Acesulfame K CAS 55589-62-3 Acesulfame Potassium Purity > 99.0% (HPLC)

DEFINITION
Acesulfame Potassium ine NLT 99.0% uye NMT 101.0% yeC4H4NO4SK, yakaverengerwa payakaomeswa.
KUZIVA
• A. INFRARED ABSORPTION <197K>
• B. ZVIYEDZO ZVOKUZIVISA-GENERAL, Potassium <191>
Sample solution: 100 mg/mL
Maitiro ekugamuchira: Inosangana nezvinodiwa
ASSAY
• MAITIRO
Muenzaniso: 150 mg
Titrimetric system (Ona Titrimetry <541>)
Mode: Direct titration
Titrant: 0.1 N perchloric acid VS
Chisina chinhu: 50 mL yeglacial acetic acid
Endpoint kuona: Potentiometric
Ongororo: Dissolve Sample mu50 mL yeglacial acetic acid.
Titrate ine 0.1 N perchloric acid VS.Ita chisungo chisina chinhu.
Verenga iyo muzana yeacesulfame potassium (C4H4NO4SK) muSample:
Mhedzisiro = [(V − B) × N × F × 100]/W
V = titrant vhoriyamu inopedzwa neSample (mL)
B = titrant vhoriyamu inopedzwa neiyo Blank (mL)
N = titrant chaiyo normality (mEq/mL)
F = yakaenzana chinhu, 201.2 mg/mEq
W = uremu hweSample (mg)
Maitiro ekugamuchira: 99.0% -101.0% pane yakaomeswa nheyo
KUSIRIRA
• LIMIT OF FLUORIDE
[Cherechedza-Shandisa plasticware panguva yese iyi bvunzo.]
Mhinduro A: Dissolve 210 g ye citric acid monohydrate mu 400 mL yemvura.Rongedza neconcentrated ammonia kusvika pH ye7.0, uye dilue nemvura kusvika ku1000 mL.
Mhinduro B: 132 mg/mL ye dibasic ammonium phosphate
Solution C: Kuturika kwe292 g edetic acid mu500 mL yemvura, wedzera 200 mL yeammonium hydroxide, gadzirisa neammonium hydroxide kusvika pH iri pakati pe6 ne7, uye dilute nemvura kuita 1000 mL.
Buffer solution: Sanganisa mavhoriyamu akaenzana eSolution A, Solution B uye Solution C, wogadzirisa neammonium hydroxide kusvika pH ye7.5.
Standard stock solution: Wema 0.442 g ye sodium fluoride, yakamboomeswa pa 300 ° kwemaawa gumi nemaviri, mu 1-L volumetric flask, uye dilue nemvura kusvika kuhuwandu.Chengetedza mhinduro mumudziyo wepurasitiki wakavharwa.Pakarepo usati washandisa, pipet 5 mL yemhinduro iyi mu 100-mL volumetric flask, uye dilue nemvura kusvika kuhuwandu.Imwe mL yemhinduro iyi ine 10µg ye fluoride ion.
Standard solution A: Sanganisa 0.5 mL yeStandard stock solution uye 15.0 mL yemushonga weBuffer, wozodzikisa nemvura kusvika pa50mL.
Mushonga wakajairika B: Sanganisa 1.0 mL yeStandard stock solution uye 15.0 mL yeBuffer solution, wozodzikisa nemvura kusvika pa50mL.
Mushonga wakajairwa C: Sanganisa 1.5 mL yeStandard stock solution uye 15.0 mL yeBuffer solution, wozodzikisa nemvura kusvika pa50mL.
Standard solution D: Sanganisa 3.0 mL yeStandard stock solution uye 15.0 mL yemushonga weBuffer, wobva wawedzera nemvura kusvika pa50mL.
Sample solution: Ku50-mL volumetric flask wedzera 3 g yeAcesulfame Potassium.Dissolve mumvura, wedzera 15.0 mL yeBuffer solution, uye wedzera nemvura kusvika kuhuwandu.
