Adenine CAS 73-24-5 Assay 98.0% ~ 102.0% (Titration) High Purity Factory

Adenine ine NLT 98.0% uye NMT 102.0% yeC 5H5N5, yakaverengerwa payakaomeswa.
KUZIVA
A. INFRARED AVORPTION <197K>
ASSAY
PROCEDURE
Muenzaniso: 200 mg yeAdenine
Chisina chinhu: 80 mL yemusanganiswa we100 mL yeglacial acetic acid uye 300 mL yeacetic anhydride
Titrimetric System
Mode: Direct titration
Titrant: 0.1 N per chloric acid VS yakagadziriswa sezvinotevera: Kuendesa 300 mg ye potassium biphthalate kune 150-mL beaker uye, nekukurudzira, kunyunguduka mu80 mL yemusanganiswa we100 mL yeglacial acetic acid uye 300 mL yeacetic anhydride.Titrate neper chloric acid solution.Imwe neimwe 20.42mg ye potassium biphthalate yakaenzana ne 1 mL ye 0.1 N perchloric acid.
Endpoint kuona: Potentiometric
Ongororo: Dissolve Sample mu80 mL yemusanganiswa we100 mL yeglacial acetic acid uye 300 mL yeacetic anhydride nekukurunga, uye titrate neTitrant.Verenga chikamu chezana cheAdenine (C5H5N5) muchikamu chakatorwa:
Mhedzisiro = [(V − B) × N × F × 100]/W
V = Sample titrant volume (mL)
B = Vhoriyamu isina chinhu (mL)
N = titrant normality (mEq/mL)
F = yakaenzana chinhu, 135.13 mg/mEq
W = uremu hweSample (mg)
Maitiro ekugamuchira: 98.0% -102.0% pane yakaomeswa
KUSIRIRA
Inorganic Impurities
RESIDUEON IGNITION <281>: NMT 0.1%
ZVINORERA METALS, Nzira II <231>: NMT 10ppm
Organic Kusachena
PROCEDURE
pH 7.0 phosphate buffer: Dissolve 4.54 g ye monobasic potassium phosphate mumvura kuita 500 mL yemhinduro.Dissolve 4.73 g ye anhydrous dibasic sodium phosphate mumvura kuita 500 mL yemhinduro.Sanganisa 38.9 mL ye monobasic potassium phosphate solution ne 61.1 mL ye dibasic sodium phosphate solution.Gadzirisa, kana zvichidikanwa, nekuwedzera kwedonhwe re dibasic sodium phosphate mhinduro kune pH ye7.0.
Standard stock solution: Dissolve akakodzera uwandu hweUSP Adenine RS mumvura inopisa, inotonhorera, uye dilute quantitatively nemvura kuti uwane mhinduro ine chiyero chinozivikanwa che 0.19 mg/mL.
Standard solutions: Pipet 5-mL zvikamu zveStandard stock solution mumatatu 100-mL volumetric flasks, uye dilute ne 0.10 N hydrochloric acid, 0.010 N sodium hydroxide, uye pH 7.0 phosphate buffer, zvichiteerana, kusvika kuhuwandu.
Sample stock solution: Dissolve huwandu hwakakodzera hweAdenine mumvura inopisa, inotonhorera, uye diluted quantitatively nemvura kuti uwane mhinduro ine huwandu hunozivikanwa hwe0.19 mg/mL.
Sample stock solution: Dissolve huwandu hwakakodzera hweAdenine mumvura inopisa, inotonhorera, uye diluted quantitatively nemvura kuti uwane mhinduro ine huwandu hunozivikanwa hwe0.19 mg/mL.
Sample solutions: Pipet 5-mL zvikamu zveSample stock solution mumatatu 100-mL volumetric flasks, uye dilute ne0.10 N hydrochloric acid, 0.010 N sodium hydroxide, uye pH 7.0 phosphate buffer, zvichiteerana, kusvika kuhuwandu.
Spectrometric mamiriro
(Ona Spectrophotometry uye Chiedza-Kuparadzira <851>.)
Modhi: UV-Vis
Wavelegth kureba: 220-320 nm
Sero: 1 cm
Chisina chinhu: Mvura
Analysis
Samples: Yakajairwa mhinduro uye Sample mhinduro
Maitiro ekugamuchira: Iwo anoenderana absorptivities, akaverengerwa payakaomeswa hwaro, pawavelengths yekunyanyisa kunyura, kune imwe neimwe yemhinduro dzinoenderana haina kusiyana neinopfuura 2.0%.
ZVIYEDZO ZVAKANAKA
LOSSON DRYING <731>: Dry sampuli pa 110 ° kwe4 h: inorasikirwa neNMT 1.0% yehuremu hwayo.
NITROGEN CONTENT, Nzira II 〈461〉: 50.2% -53.4%, yakaverengerwa pane yakaomeswa
ZVIMWE ZVINODIWA
PACKAGE AND STORAGE: Chengetedza mumidziyo yakavharwa zvakanaka.
USP REFERENCE MITIRO <11>
USP Adenine RS