Citric Acid Anhydrous CAS 77-92-9 Assay 99.5~100.5%

Citric acid [77-92-9].
DEFINITION
Anhydrous Citric Acid ine NLT 99.5% uye NMT 100.5% yeC6H8O7, yakaverengerwa pane anhydrous basis.
KUZIVA
• Infrared Absorption <197K>: Pukuta chinhu kuti chiongororwe pa105℃ kwe2 h.
ASSAY
• Maitiro
Muenzaniso: 0.550 g yeAnhydrous Citric Acid;kunyora uremu nemazvo.
Kuongorora: Dissolve Sample mu 50 mL yemvura.Wedzera 0.5 mL ye phenolphthalein TS.Titrate ine 1 N sodium hydroxide VS.Imwe mL ye1 N sodium hydroxide yakaenzana ne64.03 mg yeC6H8O7.
Maitiro ekugamuchira: 99.5% -100.5% pane anhydrous basis
KUSIRIRA
Inorganic Impurities
• Zvakasara paKubatidza <281>: NMT 0.1%, yakatemwa pa1.0 g
• Masimbi Anorema <231>: NMT 10 ppm
• Sulfate
Standard sulfate solution A: 1.81 mg/mL ye potassium sulfate mu 30% doro.Pakarepo usati washandisa, endesa 10.0 mL yemhinduro iyi kune 1000-mL volumetric flask, dilue ne30% doro kusvika kuhuwandu, uye sanganisa.Mushonga uyu une 10 µg/mL yesulfate.
Standard sulfate solution B: 1.81 mg/mL ye potassium sulfate mumvura.Pakarepo usati washandisa, endesa 10.0 mL yemhinduro iyi kune 1000-mL volumetric flask, dilue nemvura kusvika kuhuwandu, uye sanganisa.Mushonga uyu une 10 µg/mL yesulfate.
Sample stock solution: 66.7 mg/mL ye citric acid
Sample solution: Ku 4.5 mL yeStandard sulfate solution A, wedzera 3 mL yemushonga we barium chloride (1 mu4), zunza, uye rega kumira kweminiti imwe.Ku 2.5 mL yezvakaguma zvamiswa, wedzera 15 mL yeSample stock solution uye 0.5 mL ye5 N acetic acid, wosanganisa.
Mushonga wakajairwa: Gadzirira sekurairwa kwemushonga weSample, kunze kwekushandisa 15 mL yeStandard sulfate solution B pachinzvimbo cheSample stock solution.
Analysis
Samples: Yakajairwa mhinduro uye Sample mhinduro
Maitiro ekugamuchira: Chero turbidity inogadzirwa muSample solution mushure me5 min yakamira haina kukura kupfuura iyo yakagadzirwa muStandard solution (0.015%).
• Limit yeAluminium (painonyorwa seyakagadzirirwa kushandiswa mudialysis)
Standard aruminiyamu solution: Ku 352 mg yealuminium potassium sulfate mu 100-mL volumetric flask, wedzera mashoma mL emvura, swirl kunyunguduka, wedzera 10 mL ye diluted sulfuric acid, diluted nemvura kusvika kuhuwandu, uye sanganisa.Pakarepo usati washandisa, dilue 1.0 mL yemhinduro iyi nemvura kusvika ku100.0 mL.
pH 6.0 acetate buffer: Dissolve 50 g yeammonium acetate mu150 mL yemvura, gadzirisa neglacial acetic acid kusvika pH ye6.0, diluted nemvura kusvika 250 mL, uye sanganisa.
Mushonga wakajairwa: Gadzirira musanganiswa we2.0 mL yeStandard aluminium solution, 10 mL yepH 6.0 acetate buffer, uye 98 mL yemvura.Bvisa musanganiswa uyu sekutsanangurwa kwemushonga weSample, dzikisa zvakasanganiswa zvakatorwa nechloroform kusvika kuhuwandu, uye sanganisa.
Sample solution: Dissolve 20.0 g yeAnhydrous Citric Acid mu100 mL yemvura, wowedzera 10 mL yepH 6.0 acetate buffer.Bvisa mhinduro iyi nezvikamu zvakatevedzana zve 20, 20, uye 10 mL ye 0.5% solution ye8-hydroxyquinoline mu chloroform, uchibatanidza zvinyorwa zvechloroform mu50-mL volumetric flask.Dhiraisai zvakasanganiswa zvakatorwa ne chloroform kusvika kuhuwandu, uye sanganisa.
Mushonga usina chinhu: Gadzirira musanganiswa we10 mL yepH 6.0 acetate buffer uye 100 mL yemvura.Bvisai musanganiswa uyu sekutsanangurwa kweSample solution, bvisa zvakasanganiswa zvakatorwa nechloroform kusvika kuhuwandu, uye sanganisa.
