D-Phenylalanine CAS 673-06-3 (HD-Phe-OH) Su'ega 98.0~102.0% Falegaosimea 50MT/Masina

1. Fa'aaliga-- Su'ega va'aia
2. Ta'amilosaga fa'apitoa-- E fua fa'atatau i le GB/T613-1988.
Sauniuniga faʻataʻitaʻiga: Fua saʻo le 0.5000g faʻataʻitaʻiga ma faʻanofo i se fagu volumetric mama ma mago 50ml, faʻaopopo le 20ml vai, ufiufi le fagu, lulu e faʻamavae, ona faʻafefe ai lea i le fua i le vai.
Su'ega: Fetuuna'i le zero o le gyroscope a'o le'i faia le su'ega, ona uta lea o le paipa su'ega ma le fa'ata'ita'iga vaifofo, fa'amaumau le Angle rotation, ma fuafua le suiga patino o le fa'ata'ita'iga ma le fua fa'atatau.
[a]D20 = (r×50) ÷(L×W)
O fea:
[a]D20: Va'aiga fa'apitoa fa'apitoa ile 20℃ ole fa'ata'ita'iga fofo
r: O le suiga o le mata ua matauina i le 20 ℃ mo le faʻataʻitaʻiga fofo
50: Volume o fofo fa'ata'ita'i saunia (ml)
w: Fa'ata'ita'iga mamafa (g)
L: Umi o paipa fe'avea'i mata'utia (dm)
3. Mu o totoe
Ave pe tusa ma le 1g o lenei oloa ma faʻavevela i le 600 ℃, faʻavevela seia oʻo ina lefulefu atoa e tusa ai ma le faʻatonuga o le Faʻaopoopoga Ⅷ N o le Vaega II o le CP2010 Edition, faʻaopopo le 0.5-1.0ml concentrated sulfuric acid ma vevela (700℃-). 800℃) i le mamafa faifaipea.
3.1 "Toega Incandescent" i lenei Metotia (Chinese Pharmacopoeia 2005 Lomiga, Vaega II Faʻaopoopo Ⅷ N) e faasino i le oxide uʻamea poʻo sulfates totoe pe a maeʻa vailaʻau (tele o mea faʻapipiʻi) e faʻavevela seia oʻo i le efuefu atoatoa, ona faʻaopoopoina lea o le sulfuric acid 0.5 ~ 1.0ml ma incandesced (700 ~ 800 ℃) i le mamafa faifai pea.
4 Chiral mama (HPLC)
Meafaigaluega: chromatography vai maualuga, su'esu'e PDA.paleni su'esu'e faaeletonika, reagent;Tulaga ole chromatography
Tulaga o le Chromatographic: Column: Astec CHIROBIOTICTMT HPLC Column 250X4.6mm, 5ul, le umi o le galu, fua o le tafe, tele faʻataʻitaʻiga:10 u L(faʻasinomaga) manifinifi: vai, taimi aoina o faʻamatalaga:20.00min.
Fa'ata'ita'iga fa'ata'ita'iga: Sa su'esu'eina le fa'ata'ita'iga e tusa ai ma le fa'ata'ita'iga fa'ata'ita'iga:1 nila o le vaifofo avanoa ma le 1 nila ole DL-phenylalanine fa'asinomaga.
1 tui fa'ata'ita'iga fofo,
Fa'ai'uga Fa'atatau: O mea o lo'o i totonu o le HPLC na fa'amautu e la'ititi ifo i le 0.5% e tusa ai ma le fa'avasegaina o vaega pito i luga ma le toesega avanoa.
5. Mago ma leai se mamafa
5.1 Meafaigaluega:
ogaumu fa'amago fa'amago, 1/10,000 paleni.
5.2 Taualumaga:
I totonu o se fagu mafolafola mamafa ma le mamafa faifaipea ma le fa'amamago le ufiufi gutu o le eleele, fua le 1 kalama (sa'o i le 0.0001 kalama) o le fa'ata'ita'iga.O le faʻataʻitaʻiga e tatau ona faʻasalalau solo i le pito i lalo o le fagu mamafa ma le mafiafia e le sili atu i le 10mm, tuʻu i totonu o se ogaumu faʻamago eletise eletise, faʻamago i le 105-110 ℃ mo le 3 itula, ona alu lea i totonu o le potu faʻagogo e malulu i vevela potu mo le fuaina.
Fuafuaga: Pa'u mamafa mago %= (M1-M2) ÷M×100
O fea: M1: tele o fa'ata'ita'iga ma fagu fua a'o le'i fa'amago, kalama
M2: Massa o faʻataʻitaʻiga ma fagu fua pe a uma ona faʻamago, i le kalama
M: Fa'ata'ita'i mamafa, kalama
Laasaga 6: Anotusi
6.1 Meafaigaluega
Ole chromatography vai maualuga, su'esu'e PDA.
Paleni au'ili'ili faaeletonika
6.2 Reagents
Acetonitrile (vaega chromatographic), TFA (vaega chromatographic)
6.3 Tulaga Chromatographic
6.3.1 Koluma: YMC-ODS-AM, 5 μL, 150x4.6 mm
6.3.2 Sailiga umi galu: INC220nm
Fuafuaga tafe:1.0mL/min
Tele faʻataʻitaʻiga: 10μL (mo faʻamatalaga)
Mea manifinifi: Acetonitrile
Taimi e aoina ai fa'amaumauga: 20.00min
6.4 Sauniuniga vaega feaveai
Vaega feavea'i A (0.1% trifluoroacetic acid vai): sa'o absorption 2. Dilute Oml trifluoroacetic acid i le vai i le 2000m1, faʻafefiloi lelei, ma degas;
Vaega feavea'i B(0.1% acetonitrile trifluoroacetic acid): sa'o absorption 2.Oml trifluoroacetic acid sa diluted i 2000m1 ma acetonitrile, fefiloi ma deggated;
6.5 Polokalama fa'asolosolo vaega fe'avea'i
Taimi (min) A% B%
0.00 90 10
12.00 10 90
15.00 10 90
15.01 90 10
20.00 90 10
6.6 Sauniuniga o fofo faataitai
Fua ma fa'amuta 0.1g fa'ata'ita'iga fa'atasi ai ma le acetonitrile, fa'afefete i le 100m1, lulu lelei mo le fa'aoga, po'o le fa'aoga tutusa.Saunia ni faataitaiga se lua e tutusa.
6.7 Fa'ata'ita'iga Filifiliga
Iloilo le fa'ata'ita'iga e tusa ai ma faiga fa'ata'ita'iga nei:
E silia ma le 1 tui ole vaifofo avanoa
1 nila fa'ata'ita'iga fofo 1#
1 nila fa'ata'ita'iga fofo 2#
6.8 I'uga Fa'atatau
2.8.1 Na fa'aogaina le fa'atama'ia o le vaega pito i luga e fa'atatau i le mama o le HPLC e ala i le toese o avanoa avanoa.
2.8.2 Ole eseesega ole fa'atatau ole mama ole nila e lua e le tatau ona sili atu ile 1%
2.8.3 Afai o taunuʻuga o tui e lua e fetaui ma tulaga taliaina, o le averesi o le mama e avea ma taunuuga mulimuli.