Phthalimide Potassium Salt CAS 1074-82-4 Chiyero >99.0% (HPLC)

3. Njira yoyesera
3.1 Kutaya pakuyanika (W/W)
3.1.1 Zida ndi zida
Chimodzi chopitilira 10,000 zamagetsi zamagetsi, ng'anjo yowumitsa yamagetsi yamagetsi, 40 * 25 botolo lolemera, chowumitsira
Reagent: silika gel
3.1.2 Njira
1. Tsukani mabotolo oyezera 3 40*25.
2, ikani mu uvuni pa 105 ℃ (± 2 ℃) youma kwa ola limodzi.
3. Tulutsani botolo la 40 * 25 ndikuyiyika mu chowumitsira kuti muzizizira kwa 30min.
4, Zolondola zolemera 40 * 25 botolo loyezera lopanda kanthu W0.
5. Gwiritsani ntchito botolo la 40 * 25 kuti muyese sampuli yosakanikirana yofanana ya 3.0000g (W1), okwana 3.
6, Ikani mu uvuni, chotsani kapu ya botolo, ikani pafupi ndi botolo loyezera, kapena theka tsegulani chipewa cha botolo kuti muwume, chotsani, chikhale cholemera bwino cha botolo, chouma pa 105 ℃ (± 2 ℃) 3 maola.
7. Tulutsani botolo la 40 * 25 ndikuyiyika mu chipangizo chowumitsa kuti muzizizira kwa 30min.
8, Kulemera kolondola mutatha kuyanika 40 * 25 kulemera kwa botolo W2.
Kuwerengera: Kutaya pakuyanika (%) =(W1-W2)/(W1-W0)×100%
3. 4 Kutsimikiza kokhutira
3.4.1 Zida ndi zida
LC-UV100 Liquid Chromatograph (Shanghai Wufeng Scientific Instrument Co., LTD.)
10ml botolo la volumetric, supuni yakona yachitsulo chosapanga dzimbiri ndi zina zofananira
3.3.2 Njira
Njira yowunikira mchere wa Phthalimide Potassium
3.3.3 Chromatography yamadzimadzi
Chromatographic chikhalidwe
Mzere wa Chromatographic: HypersilC6H5 (C8 ikupezekanso) 4.6 * 250mm 5um
Kutalika kwa mawonekedwe: 210nm
Kutentha kwapakati: 30 ℃
Kuthamanga: 1.0ml / min
Zitsanzo za ndende: 0.05mg/ml (diluent ndi gawo la mafoni)
Ma reagents
Potaziyamu Phosphate Dibasic (analytical pure)
Phthalimide Potassium Salt (chitsanzo chokhazikika)
Acetonitrile (analytical pure)
Madzi oyera
Gawo lam'manja: acetonitrile: phosphate buffer (PH:3.5) =40:60
Kulemera 1.63g Potaziyamu Phosphate Dibasic ndi kupasuka mu 600ml madzi oyera, kusintha PH mtengo 3.5 ndi phosphoric acid, kutsanulira mu 1000ml beaker munali 400ml acetonitrile, sakanizani bwino, fyuluta ndi 0,45um wosanganiza CHIKWANGWANI fyuluta nembanemba, kupasuka ndi akupanga fyuluta nembanemba. ntchito.
3.3.4 Ndondomeko
1) Kukonzekera kuyesa njira (0.05mg/ml)
Chitsanzo cha 2.5mg chinayesedwa molondola ndikuyika mu botolo la volumetric 50ml.The mafoni gawo anawonjezera ndi kuchepetsedwa kuti lonse ndi akupanga kuvunda.
2) kasinthidwe muyezo njira (1.0mg/ml)
Chitsanzo chodziwika bwino cha 2.5mg chinayesedwa molondola ndikuyika mu botolo la 50ml volumetric.The mafoni gawo anawonjezera ndi kuchepetsedwa kuti lonse ndi akupanga kuvunda.
3) Kugwiritsa ntchito dongosolo:
(1) Ikani mutu wa fyuluta mu 80% methanol poyambira, tsegulani valavu yotulutsa mpweya ndi kukhetsa kwa 3-5min, ndi kutseka valavu yotulutsa mpweya ndikutsuka kwa 10-15min.Kenako ikani mutu wa fyuluta mu 90% madzi oyera, tsegulani valavu yokhetsa ndikukhetsa kwa 3-5min, kutseka valavu yokhetsa ndikutsuka kwa 10min.
(2) Kenako sinthani gawo lokonzekera la m'manja, kuchuluka kwa otaya ndi 1.0ml/mphindi, kukakamiza kutsogolo ndi kochepera 1000Psi, tsegulani chojambulira, dikirani mpaka kupanikizika kutsogolo kukhazikika, tsegulani makina ogwiritsira ntchito makompyuta, ndikulowa pa intaneti.
(3) Pambuyo pa mzati kutsogolo kukhazikika, tsegulani chowunikira.Choyamba bayani singano imodzi ya gawo la mafoni, kenaka bayani singano imodzi yachitsanzo chokhazikika, kenako bayani chitsanzo.
(4) Kuwerengera zotsatira: dera normalization njira
(5) Mukamaliza ntchito yowunikira, tsegulani chowunikira choyamba, ndiyeno mutsuka mutu wa fyuluta m'madzi oyera kwa 10-15min;Nthawi yomweyo, valavu ya jakisoni idatsukidwa ndi madzi ndi methanol.
(6) Pomaliza, valani 80% methanol, muzimutsuka kwa 30min, ndikutseka.
4. Mukamaliza kuyendera, lembani pepala loyesera ndi labotale.