Chenodeoxycholic Acid (CDCA) CAS 474-25-9 Assay ≥98% (Dry Basic)

Chenodeoxycholic Acid
C24H40O4: 392.57
3a,7a-Dihydroxy-5b-cholan-24-oic acid [474-25-9]
Chenodeoxycholic Acid, kana yakaomeswa, ine kwete pasi pe98.0z uye kwete kupfuura 101.0% yeC24H40O4.
Tsanangudzo Chenodeoxycholic Acid inoitika sechena, makristasi, crystalline poda kana hupfu.
Inonyungudika zvakasununguka mumethanol uye mu ethanol (99.5), inonyungudika muacetone, uye isinganyungudika mumvura.
Kuzivikanwa Sarudza iyo infrared absorption spectrum yeChenodeoxycholic Acid, yakamboomeswa, sezvakarairwa mu potassium bromide disk nzira pasi peInfrared Spectrophotometry <2.25>, uye enzanisa spectrum neReferensi Spectrum: zvese zvinotaridzika zvinoratidzira kusimba kwakafanana kwekunyura pamafungu akafanana.
Optical rotation <2.49> [a]20D : +11.0 °~ +13.0° (mushure mekuoma, 0.4 g, ethanol (99.5), 20 mL, 100 mm).
Melting point <2.60> 164~169℃
Kuchena (1) Chloride <1.03>-Dissolve 0.36 g yeChenodeoxycholic Acid mu30 mL yemethanol, wedzera 10 mL ye dilute nitric acid nemvura kugadzira 50 mL, uye ita bvunzo nemhinduro iyi.Gadzirira mhinduro yekudzora sezvinotevera: Ku 1.0 mL ye 0.01 mol/L hydrochloric acid VS wedzera 30 mL ye methanol, 10 mL ye dilute nitric acid nemvura kuita 50 mL (kwete kupfuura 0.1%).
(2) Heavy metals <1.07>-Ramba ne 1.0 g yeChenodeoxycholic Acid maererano neMethod 4, uye ita muedzo.Gadzirira mhinduro yekutonga ne 2.0 mL yeStandard Lead Solution (kwete kupfuura 20 ppm).
(3) Barium-Kusvika 2.0 g yeChenodeoxycholic Acid isa 100 mL yemvura, wofashaira kwemaminetsi maviri.Kumushonga uyu wedzera 2 mL yehydrochloric acid, bika kwemaminetsi maviri, sefa mushure mekutonhora, uye geza sefa nemvura kusvika wawana 100 mL yefiltrate.Ku10 mL yefiltrate wedzera 1 mL ye dilute sulfuric acid: hapana turbidity inoonekwa.
(4) Zvinhu zvinoenderana-Dissolve 0.20 g yeChenodeoxycholic Acid mumusanganiswa weacetone nemvura (9: 1) kugadzira 10 mL chaiyo, uye shandisa mhinduro iyi semuenzaniso wekugadzirisa.Neparutivi, nyungudutsa 10 mg ye lithocholic acid kune yakaonda-layer chromatography mumusanganiswa weacetone nemvura (9: 1) kuti iite 10 mL chaiyo.Pipeti 2 mL yemhinduro iyi, wedzera musanganiswa weacetone nemvura (9: 1) kuti ugadzire 100 mL chaiyo, uye shandisa mhinduro iyi semhinduro yakajairwa (1).Neparutivi, nyungudutsa 10 mg yeursodeoxycholic acid mumusanganiswa weacetone nemvura (9: 1) kuti uite 100 mL chaiyo, uye shandisa mhinduro iyi seyakajairwa mhinduro (2).Neparutivi, nyungudutsa 10 mg yecholic acid kune yakaonda-layer chromatography mumusanganiswa weacetone nemvura (9: 1) kugadzira 100 mL chaiyo, uye shandisa mhinduro iyi seyakajairwa mhinduro (3).Pipei 1 mL yemhinduro yemuenzaniso, uye wedzera musanganiswa weacetone nemvura (9: 1) kuita 20 mL chaiyo.Pipeti 0.5 mL, 1 mL, 2 mL, 3 mL uye 5 mL yemhinduro iyi, wedzera musanganiswa weacetone nemvura (1) kune imwe neimwe yazvo kuti iite 50 mL chaiyo, uye sarudza mhinduro idzi seyakajairwa mhinduro A, yakajairwa mhinduro. B, yakajairwa mhinduro C, yakajairwa mhinduro D uye yakajairwa mhinduro E, zvichiteerana.Ita bvunzo nemhinduro idzi sekurairwa pasi peThin-layer Chromatography<2.03>.Spot 5 mL imwe neimwe yemhinduro yemuenzaniso, yakajairwa mhinduro (1), (2), (3) uye yakajairwa mhinduro A, B, C, D uye E pandiro ye silica gel ye thin-layer chromatography.Gadzira ndiro nemusanganiswa we 4-methyl-2-pentanone, toluene uye formic acid (16: 6: 1) kune chinhambwe chingangoita masendimita gumi nemashanu, omesa ndiro, uye wowedzera kuoma pa1209C kwemaminitsi makumi matatu.Pakarepo, pfapfaidza zvakaenzana mhinduro yephosphomolybdic acid n-hydrate mu ethanol (95) (1 mu5) pandiro, uye kupisa pa120 ℃ kwemaminetsi maviri kusvika matatu: nzvimbo inoenderana nenzvimbo ine yakajairwa mhinduro (1) haina kusimba kupfuura nzvimbo ine mushonga wakajairwa (1), nzvimbo inoenderana nenzvimbo ine mushonga wakajairika (2) haina kusimba kupfuura nzvimbo ine mushonga wakajairwa (2), uye nzvimbo inoenderana nenzvimbo. neyakajairwa mhinduro (3) haina kusimba kupfuura nzvimbo ine yakajairwa mhinduro
(3).Kana zvichienzaniswa nenzvimbo dzine mhinduro dzakajairwa A, B, C, D uye E, makwapa kunze kwenzvimbo huru uye kunze kwemavara ataurwa pamusoro haana kusimba kupfuura nzvimbo ine yakajairwa mhinduro E, uye huwandu hwese. yavo haisi kupfuura 1.5%.
Kurasikirwa pakuomeswa <2.41> Kwete kupfuura 1.5% (1 g, 105℃,3 maawa).
Zvisaririra pakubatidza <2.44> Kwete kupfuura 0.1% (1 g).
Assay Weresa nemazvo nezve 0.5 g yeChenodeoxycholic Acid, yakamboomeswa, inonyungudika mu40 mL ye ethanol (95) uye 20 mL yemvura, uye titrate <2.50> ine 0.1 mol/L sodium hydroxide VS (potentiometric titration).Ita chisungo chisina chinhu nenzira imwechete, uye ita chero kururamisa kunodiwa.
Imwe mL ye0.1 mol/L sodium hydroxide VS ＝ 39.26 mg yeC24H40O4
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