Phthalimide Potassium Salt CAS 1074-82-4 Mama >99.0% (HPLC)

3. Su'ega metotia
3.1 Ma'imau ile Fa'amago (W/W)
3.1.1 Meafaigaluega ma meafaigaluega
Tasi i le sefulu afe o paleni faaeletonika, ogaumu faʻamago eletise eletise, 40 * 25 fagu mamafa, faʻamago
Reagent: silica gel
3.1.2 Metotia
1. Fa'amama 3 40*25 fagu mamafa.
2, tuu ile ogaumu ile 105℃ (±2℃) mago mo le 1 itula.
3. Aveese le fagu 40 * 25 mamafa ma tuʻu i totonu o le faʻamago e faʻamalo mo le 30min.
4, Sa'o le mamafa 40*25 fua fagu gaogao mamafa W0.
5. Fa'aaoga se fagu mamafa 40*25 e fua ai le fa'ata'ita'iga fa'afefiloi tutusa o le 3.0000g (W1), o le aofa'i o le 3.
6, Tuu i totonu o le ogaumu, aveese le pulou fagu, tuʻu i tafatafa o le fagu mamafa, poʻo le afa tatala le fagu fagu mo le faʻamago, ave i fafo, e tatau ona lelei le mamafa o fagu, faʻamago i le 105℃ (± 2℃) mo 3 itula.
7. Aveese le fagu mamafa 40 * 25 ma tuʻu i totonu o le masini faʻamago e faʻamalo mo le 30min.
8, Saʻo le mamafa pe a uma ona faʻamago 40 * 25 mamafa o le fagu W2.
Fuafuaga fa'atatau: Ma'imau ile Fa'amago (%) =(W1-W2)/(W1-W0)×100%
3. 4 Filifiliga o mataupu
3.4.1 Mea faigaluega ma meafaigaluega
LC-UV100 Liquid Chromatograph (Shanghai Wufeng Scientific Instrument Co., LTD.)
10ml fagu volumetric, sipuni tulimanu uamea stainless ma isi nofoaga e fesootai i ai
3.3.2 Metotia
Metotia mo le suʻesuʻeina o le Phthalimide Potassium Salt
3.3.3 Suavai chromatography
Tulaga Chromatographic
Koluma Chromatographic: HypersilC6H5 (C8 avanoa foi) 4.6*250mm 5um
Sailiga umi galu: 210nm
Malosi o le koluma: 30℃
Fuafuaga tafe: 1.0ml / min
Fa'ata'ita'iga fa'atonuga: 0.05mg/ml (diluent ole vaega feavea'i)
Reagents
Potassium Phosphate Dibasic(su'esu'e mama)
Phthalimide Potassium Salt (fa'ata'ita'iga masani)
Acetonitrile (su'esu'e mama)
Vai mama
Vaega fe'avea'i: acetonitrile: phosphate buffer (PH:3.5) =40:60
Fua 1.63g Potassium Phosphate Dibasic ma solo i le 600ml vai mama, fetuutuunai le tau o le PH i le 3.5 ma le phosphoric acid, sasaa i le 1000ml beaker o loʻo i ai le 400ml acetonitrile, faʻafefiloi lelei, faamama ma 0.45um fefiloi fibre fibre membrane, solo ma standby vibration. talosaga.
3.3.4 Taualumaga
1) Fofo su'ega fa'atulagaina (0.05mg/ml)
O le faʻataʻitaʻiga 2.5mg na saʻo le fuaina ma tuʻu i totonu o se fagu volumetric 50ml.O le vaega feaveaʻi na faʻaopoopoina ma faʻafefe i le fua e ala i le faʻamavaeina o le ultrasonic.
2) Fa'atonu fofo masani (1.0mg/ml)
O le 2.5mg faʻataʻitaʻiga faʻataʻitaʻiga na saʻo le mamafa ma tuʻu i totonu o se fagu volumetric 50ml.O le vaega feaveaʻi na faʻaopoopoina ma faʻafefe i le fua e ala i le faʻamavaeina o le ultrasonic.
3) Fa'aoga fa'aoga:
(1) Tuu le ulu faamama i le 80% methanol i le amataga, tatala le alavai faʻafefe ma faʻafefe mo le 3-5min, ma tapuni le alavai faʻafefe ma fufulu mo le 10-15min.Ona tuʻu lea o le ulu faamama ile 90% vai mama, tatala le alavai alavai ma faʻafefe mo le 3-5min, tapuni le alavai ma fufulu mo le 10min.
(2) Ona sui lea o le vaega feaveaʻi saunia, o le tafe o le 1.0ml / min, o le koluma pito i luma o le mamafa e itiiti ifo i le 1000Psi, tatala le mea e iloa ai, faʻatali seia oʻo ina mautu le koluma pito i luma, tatala le fale faigaluega komepiuta, ma ulufale i luga ole laiga.
(3) A maeʻa ona mautu le mamafa o le koluma, tatala le mea e iloa ai.Tu'i muamua le nila 1 o le vaega feavea'i, ona tui lea o le nila 1 o le fa'ata'ita'iga fa'ata'ita'i, ma fa'ai'u ai le tui fa'ata'ita'iga.
(4) Fa'ai'uga fa'atatau: auala e fa'avasega ai le eria
(5) A maeʻa le galuega suʻesuʻe, tapuni muamua le mea e iloa ai, ona fufulu lea o le ulu faamama ile vai mama mo le 10-15min;I le taimi lava e tasi, o le tui tui na fufulu i le vai ma le methanol.
(6) Mulimuli ane, tu'u le 80% methanol, fufulu mo le 30 minute, ma tapuni.
4. A maeʻa le asiasiga, faʻatumu le suʻega ma le suʻega suʻega suʻega.