Fmoc-Ser(tBu)-OH CAS 71989-33-8 Fmoc-O-tert-Butyl-L-Serine Purity >98.5% (HPLC)

Fa'asologa o asiasiga
1. Folafolaga
Su'ega va'aia
2. Mama (HPLC)
2.1 Meafaigaluega
Ole chromatography vai maualuga, su'esu'e PDA.
Paleni au'ili'ili faaeletonika
2.2 Reagent
Acetonitrile (vaega chromatographic), acid trifluoroacetic (vaega chromatographic)
2.3 Tulaga Chromatographic
2.3.1 Koluma: YMC-ODS-AM, 5 μl, 250x4.6 mm
2.3.2 Sailiga umi galu: INC220nm
Fuafuaga tafe: 1.0mL/min
Fa'ata'ita'iga tele:10 μl (fa'asino)
Fa'asusu: acetonitrile
Taimi e aoina ai fa'amaumauga: 25.00min
2.4 Sauniuniga vaega feaveai
Vaega feavea'i A (0.1% trifluoroacetic acid vai): sa'o absorption 2. Dilute Oml trifluoroacetic acid i le vai i le 2000m1, faʻafefiloi lelei, ma faʻamalo;
Vaega feavea'i B (0.1% acetonitrile trifluoroacetic acid): sa'o absorption 2.0ml trifluoroacetic acid sa fa'afefeteina i le 2000m1 ma le acetonitrile, fefiloi ma degassing;
2.5 Polokalama fa'alili ole vaega feavea'i
Taimi (min) A% B%
0.00 90 10
13.00 10 90
18.00 10 90
18.01 90 10
23.00 90 10
2.6 Sauniuniga o fofo faataitai
Fua ma fa'amuta 0.1g fa'ata'ita'iga fa'atasi ai ma le acetonitrile ma fa'afefete i le 100ml, lulu lelei mo le fa'aoga, po'o le fa'atonuga tutusa.Saunia ni faataitaiga se lua e tutusa.
2.7 Fa'ata'ita'iga Filifiliga
Iloilo le fa'ata'ita'iga e tusa ai ma faiga fa'ata'ita'iga nei:
E silia ma le 1 tui ole vaifofo avanoa
1 su'i fa'ata'ita'iga fofo 1#
1 su'i fa'ata'ita'iga fofo 2#
2.8 Fa'atatauga o taunu'uga
2.8.1 Na fa'aogaina le fa'atama'ia o le vaega pito i luga e fa'atatau i le mama o le HPLC e ala i le toese o avanoa avanoa.
2.8.2 Ole fua faatatau ole eseesega ole mama o nila e lua e le tatau ona sili atu ile 1%
2.8.3 Afai o taunuʻuga o tui e lua e fetaui ma tulaga taliaina, o le averesi o le mama e avea ma taunuuga mulimuli.
3, Mea liusuavai -- RY-1 mea fa'ameamea
4. leiloa i luga o le fa'amago metotia su'esu'e
4.1 Meafaigaluega:
Omea fa'amago eletise eletise, tasi i le sefulu afe paleni.
4.2 Taualumaga:
I totonu o se fagu mamafa mafolafola ma le ufiufi gutu o le eleele ma le mamafa faifaipea ma le faʻamago, fua le 1 kalama o faʻataʻitaʻiga (saʻo i le 0.0001 kalama).O le faʻataʻitaʻiga e tatau ona faʻasalalau solo i le pito i lalo o le fagu mamafa ma le mafiafia e le sili atu i le 10mm, tuʻu i totonu o se ogaumu faʻamago eletise eletise, faʻamago i le 105-110 ℃ mo le 3 itula, ona alu lea i totonu o le potu faʻagogo, malulu. i le vevela potu ma fua.
Fuafuaga: Pa'u mamafa mago %= (M1-M2) ÷M×100
O fea: M1: mamafa o fa'ata'ita'iga ma fagu mamafa a'o le'i fa'amago, g
M2: Massa o faʻataʻitaʻiga ma fagu mamafa pe a uma ona faʻamago, g
M: Fa'ata'ita'i mamafa, kalama
5. Taamilomilo faapitoa
-- E fuaina le suiga fa'apitoa e tusa ai ma le GB/T613-1988
Sauniuniga faʻataʻitaʻiga: 0.5000g o faʻataʻitaʻiga na fuaina saʻo ma tuʻuina atu i se fagu volumetric mama ma mago 50ml ma le 20ml Ethyl acetate.Sa pupuni le fagu ma lulu e fa'amuta ai le Ethyl acetate.
Su'ega: Fetuuna'i le zero o le gyroscope a'o le'i faia le su'ega, ona fa'atumu lea o le paipa su'ega i fa'ata'ita'iga vaifofo, fa'amaumau le Deflection Angle o le taamilosaga, ma fuafua le suiga patino o le fa'ata'ita'iga e fa'aaoga ai le fua fa'atatau.
[α]20/D= (r×50) ÷(L×W)
O fea:
[α]20/D: Va'aiga fa'apitoa ole vaifofo fa'ata'ita'i ile 25℃
r: Va'aia le suiga ile 25℃ mo fa'ata'ita'iga fofo
50: Saunia fa'ata'ita'iga vaifofo voluma (ml)
w: Fa'ata'ita'iga mamafa (g)
L: umi o paipa fa'amata (dm)
6. Su'esu'ega TCL
Sauniuniga faʻataʻitaʻiga: 0.5000g faʻataʻitaʻiga saʻo le fuaina ma faʻanofo i se fagu 50ml volumetric mama ma mago, 40ml EtoH na faʻaopoopoina, faʻapipiʻi le fagu ma luluina e faʻamavae, ona faʻafefe ai lea i le fua faʻatasi ma EtoH.
Sauniuniga o le faʻataʻitaʻiga faʻataʻitaʻiga: 0.5000g faʻataʻitaʻiga na saʻo le fuaina ma faʻanofo i se fagu volumetric mama ma mago 50ml, 40ml vai ma 2 mataua o le 6N hydrochloric acid na faʻaopoopoina, faʻapipiʻi le fagu ma luluina e faʻamavae, ona faʻafefiloi lea i le fua ma vai.Ona ave lea o le 0.5 o le vai o loʻo i luga ma faʻafefe i le ethanol i totonu o se fagu voluma 100ml i se faʻataʻitaʻiga faʻataʻitaʻiga ma 1.0% amino acid toega fua.
10 microliter faʻataʻitaʻiga na ave i se microsampling syringe, ma faʻataʻitaʻiga na tuʻuina i luga o le GF254 silicone plate.5 microliter, 10 microliter ma 15 microliter point plates na ave mo faʻatusatusaga, faasologa.
Su'i fa'atupuina: n-butanol: fa'a'aisa acetic glacial: vai =4:1:1.
Su'i fa'atupu lanu: 5% ninihydrin methanol solution.
Fa'agaioiga fa'atupu lanu: muamua, fa'aoga se mea fa'agogo lauulu e fa'agogo ai le ipu silica gel ua atia'e, ona fufui lea i le 5% ninhydrin methanol solution, ona feula lea, ma tao i le vevela maualuga i totonu o le ogaumu eletise mo le atina'eina o lanu.
Fa'amasinoga: Va'ai i le taimi lanu o le oloa, le tele o le va'aiga, le fa'avasegaina o le lanu ma le fa'atusatusaga ma le oloa fa'atonutonu, fa'amasino o lo'o i totonu o lena vaega.