Adenosine CAS 58-61-7 Assay 99.0% -101.0% USP Standard Factory High Purity

DEFINITION
Adenosine ine NLT 99.0% uye NMT 101.0% yeC10H13N5O4, yakaverengerwa payakaomeswa.
KUZIVA
• A. INFRARED ABSORPTION <197K>: NMT 0.1%
ASSAY
• ADENOSINE
Muenzaniso: 200 mg yeAdenosine yakamboomeswa pa105 ° ye2h
Titrimetric system
(Ona Titrimetry <541>)
Mode: Direct titration
Titrant: 0.1 N per chloric acid VS
Endpoint kuona: Potentiometric
Ongororo: Dissolve mu50 mL yeglacial acetic acid uye titrate ine 0.1 N per chloric acid VS.Verenga chikamu chezana cheAdenosine (C10H13N5O4) muchikamu chakatorwa:
Mhedzisiro = [(V − B) × N × F × 100]/W
V = Sample titrant volume (mL)
B = Vhoriyamu isina chinhu (mL)
N= titrant normality (mEq/mL)
F= equivalency factor: 267.25 mg/mEq
W = uremu hweSample (mg)
Maitiro ekugamuchira: 99.0% -101.0% pane yakaomeswa hwaro
• KUSINA
• KUBATSIRA KWASARA <281>: NMT 0.1%
• METARI ZVIKURU, Nzira II <231>: NMT 10 ppm
• LIMIT OF AMMONIA
Sample solution: Simbisa 0.5 g mu 10 mL yemvura.Kurudzira 30s, uye pfuura nepakati pesefa yakaoma.Dirai filtrate nemvura kusvika 15 mL, uye shandisa filtrate.
Yakajairika mhinduro: 0.4 µg/mL yeammonium chloride mumvura
Ongororo: Kumhinduro yeSample uye mhinduro yeStandard wedzera 0.3 mL yealkaline mer curic-potassium iodide TS, vhara machubhu ebvunzo, uye bvumira kumira kwemaminetsi mashanu.
Maitiro ekugamuchira: Mushonga weSample hauratidzi ruvara rweyero yakanyanya kupfuura iyo yeStandard solution (NMT 4 ppm yeammonia).
• MUGARO WEKHLORIDE
Sample solution: Simbisa 0.2 g mu 10 mL yemvura.Kurudzira 30 s, pfuura nepasefa yakakora, uye shandisa iyo filtrate.
Mushonga wakajairika: 2.3 µg/mL ye sodium chloride mumvura
Ongororo: Kumushonga weSample uye 10 mL yeStandard solution wedzera 1 mL yenitric acid uye 1 mL yesirivha nitrate TS, uye dilute mhinduro yega yega nemvura kusvika ku40 mL.Rega zvigadziriswe zvimire kwemaminitsi mashanu, zvakachengetedzwa kubva pachiedza.
Maitiro ekugamuchira: Kana ichitariswa kune yakasviba kumashure, mhinduro yeSample haina turbid kupfuura Standard solution (NMT 0.007% chloride).
• LIMIT YESULFATE
Sample solution: Regedza 0.75 g mu 15 mL yemvura.Kurudzira 30 s, pfuura nepasefa yakakora, uye shandisa iyo filtrate.
Mushonga wakajairika: Wedzera 0.15 mL ye0.020 N sulfuric acid kusvika 15 mL yemvura.
Ongororo: Kumushonga weSample uye mugadziriso weStandard wedzera 2 mL yebarium chloride TS uye 1 mL ye3 N hydrochloric acid, dilue mhinduro yega yega nemvura kusvika makumi matatu mL, uye sanganisa.Rega zvigadziriswe zvimire kwemaminitsi mashanu.
Maitiro ekugamuchira: Mushonga weSample hauna nyonganyonga kupfuura Mushonga Wenguva (NMT 0.02% sulfate).
• KUSINA ZVINHU
Mhinduro A: 6.8 g/L ye potassium hydrogen sulfate uye 3.4g/L ye tetrabutylammonium hydrogen sulfate mumvura.Gadzirisa ne2 N potassium hydroxide kusvika pH ye6.5.
Mhinduro B: 0.1 g / L ye sodium azide solution
Nharembozha chikamu: Solution A uye Solution B (60:40)
Sistimu yakakodzera mhinduro: 0.2 mg/mL imwe neimwe yeAdenosine uye inosine muMobile phase
Sample solution: 1.0 mg/mL yeAdenosine muMobile phase
Chromatographic system
(Ona Chromatography <621>, System Suitability.)
Modhi: LC
Detector: UV 254 nm
Column: 4.6-mm × 25-cm;5-µm kurongedza L1
Kuyerera kweyero: 1.5 mL/min
Jekiseni saizi: 20 µL
Kukodzera kwehurongwa
Samples: System kukodzera mhinduro
Kukodzera zvinodiwa
Kugadziriswa: NLT 9.0 pakati peadenosine uye inosine
Muswe chinhu: NMT 2.5
Kutsauswa muyero: NMT 2.0%
[Cherechedza-Chromatograph mhinduro yeSample, uye gadzirisa nguva yekumhanya kuti isvike kaviri nguva yekuchengeta yenhongonya huru.]
Analysis
Muenzaniso: Sample solution
Verenga chikamu chekusachena kwega kwega muchikamu cheAdenosine chakatorwa:
Mhedzisiro = (rU/rT) × 100
rU = peak mhinduro yekusachena yega yega kubva kuSample solution
rT = hwerengedzo yemhinduro dzese dzenhongonya dzese kubva kuSample solution
Maitiro ekugamuchira
Kusachena kwemunhu: NMT 0.1% imwe neimwe yeguanosine, inosine, uye uridine, uye NMT 0.2% yeadenine
Yese tsvina: NMT 0.5%
ZVIYEDZO ZVAKANAKA
• KUNYUNGURA RANGE KANA KUTEMIRIRA <741>: 233°-238°
• Kutenderera kweOptical, Specific Rotation <781S>: -68° kusvika -72°
Muedzo mhinduro: 20 mg/mL mu sodium hydroxide solution (1 mu20), yakatemwa pamuenzaniso wakamboomeswa pa105 ° kwe2h.
ACIDITY KANA ALKALINITY: Misa 1 g mu20 mL yemvura isina carbon dioxide.Kurudzira 30s, uye pfuura nepakati pesefa yakaoma.Kune chimwe nechimwe chezvikamu zviviri zvegumi-mL zvefiltrate wedzera 0.1mL yebromocresol yepepuru TS.
Maitiro ekugamuchira: NMT 0.3 mL ye0.01 N sodium hydroxide inodiwa kuti ibudise ruvara rweblue-violet muchikamu chimwe chete.NMT 0.1 mL ye 0.01 N hydrochloric acid inodiwa kugadzira yero mune chimwe chikamu.
•LOSSON DRYING <731>: Pukuta sampuli pa105℃ kwe2 h: inorasikirwa neNMT 0.5% yehuremu hwayo.
ZVIMWE ZVINODIWA
•KUPAKA UYE KUCHENGA: Chengetedza mumidziyo yakasimba, isingapindi chiedza, uye chengeta patembiricha yekamuri inodzorwa.
• USP REFERENCE ZVINHU <11>
USP Adenosine RS