Fluorometric mamiriro
Kuwedzeredza wavelength: 392 nm
Emission wavelength: 518 nm
Analysis
Samples: Yakajairwa mhinduro uye Sample mhinduro
Sarudza iyo fluorescence intensity yeSamples mune fluorometer yakaiswa sekurairwa pasi peFluorometric mamiriro, uchishandisa iyo Blank mhinduro kuseta chiridzwa kusvika zero.
Maitiro ekugamuchira: Iyo fluorescence yeSample solution haipfuure iyo yeStandard solution (0.2 ppm).
Organic Kusachena
• Maitiro: Muganhu weOxalic Acid
Sample stock solution: 200 mg/mL yeAnhydrous Citric Acid mumvura
Sample solution: Ku 4 mL yeSample stock solution wedzera 3 mL yehydrochloric acid uye 1 g yegranular zinc, bika kweminiti imwe, uye bvumira kumira kwemaminetsi maviri.Chinja mashura kuchubhu yekuyedza ine 0.25 mL yephenylhydrazine hydrochloride solution (1 mu100), uye kupisa kusvika kufashaira.Kutonhorera nekukurumidza, endesa kune cylinder yapedza kudzidza, uye wedzera huwandu hwakaenzana hwehydrochloric acid uye 0.25 mL ye potassium ferricyanide solution (1 mu20).Shake, uye bvumira kumira kwemaminitsi makumi matatu.
Standard solution: Gadzirira sezvakarongedzerwa kuSample solution, kunze kwekushandisa 4 mL ye0.10 mg/mL oxalic acid solution, yakaenzana ne0.0714 mg/mL yeanhydrous oxalic acid, panzvimbo yeSample stock solution.[Cherechedza—Gadzirira pamwe chete neSample solution.]
Analysis
Samples: Yakajairwa mhinduro uye Sample mhinduro
Maitiro ekugamuchira: Chero ruvara rwepingi rwakagadzirwa muSample solution haina kusimba kupfuura iyo yakagadzirwa muStandard solution (0.036%).
ZVIYEDZO ZVAKANAKA
• Bacterial Endotoxins Test 85: Huwandu hwemabhakitiriya endotoxins ndehwekuti chinodiwa mune yakakodzera dosage fomu monograph(s) umo Anhydrous Citric Acid inoshandiswa inogona kuzadzikiswa.Iko iyo label inotaura kuti Anhydrous Citric Acid inofanirwa kuiswa pasi pekuenderera mberi nekugadziriswa panguva yekugadzirira mafomu ekubaya jekiseni, chiyero chebhakitiriya endotoxins ndechekuti chinodiwa mune yakakodzera dosage fomu monograph (s) umo Anhydrous Citric Acid inoshandiswa inogona kuve. vakasangana.
• Kujeka Kwemhinduro
[Cherechedza-Mushonga weSample uchafananidzwa neStandard suspension A mumasikati machena maminetsi mashanu mushure mekugadzirwa kweStandard kumiswa A. ]
Hydrazine sulfate solution: 10 mg/mL ye hydrazine sulfate mumvura.Bvumira kumira kwe4 kusvika ku6 h usati washandisa.
Methenamine solution: Kuendesa 2.5 g yemethenamine kune 100-mL glass-stoppered flask, wedzera 25.0 mL yemvura, isa chivharo chegirazi, uye sanganisa kuti unyunguduke.
Primary opalescent suspension: Kuendesa 25.0 mL yeHydrazine sulfate solution kune 25.0 mL yeMethenamine solution mu100-mL glass-stoppered flask.Sanganisa, uye bvumira kumira kwe24 h.[Cherechedza—Kuturika uku kunogadzikana kwemwedzi miviri, kunze kwekunge yachengetwa mugaba regirazi risina kuremara.Iyo yekumisa haifanire kuomerera kune girazi uye inofanira kunge yakanyatso kusanganiswa isati yashandiswa.]
Opalescence standard: Dilute 15.0 mL yePrimary opalescent suspension nemvura kusvika ku1000 mL.[Cherechedza—Kuturika uku hakufanire kushandiswa kupfuura maawa makumi maviri nemana mushure mekugadzirira.]
Standard kuturika A: Dilute 5.0 mL yeOpalescence standard nemvura kusvika ku100 mL.
Standard kuturika B: Dilute 10.0 mL yeOpalescence standard nemvura kusvika ku100 mL.
Sample solution: 200 mg/mL yeAnhydrous Citric Acid mumvura
Analysis
Samples: Standard kuturika A, Standard kuturika B, mvura, uye Sample solution
Tumira chikamu chakakwana cheSample solution kune test tube yegirazi isina ruvara, yakajeka, isina kwayakarerekera ine chigadziko chakafuratira uye dhayamita yemukati ye15-25 mm kuti uwane kudzika kwe40 mm.Saizvozvo chinjisa zvikamu zveStandard suspension A, Standard kuturika B, uye mvura kuti iparadzanise machubhu ekuyedza anoenderana.Enzanisa iyo Sample solution, Standard kuturika A, Standard kuturika B, uye mvura mumasikati machena, uchiona wakatwasuka uchitarisana neruvara rutema (ona Spectrophotometry uye Light-Scattering 851, Visual Comparison).[Cherechedza—Kupatsanurwa kwechiedza kunofanira kunge kwakaita kuti Standard kuturika A kugone kusiyaniswa kubva mumvura, uye kuti Standard kuturika B kunogona kusiyaniswa kubva kune Standard kuturika A. ]
Maitiro ekugamuchira: Sample solution inoratidza kujeka kwakafanana nekwemvura.
