L-Glutamic Acid α-Benzyl Ester CAS 13030-09-6 (H-Glu-OBzl) Su'ega >98.5% (HPLC) Tulaga Maualuga

1. Mama (HPLC)
1.1 Meafaigaluega
Ole chromatography vai maualuga, UV3000 detector.
Paleni au'ili'ili faaeletonika
1.2 Reagents
Acetonitrile (vaega chromatographic), TFA (vaega chromatographic)
1.3 Tulaga Chromatographic
1.3.1 Koluma: koluma C18, 5 μl * 250 * 4.6 mm
1.3.2 Sailiga umi galu: 220nm
Fuafuaga tafe: 1.0 mL/min
Volume tui: 10 μL (mo faʻamatalaga)
Fa'asusu: acetonitrile
Taimi ta'avale: 20 minute
1.4 Sauniuniga vaega feaveai
Vaega feavea'i A (0.1% Trifluoroacetic acid Suavai): sa'o mitiia 1.0 ml TFA diluted i le 1000 ml i le vai, fefiloi ma degas.
Vaega feaveaʻi B (0.1% Trifluoroacetic acid Acetonitrile): faʻafefe saʻo le 1.0 ml TFA, faʻafefe i le 1000 ml ma le acetonitrile, faʻafefiloi ma degas.
1.5 Polokalama Gradient
Taimi (min) A% B%
0:00 90 10
10.00 10 90
15.00 10 90
15.01 90 10
20.00 90 10
1.6 Sauniuniga o fofo faataitai
Fua le 0.1 g faʻataʻitaʻiga ma faʻamavae i le acetonitrile ma faʻafefe i le 100ml.Lulu lelei mo le faʻaaogaina i le lumanaʻi, poʻo le faʻaoga tutusa.Saunia ni faataitaiga se lua e tutusa.
1.7 Fa'ata'ita'iga Filifiliga
Iloilo le fa'ata'ita'iga e tusa ai ma faiga fa'ata'ita'iga nei:
1) E sili atu i le 1 tui ole fofo avanoa
2) 1 tui ole fofo faataitai 1#
3) 1 tui ole fofo faataitai 2#
1.8.0 Fa'atatau
1.8.1 O le pito i luga o le eria normalization metotia na faʻaaogaina e fuafua ai le mama o le HPLC.
1.8.2 Ole eseesega ole fa'atatau ole mama ole nila e lua e le tatau ona sili atu ile 1%
1.8.3 Afai o taunuuga o tui uma e lua e fetaui ma tulaga taliaina, ave le averesi mama o le taunuuga mulimuli.