L-Leucine Methyl Ester Hydrochloride CAS 7517-19-3 (H-Leu-OMe·HCl) Su'ega >99.0% Falegaosimea

1. Folafolaga
Ave se aofaiga talafeagai o lenei oloa, tuu i luga o se pepa paepae lamolemole ma faasalalau 5 cm2 ma 5 mm mafiafia.Matauina i se nofoaga susulu.
2. Mama (HPLC)
2.1 Meafaigaluega
Ole chromatography vai maualuga, su'esu'e PDA.
Paleni au'ili'ili faaeletonika
2.2 Reagents
Acetonitrile (vaega chromatographic), TFA (vaega chromatographic)
2.3 Tulaga Chromatographic
2.3.1 Koluma: YMC-ODS-AM, 5 μl, 150 x 4.6 mm
2.3.2
Sailiga umi galu: INC220nm
Fuafuaga tafe: 1.0mL/min
Volume tui: 10 μL (mo faʻamatalaga)
Suavai: Acetonitrile
Taimi ta'avale: 20 minute
2.4 Sauniuniga vaega feaveai
Vaega feavea'i A (0.1% trifluoroacetic acid vai): sa'o mitiia 2.0ml TFA, Fa'amama i le 2000ml i le vai, fa'afefiloi ma degas;
Laasaga feavea'i B (0.1% trifluoroacetic acid acetonitrile ): fa'asa'o sa'o le 2.0ml TFA, Fa'amama i le 2000ml ma le acetonitrile, fa'afefiloi ma degas;
2.5 Fa'asologa o meafaigaluega
Taimi (min) A% B%
0.00 90 10
12.00 10 90
15.00 10 90
15.01 90 10
20.00 90 10
2.6 Sauniuniga o fofo faataitai
Fua ma fa'amuta 0.1g fa'ata'ita'iga fa'atasi ai ma le acetonitrile, fa'afefete i le 100ml, lulu lelei mo le fa'aoga, po'o le fa'atonuga tutusa.Saunia ni faataitaiga se lua e tutusa.
2.7 Fa'ata'ita'iga Filifiliga
Iloilo le fa'ata'ita'iga e tusa ai ma faiga fa'ata'ita'iga nei:
E silia ma le 1 tui ole vaifofo avanoa
1 nila fa'ata'ita'iga fofo 1#
1 nila fa'ata'ita'iga fofo 2#
2.8 I'uga Fa'atatau
2.8.1 Na fa'aogaina le fa'atama'ia o le vaega pito i luga e fa'atatau i le mama o le HPLC e ala i le toese o avanoa avanoa.
2.8.2 Ole eseesega ole fa'atatau ole mama ole nila e lua e le tatau ona sili atu ile 1%
2.8.3 Afai o taunuʻuga o tui e lua e fetaui ma tulaga taliaina, o le averesi o le mama e avea ma taunuuga mulimuli.
3, Mea liusuavai -- RY-1 mea fa'ameamea
4. gau ile fa'amago metotia su'ega
4.1 Meafaigaluega:
Omea fa'amago fa'amago eletise, 1/10,000 paleni.
4.2 Taualumaga:
I totonu o se fagu mafolafola mamafa ma le mamafa faifaipea ma le fa'amamago le ufiufi gutu o le eleele, fua le 1 kalama (sa'o i le 0.0001 kalama) o le fa'ata'ita'iga.O le faʻataʻitaʻiga e tatau ona faʻasalalau solo i le pito i lalo o le fagu mamafa ma le mafiafia e le sili atu i le 10mm, tuʻu i totonu o se ogaumu faʻamago eletise eletise, faʻamago i le 105 ~ 110 ℃ mo le 3 itula, ona alu lea i totonu o le potu faʻagogo e malulu i vevela potu mo le fuaina.
Fa'atatau: Ma'imau ile fa'amago %= (M1-M2) ÷M×100
O fea: M1: mamafa ole fa'ata'ita'iga ma le fagu fua a'o le'i fa'amago, kalama
M2: mamafa ole fa'ata'ita'iga ma le fagu fua pe a uma ona fa'amago, ile kalama
M: Fa'ata'ita'i mamafa, kalama
5. Taamilomilo faapitoa
-- E fuaina le suiga fa'apitoa e tusa ai ma le GB/T613-1988
Sauniuniga faʻataʻitaʻiga: 1.0000g faʻataʻitaʻiga saʻo le fuaina ma faʻanofo i se fagu volumetric mama ma mago 50ml, 20ml vai na faʻaopoopoina, faʻapipiʻi le fagu ma luluina e faʻamavae, ona faʻafefe ai lea i le fua i le vai.
Su'ega: Fetuuna'i le zero o le gyroscope a'o le'i faia le su'ega, ona uta lea o le paipa su'ega ma le fa'ata'ita'iga vaifofo, fa'amaumau le Angle rotation, ma fuafua le suiga patino o le fa'ata'ita'iga ma le fua fa'atatau.
[a]D20 = (r×50) ÷ (L×W)
[a]D20: Va'aiga fa'apitoa fa'apitoa ile 20℃ ole fa'ata'ita'iga fofo
r: O le suiga o le mata ua matauina i le 20 ℃ mo le faʻataʻitaʻiga fofo
50: Volume o fofo fa'ata'ita'i saunia (ml)
w: Fa'ata'ita'iga mamafa (g)
L: Umi o paipa fe'avea'i mata'utia (dm)