogwirizana - Sungunulani 0,10 g ya L-Glutaminein 10 mL ya madzi, ndipo gwiritsani ntchito yankho ili ngati yankho lachitsanzo.Chitoliro 1 mL yachitsanzo, onjezerani madzi kuti mupange 10 ml ndendende.Pipeni 1 ml ya yankho ili, onjezerani madzi kuti mupange ndendende 50 ml, ndipo gwiritsani ntchito yankho ili ngati yankho lokhazikika.Chitani mayeso ndi mayankho awa monga momwe akuwongolera pansi pa Thin-layer Chromatography <2.03>.Malo 5mL iliyonse ya yankho lachitsanzo ndi yankho lokhazikika pa mbale ya silicagel ya chromatography yopyapyala.Kenako pangani zosakaniza za 1-butanol, madzi ndi acetic acid (100) (3:1:1) mpaka mtunda wa 10 cm, ndikuwumitsa mbaleyo pa 80 ℃ kwa mphindi 30.Sayani molingana ndi yankho la ninhydrin mu osakaniza methanol ndi asidi asidi (100) (97:3) (1 mu 100) pa mbale, ndi kutentha pa 80 ℃ kwa mphindi 10: malo ena osati malo oyamba opezeka ndi yankho lachitsanzo silili lamphamvu kwambiri kuposa malo omwe amapezeka ndi yankho lokhazikika.
Kutaya pa Kuyanika <2.41> Osapitirira 0.3% (1 g, 105 ℃, maola 3)
Zotsalira pakuyatsa <2.44> Osapitirira 0.10%(1 g).
Kuyesa Kuyeza molondola za 0.15 g ya L-Glutamine, yowumitsidwa kale, sungunuka mu 3 mL ya formic acid, kuwonjezera 50 mL ya acetic acid (100), ndi titrate <2.50> ndi 0.1 mol/L perchloric acid VS (potentiometric titration).Chitani chitsimikiziro chopanda kanthu mwanjira yomweyo, ndipo pangani kukonza kulikonse kofunikira.
ML iliyonse ya 0.1 mol/L perchloric acid VS＝14.61 mg ya C5H10N2O3
Zotengera ndi Zosungirako—Zotengera zolimba.

USP Reference Standards <11> -USP Glutamine RS.
Chizindikiritso, Mayamwidwe a Infrared <197K>.
Kuzungulira Kwachindunji <781S>: pakati pa +6.3 ° ndi +7.3 °, yotsimikizika pa 20 ℃
Test Solution- Mu botolo loyenera, konzani yankho la pafupifupi 40mg pa ml m'madzi pafupifupi 40 ℃.Kuziziritsa ndi kuchepetsa ndi madzi kuti voliyumu musanagwiritse ntchito.
Kutaya pa Kuyanika <731>-Yanikani pa 105 ℃ kwa maola 3;imataya zosaposa 0,20% ya kulemera kwake.
Zotsalira pa Ignition <281>Osapitirira 0.30%
Chloride <221>-Gawo la 0.7-g limasonyeza kuti palibe kloride yochulukirapo kuposa 0.50ml ya 0.020 N hydrochloric acid: osapitirira 0.05% amapezeka.
Sulfate <221>-Gawo la 0.8-g limasonyeza kuti palibe sulfate yochulukirapo kuposa 0.25ml ya 0.020 N sulfuric acid: osapitirira 0.03% amapezeka.
Chromatographic Purity
Adsorbent: 0.25-mm wosanjikiza wa chromatographic silica gel osakaniza.
Njira Yoyesera: 10mg pa ml, m'madzi
Standard Solution-Konzani yankho la USP Glutamine RS m'madzi okhala ndi ndende yodziwika pafupifupi 0.05mg pa ml.
Kuchuluka kwa ntchito: 5μL
Kupanga zosungunulira System: chisakanizo cha mowa wa butyl, madzi, ndi glacial acetic acid (3:1:1).
Spray Reagent-Sungani 0.2g ya ninhydrin mu 100ml wosakaniza wa butyl mowa ndi 2 N acetic acid (95:5)
Njira-Chitani monga momwe mwalangizira chromatography yocheperako pansi pa Chromatography <621>, kenako yimitsani mbale pa 80 ℃ kwa mphindi 30.Uza mbaleyo ndi reagent yopopera, kutentha pa 80 ℃ kwa mphindi 10, ndikuwunika pansi pa kuwala koyera: palibe malo achiwiri mu chromatogram yotengedwa kuchokera ku yankho la mayeso omwe ali okulirapo kapena olimba kuposa malo oyambira mu chromatogram yopezedwa kuchokera ku yankho la standrad. (0.5%)
Organic Volatile Zonyansa, Njira I <467>: Kukwaniritsa zofunikira.
Asssy- Tumizani pafupifupi 150mg ya Glutamine, yolemedwa bwino, ku botolo la 125ml, kusungunula mu 3ml ya formic acid ndi 50ml ya glacial acetic acid, ndi titrate ndi 0.1 N perchloric acid VS kutsimikizira mapeto potentiometrically.Chitani chitsimikiziro chopanda kanthu, ndipo pangani kuwongolera kulikonse kofunikira (onani Titrimetry<541>).ml iliyonse ya 0.1 N perchloric acid ndi yofanana ndi 14.615 mg ya C5H10N2O3