Analysis
Samples: Standard solution A, Standard solution B, Standard solution C, Standard solution D, uye Sample solution
Sanganisa kuyera zvinogona (ona Titrimetry <541>) mumV, yeStandard solutions uye Sample solution, ine pH metre yakakodzera ine fluoride-specifiion electrode uye silver-silver chloride reference electrode.Paunenge uchitora zviyero, endesa mhinduro kune 25-mL beaker, uye kunyudza magetsi.Isai bhaa rine polytef-yakavharidzirwa inobvongodza mubeaker, isa beaker pane magnetic stirrer ine insulated top uye wobvumira kubvongodza kusvika kuenzana kwawanikwa (1-2 min).Geza, uye woomesa maelectrodes pakati pezviyero, uchichenjerera kuti usakwenya kristaro mufluoride-specific ion electrode.Edza kugona kwemhinduro yega yega yega yega, uye rongedzera huwandu hwefluoride, muµg/mL, maringe nezvinogoneka, mumV, pabepa remilogarithmic.Pima kugona kwemushonga weSample, uye tarisa huwandu hwefluoride kubva pachiyero chakajairwa, muµg/mL.
Verenga zviri mukati, muppm, zvefluoride muchikamu cheAcesulfame Potassium chakatorwa:
Mhedzisiro = (V × C/W)
V = vhoriyamu yeSample solution (mL)
C = kusangana kwefluoride muSample solution, kubva kune yakajairwa curve (mg/mL)
W = huremu hweAcesulfame Potassium yakatorwa kugadzirira Sample solution (g)
Maitiro ekugamuchira: NMT 3 ppm
• METARI ZVIKURU, Nzira I <231>: NMT 10 ppm
• KUCHENA KWEKROMATOGRAPHIC
Mhinduro A: 3.3 mg/mL ye tetrabutylammonium hydrogen sulfate
Nharembozha chikamu: Acetonitrile uye Solution A (2:3)
Sistimu yakakodzera mhinduro: 2 µg/mL imwe neimwe yeUSP Acesulfame Potassium RS uye ethylparaben
Yakajairika mhinduro: 0.2 µg/mL yeUSP Acesulfame Potassium RS
Sample solution: 10 mg/mL
Chromatographic system
(Ona Chromatography <621>, System Suitability.)
Modhi: LC
Detector: UV 227 nm
Column: 4.6-mm × 25-cm;5-µm kurongedza L1
Kuyerera: 1 mL / min
Jekiseni saizi: 20 µL
Kukodzera kwehurongwa
Sample: System kukodzera mhinduro
Kukodzera zvinodiwa
Kugadzirisa: NLT 2 pakati peacesulfame potassium uye ethylparaben
Analysis
Samples: Yakajairwa mhinduro uye Sample mhinduro
Nyora ma chromatograms kwenguva yekumhanya NLT 3 nguva yekuchengetedza yeacesulfame potassium peak, uye kuyera mhinduro dzenharaunda dzepamusoro.
Maitiro ekugamuchira: Mhinduro yechero peak pane imwe nguva yekuchengetedza kunze kweiyo acesulfame potassium kubva kuSample solution haipfuure mhinduro yeacesulfame potassium peak kubva kuStandard solution (0.002%).
ZVIYEDZO ZVAKANAKA
• ACIDITY KANA KUNYANYA
Sample solution: 4.0 g mu 20 mL ye carbon dioxide-isina mvura
Kuongorora: Wedzera 0.1 mL yebromothymol yebhuruu TS.Kana mhinduro iri yero, titrate ne 0.01 N sodium hydroxide kuburitsa ruvara rweblue.Kana mhinduro iri yebhuruu, titrate ne 0.01 N hydrochloride acid kuti ibudise ruvara rweyero.
Maitiro ekugamuchira: NMT 0.2 mL ye0.01 N sodium hydroxide kana NMT 0.2 mL ye0.01 N hydrochloric acid inodiwa.
• KURASIKA PAKUOMESA <731>: Pukuta sampuli pa105° kwe3 h: inorasikirwa neNMT 1.0% yehuremu hwayo.
ZVIMWE ZVINODIWA
• KUPAKA UYE KUCHENGESHA: Chengetedza mumudziyo wakavharwa zvakanaka, uye dzivirira pachiedza.Chengetedza pane tembiricha yekamuri.
USP REFERENCE MITIRO <11>
USP Acesulfame Potassium RS