L-Cysteine ​​Hydrochloride Monohydrate CAS 7048-04-6 Su'ega 98.5~101.0% (Titration on Dried Basis) Falegaosimea

L-Cysteine ​​Hydrochloride Monohydrate (CAS: 7048-04-6) Metotia Su'ega AJI97
Fa'ailoaina: Fa'atusatusa le fusi o le fa'ata'ita'i fa'ata'ita'i fa'atasi ma le fa'ata'ita'iga e ala i le metotia tisiketi o le potassium bromide.
Ta'amilosaga Fa'apitoa [α]D20: Fa'ata'ita'i mago, C=8, 1mol/L HCl
Setete o Fofo (Transmittance): 1.0g i le 10ml o le H2O, spectrophotometer, 430nm, 10mm mafiafia sela.
Chloride (Cl): 350mg, B-2
Ammonium (NH4): A-1
Sulfate (SO4): 1.2g, (1), ref: 0.50ml o le 0.005mol/L H2SO4
Uamea (Fe): 1.5g, ref: 1.5ml o Iron Std.(0.01mg/ml)
Metala Mamafa (Pb): 2.0g, (1), pH=7, ref: 2.0ml o Pb Std.(0.01mg/ml)
Arsenic (As2O3): 2.0g, (1), ref: 2.0ml o As2O3 Std.
Isi Amino Acids: Faʻataʻitaʻiga Suʻega: 50μg, B-6-a, pulea;L-Cys·HCl·H2O 0.25μg
Ma'imau ile Fa'agogo: I totonu o le vacuum, P2O5, ile vevela ole potu mo le 20 itula.
Toega i luga o le mumu (Sulfated): Su'ega AJI 13
Su'ega: Fa'ata'ita'iga mago, 250mg, 0.05mol/L I2 1ml=15.762mg C3H7NO2S·HCl
Su'ega pH: 1.0g ile 100ml H2O

L-Cysteine ​​Hydrochloride Monohydrate (CAS: 7048-04-6) USP35 Metotia Su'ega
FAAMATALAGA
Cysteine ​​Hydrochloride o le L-Cysteine ​​Hydrochloride Monohydrate ma o loʻo i ai le NLT 98.5% ma le NMT 101.5% o le L-Cysteine ​​Hydrochloride (C3H7NO2S·HCl), fuafua i luga o le faʻamago.
FAATINOGA
A. FA'AULA'I INFRARED <197K>
SU'ESU'EGA
TULAGA
Fa'ata'ita'iga: 250 mg o le Cysteine ​​Hydrochloride
Avanoa: Faʻaauau e pei ona faʻatonuina i le Suʻesuʻega e aunoa ma le Faʻataʻitaʻiga.
Titrimetric faiga
(Siaki Titrimetry <541>)
Auala: Toe fa'asusuga
Titrant: 0.1 N iodine VS
Fa'asuavai tua: 0.1 N sodium thiosulfate VS
Su'esu'ega pito: Va'ai
Iloiloga: Fa'aliliu le Fa'ata'ita'i ile fagu iodine.Faaopopo le 20.0 mL o le vai ma le 4 g o le potassium iodide, ma faʻafefiloi.Fa'alili le vaifofo ile ta'ele aisa, ma fa'aopopo le 5 mL ole 3 N hydrochloric acid ma le 25.0 mL ole 0.1 N iodine VS.Fa'aofi le taofi, ma fa'ataga e tu i le pogisa mo le 20 min.Fa'asusu le iodine tele i le fa'asu'u i tua.Fa'aopoopo le 3 mL o fetu ch TS a'o latalata atu le pito i'uga.E tusa ai ma le filifiliga avanoa.Fa'atatau le pasene o le cysteine ​​hydrochloride (C3H7NO2S·HCl) i le Fa'ata'ita'iga na ave:
I'uga = {[(VB − VS) × N × F]/W} × 100
VB = Tu'u titrant voluma fa'aaoga e le Avanoa (mL)
VS = Tu'u titrant voluma fa'aaoga e le Fa'ata'ita'iga (mL)
N = tulaga masani o le fa'asolo i tua (mEq/mL)
F= fua tutusa, 157.6 mg/mEq
W= Fa'ata'ita'i mamafa (mg)
Tulaga taliaina: 98.5% ~ 101.5% i luga o le faʻamago
LELEI
TOE FA'AVA'E I LE FA'AVAE <281>: NMT 0.4%
CHLORIDE MA SULFATE, Sulfate <221>
Fofo masani: 0.10 mL o le 0.020 N sulfuric acid
Fa'ata'ita'iga: 0.33 g o le Cysteine ​​Hydrochloride
Ta'iala e talia: NMT 0.03%
Umea <241>: NMT 30ppm
METAL MAMAFA, Metotia I <231>: NMT 15ppm
FA'AFEFEAGAI FA'AVAE
N-Ethylmaleimide solution: 40 mg/mL o le N-ethylmaleimide ile ava malosi
Fofo fa'asoa masani: Fa'amuta 20 mg o le USP L-Cysteine ​​Hydrochloride RS i le 10.0 mL vai.Fa'aopoopo le 10.0 mL ole vaifofo N-Ethylmaleimide, ma fa'afefiloi.Fa'ataga le vaifofo e tu mo le 5 min a'o le'i fa'aogaina.
