L-Histidine CAS 71-00-1 (H-His-OH) Mayeso 98.5~101.0% Factory High Quality

L-Histidine (H-His-OH) (CAS: 71-00-1) Njira Yoyesera ya AJI97
Chizindikiritso: Fananizani kuchuluka kwa mayamwidwe a infrared a chitsanzo ndi njira ya potaziyamu bromide disc.
Kuzungulira Kwapadera [α]20/D: Zitsanzo Zouma, C=11, 6MOL/L HCl
State of Solution (Transmittance): .5g mu 10ml wa H2O, sungunulani ndi kutentha spectrophotometer, 430nm, 10nm selo makulidwe.
Chloride (Cl): 0.7g, A-1, ref: 0.40ml ya 0.01mol/L HCl
Sulfate (SO4): 1.2g, (1), ref: 0.50ml ya 0.005mol/L H2SO4
Chitsulo (Fe): 1.5g, (1), ref: 1.5ml ya Iron Std.(0.01mg/ml)
Zitsulo Zolemera (Pb): 2.0g, (1), pH = 7, ref: 2.0ml ya Pb Std.(0.01mg/ml)
Arsenic (As2O3): 2.0g, (1), ref: 2.0ml ya As2O3 Std.
Ma Amino Acid Ena: Zitsanzo Zoyesera: 50μg, B-6-a, kulamulira: L-His 0.25μg
Kutaya pa Kuyanika: pa 105 ℃ kwa maola atatu.
Kuyesa: Zitsanzo zouma, 150mg, (1), 2ml ya formic acid, 50ml ya glacial acetic acid, 0.1mol/L HCLO4 1ml = 15.516mg C6H9N3O2
Mayeso a pH: 1.0g mu 50ml ya H2O)

L-Histidine (H-His-OH) (CAS: 71-00-1) USP36-NF31 Njira Yoyesera
TANTHAUZO
Histidine ili ndi NLT 98.5% ndi NMT 101.5% ya L-Histidine (C6H9N3O2), yowerengedwa pa maziko owuma.
CHIZINDIKIRO
A. INFRARED ABSORPTION <197K> Zitsanzo ndi USP L-Histidine RS zidapangidwanso kuchokera ku 80% mowa.
ZOYESA
NTCHITO
Chitsanzo: 150 mg ya Histidine
Chopanda kanthu: Sakanizani 3 ml ya formic acid ndi 50 ml ya glacial acetic acid.
Titrimetric system
(Onani Titrimetry <541>)
Mode: Direct titration
Titrant: 0.1 N perchloric acid VS
Kuzindikira komaliza: Potentiometric
Kusanthula: Sungunulani Chitsanzo mu 3 ml ya formic acid ndi 50mL ya glacial acetic acid.Titrate pang'onopang'ono ndi Titrant.Chitani kutsimikiza kopanda kanthu.
Kuwerengera kuchuluka kwa Histidine (C 6H9N3O2) mu Zitsanzo zomwe zatengedwa:
Zotsatira = {[(VS-VB) x N x F]/W} x100
VS= Voliyumu ya Titrant yogwiritsidwa ntchito ndi Zitsanzo (mL)
VB = Voliyumu ya Titrant yodyedwa ndi Chopanda kanthu (mL)
N = chizolowezi chenicheni cha Titrant (mEq/mL)
F= chinthu chofanana, 155.2 mg/mEq
W = kulemera kwachitsanzo (mg)
Njira zolandirira: 98.5% ~ 101.5% pazowuma
ZOCHITSA
ZOKHALA PA KUYATSA <281>: NMT 0.4%
CHLORIDE NDI SULFATE, Chloride <221>
Njira yothetsera: 0.50mL ya 0.020 N hydrochloric acid
Chitsanzo: 0.73g ya Histidine
Njira zolandirira: NMT 0.05%
CHLORIDE NDI SULFATE, Sulfate <221>
Njira yothetsera: 0.10mL ya 0.020 N sulfuric acid
Chitsanzo: 0.33g ya Histidine
Njira zolandirira: NMT 0.03%
IRON <241>: NMT 30ppm
Chotsani zotsatirazi:
ZINTHU ZONSE, Njira I <231>: NMT 15ppm
ZOKHUDZANA NDI COMPOUNDS
Njira yothetsera kuyenerera: 0.4mg/mL iliyonse ya USP L-Histidine RS ndi USP L-Proline RS
Njira yothetsera: 0.05mg/mL ya USP L-Histidine RS m'madzi.[Dziwani-Yankho ili lili ndi ndende yofanana ndi 0.5% ya Sample solution.]
Yankho lachitsanzo: 10mg/mL ya Histidine m'madzi
Chromatographic system (Onani Chromatography <621>, Thin-Layer Chromatography.)
Njira: TLC
Adsorbent: 0.25-mm wosanjikiza wa chromatographic silica gel osakaniza
Kuchuluka kwa ntchito: 5μL
Kupanga zosungunulira: Mowa wa Butyl, glacial acetic acid, ndi madzi (3:1:1)
Utsi wopopera: 2 mg/mL wa ninhydrin wosakaniza wa mowa wa butyl ndi 2N acetic acid (95:5)
Kuyenerera kwadongosolo
Zofunika pazabwino: Chromatogram ya njira yothetsera kukwanira kwa System imawonetsa mawanga awiri olekanitsidwa bwino.
Kusanthula
Zitsanzo: Njira yothetsera kuyenerera kwadongosolo, yankho lokhazikika, ndi yankho lachitsanzo.
Mukayanika mbaleyo, pukutani ndi Spray reagent, ndi kutentha pakati pa 100 ° ndi 105 ° kwa mphindi 15.Yang'anani mbaleyo pansi pa kuwala koyera.
Njira zolandirira: Malo aliwonse achiwiri a Sample solution si aakulu kapena okulirapo kuposa malo oyambira a Standard solution.
Zonyansa zapayekha: NMT 0.5%
Zonyansa zonse: NMT 2.0%
MAYESO AKE
KUSINTHA KWA MASO, Kuzungulira Kwachindunji <781S>
Yankho lachitsanzo: 110 mg/mL mu 6 N hydrochloric acid
Njira zolandirira: +12.6 ° mpaka +14.0 °
PH <791>
Yankho lachitsanzo: 20 mg/mL yankho
Zovomerezeka zovomerezeka: 7.0~8.5
LOSSON PAKUYANTHA <731>: Yanikani chitsanzo pa 105℃ kwa 3 h: imataya NMT 0.2% ya kulemera kwake.
ZOWONJEZERA ZOFUNIKA
KUPAKA NDI KUSEKA: Sungani m’zotengera zotsekedwa bwino.
MFUNDO ZA USP REFERENCE <11>
USP L-Histidine RS
USP L-Proline RS