• Ruvara rweKugadzirisa
Standard stock solution A: Ferric chloride CS, cobaltous chloride CS, uye dilute hydrochloric acid (10 g/L) (2.4:0.6:7.0)
Standard stock solution B: Ferric chloride CS, cobaltous chloride CS, cupric sulfate CS, uye dilute hydrochloric acid (10 g/L) (2.4:1.0:0.4:6.2)
Standard stock solution C: Ferric chloride CS, cobaltous chloride CS, uye cupric sulfate CS (9.6:0.2:0.2)
[Cherechedza-Gadzirira Magadzirirwo emhinduro pakarepo usati washandisa.]
Standard solution A: Dilute 2.5 mL yeStandard stock solution A ine dilute hydrochloric acid (10 g/L) kusvika pa100 mL.
Standard solution B: Dilute 2.5 mL yeStandard stock solution B ine dilute hydrochloric acid kusvika (10 g/L) 100 mL.
Standard solution C: Dilute 0.75 mL yeStandard stock solution C ine dilute hydrochloric acid (10 g/L) kusvika pa100 mL.
Sample solution: Shandisa Sample solution yakagadzirirwa sekurairwa muyedzo yeClarity of Solution.
Analysis
Samples: Standard solution A, Standard solution B, Standard solution C, mvura, uye Sample solution
Tumira chikamu chakakwana cheSample solution kune test tube yegirazi isina ruvara, yakajeka, isina kwayakarerekera ine chigadziko chakafuratira uye dhayamita yemukati ye15-25 mm kuti uwane kudzika kwe40 mm.Saizvozvo fambisa zvikamu zve Standard solution A, Yakajairwa mhinduro B, Yakajairwa mhinduro C, uye mvura kuti iparadzanise inoenderana machubhu ebvunzo.Enzanisa Sample solution, Standard solution A, Standard solution B, Standard solution C, uye mvura mumasikati machena, uchiona wakamira uchitarisana nemuchena (ona Spectrophotometry uye Light-Scattering 851, Visual Comparison).
Maitiro ekugamuchira: Iyo Sample solution haina kunyanyisa ruvara kupfuura Standard solution A, B, kana C, kana mvura.
• Zviri nyore Carbonizable Substances
Muenzaniso: 1.0 g yeupfu Anhydrous Citric Acid
Kuongorora: Tumira Sample kune 22- × 175-mm test chubhu yakambogezwa ne 10 mL ye sulfuric acid uye yakabvumirwa kudonha kwemaminitsi gumi.Wedzera 10 mL ye sulfuric acid, simbisa kusvikira mhinduro yapera, uye inyura mumvura yekugeza pa 90 ± 1 ye 60 ± 0.5 min, kuchengetedza chiyero cheasidhi pasi pezinga remvura munguva yose.Tonhodza chubhu mumvura inoyerera, uye endesa asidhi kune imwe ruvara-yekuenzanisa chubhu.
Maitiro ekugamuchira: Rudzi rweasidhi haruna kusviba kupfuura yevhoriyamu yakafanana yeMatching Fluid K (ona Ruvara uye Achromicity 631) muchubhu inofananidzwa, machubhu achicherechedzwa akamira achitarisana neshure chena.
• Sterility Miedzo 71: Kana iyo label inotaura kuti Anhydrous Citric Acid haina mbereko, inosangana nezvinodiwa zveSterility Test 71 mune yakakodzera dosage fomu monograph(s) umo Anhydrous Citric Acid inoshandiswa.
• Kusarudza Mvura, Nzira I 921: NMT 1.0%
ZVIMWE ZVINODIWA
• Kurongedza uye Kuchengeta: Chengetedza mumidziyo yakaoma.Hapana zvichemo zvekuchengetedza zvakatsanangurwa.
• Kunyora: Painoitirwa kushandiswa mukugadzirisa dialysis, yakanyorwa kudaro.Iko Anhydrous Citric Acid inofanirwa kuiswa pasi pekuwedzera kugadziridzwa panguva yekugadzirwa kweanojairwa dosage mafomu kuti ave nechokwadi chinogamuchirika mazinga emabhakitiriya endotoxins, yakanyorwa kudaro.Iko Anhydrous Citric Acid isina mbereko, yakanyorwa kudaro.
• USP Reference Standards 11
USP Citric Acid RS Dzvanya kuti uone Chimiro
USP Endotoxin RS