EDC·HCl CAS 25952-53-8 Feso'ota'i Reagent Mama >99.0% (T) Falegaosimea

1-(3-Dimethylaminopropyl) -3-Ethylcarbodiimide Hydrochloride (EDC · HCl) (CAS: 25952-53-8) o se paʻepaʻe tioata paʻepaʻe, faigofie deliquescent, soluble i le vai, soluble i ethanol, faʻaaogaina e avea o se faʻagaioiga faʻamalosi mo carboxyl. vaega i amide synthesis, faʻaaogaina foʻi mo le faʻagaoioia o vaega phosphate, fesoʻotaʻiga o porotini ma nucleic acids, ma le gaosiga o immunocouplers.EDC-HCl o loʻo faʻaaogaina i le pH o le 4.0-6.0 ma e masani ona faʻaogaina faʻatasi ma le N-Hydroxysuccinimide (NHS) poʻo le N-Hydroxysulfosuccinimide sodium masima e faʻaleleia ai le lelei o le faʻaogaina.
EDC · HCl o le carbodiimide e mafai ona solu vai, faʻaaogaina lautele mo le faʻapipiʻiina o peptide.EDC · HCl o loʻo faʻaaogaina e avea o se mea e faʻamalosia ai le carboxyl.E iai sona sao taua mo le le mafaia o biomolecules tetele e fesoʻotaʻi ma le N-hydroxysuccinimide.E faʻaaogaina foi i le acylation o phosphoranes.
Suavai-soluble derivative o carbodiimide aoga mo le conjugating haptens i polotini ma polypeptides.Faʻaaogaina e faʻafeiloaʻi ai le NMDA ma avea o se mea faʻapipiʻi i le peptide synthesis.O le aoga tele o le fa'aogaina o le EDAC o le faigofie ona aveese o le sili atu o le reagent ma le urea tutusa e ala i le fufuluina i le suka fa'afefe po'o le vai.Carbodiimides e faʻamalosia le faʻavaeina o fusi amide, carboxylic acids, ma amines e ala i le faʻagaoioia o le carboxylate e fausia ai se O-acylurea.O lenei mea vavalo e mafai ona osofaʻia e se amine saʻo e fai ai se amide.O le EDAC o lo'o fa'asa'olotoina e avea o se soluble urea derivative.
EDC·HCl e mafai ona faʻaogaina mo le faʻapipiʻiina o amides.E faʻaaogaina e fai ma sui soʻotaga i le faʻapipiʻiina o esters mai acids carboxylic e faʻaaoga ai le dimethylaminopyridine e fai ma faʻamalosi.
E masani ona tolu faʻaaogaina faʻamaʻi faʻapipiʻi: DCC, DIC ma EDC · HCl.O le faʻaogaina o lenei ituaiga o condensing agent e masani ona manaʻomia le faʻaopoopoga o faʻamaʻi acylation poʻo activators, e pei o le DMAP, HOBt, ma isi. e le o faaaogaina catalyst e faaliliu ai i le ester galue tutusa po o le amide galue, o le a toe faatulaga i totonu o le fale mautu o le urea (Ala B).Condensation activator: E i ai ituaiga nei o condensation activator masani ona faaaoga.I le taimi nei, 4-N, N-dimethylpyridine (DMAP) ua faʻaaogaina lautele e faʻapipiʻi ai faʻalavelave acylation eseese.O nisi taimi e mafai ona faʻaaogaina le 4-PPY pe a le lelei le aʻafiaga o le DMAP.E tusa ai ma tusitusiga talafeagai, o lona malosi faʻamalosi e tusa ma le afe taimi maualuga atu nai lo le DMAP.i totonu o condenser masani e tolu, DCC ma DIC e taugofie.E masani ona fa'aoga fa'atasi le DCC ma le DMAP.O se tasi o le sili ona le lelei o le faʻaaogaina o le DCC o le isi oloa o le tali, dicyclohexylurea, o loʻo i ai se solubility laʻititi i le vaega faʻale-aganuʻu lautele ae e fai si soluble, o lea e faigata ai ona aveese maeʻaeʻa e ala i nisi o auala masani faʻamamaina, recrystallization, chromatography koluma, ma isi;ona o le solubility o le dicyclohexylurea i le ether e fai si laʻititi nai lo isi solvents, o le mea lea, o le togafitiga o lenei ituaiga o tali e masani lava ona faʻafefe le solvent tali ma faʻaopoopo le ether, ma faʻamama ese le tele o le dicyclohexylurea aʻo leʻi faia nisi togafitiga.Talu ai ona o le diisopropylurea na gaosia e le DIC e sili atu le solubility i solu faʻaola lautele, e masani ona faʻaaogaina i le kemisi tuʻufaʻatasia solid-vase synthesis.O le EDCI o loʻo faʻaaogaina nei i le tele o vailaʻau kemisi.O se tasi o ona uiga autu o le urea e gaosia pe a uma le tali e solu vai ma faigofie ona fufulu ese.E masani lava, o le EDCI e tu'ufa'atasia ma le HOBt (fa'aaliga: o lenei tali HOBt e masani ona mana'omia, a le o lea e ono o'o atu ai i le maualalo o le fa'aputuga o fua).O nisi taimi pe afai o le-tulaga faalavefau o le acid e tele pe o loʻo i ai se vaega faʻaeletonika aveese, o le tali o le a tumau i le laasaga ester galue (o le ester malosi o loʻo i ai se faailoilo tele alaleo malosi ma e mafai ona iloa e MS poʻo LC-MS).