Fofo masani: 0.05 mg/mL mai le vaifofo masani o oloa.[ FAAMANATU-O lenei vaifofo o loʻo i ai le faʻatonuga e tutusa ma le 0.5% o le vaifofo Faʻataʻitaʻi.]
Fofo talafeagai o le faiga: Fa'aliliu le 10 mg o le USP L-Tyrosine RS ma le 10 mL o le vaifofo fa'asoa masani i se fagu volumetric 25-mL.Fa'asusu i le vai i le voluma.
Fa'ata'ita'iga vaifofo: Fa'aliliu le 0.2 g o le Cysteine ​​Hydrochloride i se fagu volumetric 10-mL, fa'amavae, ma fa'afefe i le vai i le voluma.
Fa'ata'ita'iga fofo: I le 5.0 mL o le fa'ata'ita'iga fa'asoa fa'asoa fa'aopoopo le 5.0 mL ole N-Ethylmaleimide solution, ma fa'afefiloi.Fa'ataga le vaifofo e tu mo le 5 min a'o le'i fa'aogaina.
Fa'asologa o ata (Va'ai Chromatography <621>, Chromatography Layer Manifinifi.)
Auala: TLC
Adsorbent: 0.25-mm apa o chromatographic silica gel paluga
Ole tele ole talosaga: 5μL
Fa'atupuina o le soliga: Butyl alcohol, glacial acetic acid, ma le vai (3:1:1)
Fa'asu'u mea fa'afefe: 2 mg/mL o le ninhydrin i le fa'afefiloi ole butyl alcohol ma le 2N acetic acid (95:5)
Faiga talafeagai
Mana'oga talafeagai: O le chromatogram o le Fa'atonuga fa'aoga fofo o lo'o fa'aalia ai ni vaega manino se lua.
Iloiloga
Fa'ata'ita'iga: Fa'ata'ita'iga fa'ata'ita'iga, Fa'atonuga fa'aoga fofo, ma Fa'ata'ita'iga fofo.
A maeʻa ona faʻamago le ipu, faʻafefe i le Spray reagent, ma vevela i le va o le 100 ° ma le 105 ° mo le 15 min.Su'e le ipu i lalo o le malamalama pa'epa'e.
Ta'iala e talia ai: So'o se vaega lona lua o le Fa'ata'ita'iga fofo e le sili atu pe sili atu le malosi nai lo le nofoaga autu o le fofo Fa'ata'ita'i.
Fa'aletagata ta'ito'atasi: NMT 0.5%
Aofa'iga o mea leaga: NMT 2.0%
SU'E FA'AMANATU
FILI'IGA FA'AVAE, Ta'amilosaga Fa'apitoa <781S>
Fa'ata'ita'iga fofo: 80 mg/mL ile 6 N hydrochloric acid
Tulaga taliaina: +5.7° i le +6.8°
FAAMAGO LOTO <731>: Fa'amago se fa'ata'ita'iga i le vevela o le potu i le mamafa e le sili atu i le 5 mm o le mer cury mo le 24 itula: e leiloa le 8.0% ~ 12.0% o lona mamafa.
FAAOPOOPOGA
FA'UFOGA MA TE'UINA: Fa'asao i totonu o pusa tapuni lelei.
USP REFERENCE STANDARD <11>
USP L-Cysteine ​​Hydrochloride RS
USP L-Tyrosine RS