Histamine Dihydrochloride CAS 56-92-8 Assay 98.5~101.0% (Titration) Factory

Histamine Dihydrochloride (CAS: 56-92-8) EP9.0 VhoriyamuⅡTest Method
DEFINITION
Histamine dihydrochloride ine isingasviki 98.5 muzana uye isingapfuure yakaenzana ne101.0 muzana ye2-(1H-imidazol-4-yl) ethan-1-amine dihydrochloride, yakaverengerwa ichitaura nezvechinhu chakaomeswa.
VANHU
Ichena kana inenge chena, crystalline poda kana makristasi asina ruvara, hygroscopic, yakanyanya kunyungudika mumvura, inonyungudika mudoro.
KUZIVA
Kuzivikanwa kwekutanga: A, D.
Chechipiri kuzivikanwa: B, C, D
A. Ongorora ne infrared absorption spectrophotometry (2.2.24), uchienzanisa ne spectrum inowanikwa ne histamine dihydrochloride CRS.Ongorora semadhisiki akagadzirirwa uchishandisa 1 mg yezvinhu.
B. Ongorora machromatograms akawanikwa mukuedzwa kwehistidine.Nzvimbo huru muchromatogram yakawanikwa nebvunzo mhinduro (b) yakafanana pachinzvimbo, ruvara uye saizi kune yakakosha nzvimbo muchromatogram yakawanikwa nereferensi mhinduro (a).
C. Dissolve 0.1 g mu7 mL yemvura R wowedzera 3 mL ye200 g/L solution ye sodium hydroxide R. Dissolve 50mg ye sulfanilic acid R mumusanganiswa we 0.1 mL yehydrochloric acid R uye 10 mL yemvura R uye wedzera 0.1 mL ye sodium nitrite solution R. Wedzera mhinduro yechipiri kune yekutanga uye sanganisa.Ruvara rutsvuku runogadzirwa.
D. Inopa kuita (a) kwekloridi (2.3.1).
ZVIYEDZO
Solution S. Dissolve 0.5g mumvura isina carbon dioxide-isina R yakagadzirwa kubva mumvura yakasvibiswa R uye inonatsa kusvika ku10 mL neine solvent yakafanana.
Kuonekwa kwemhinduro.Solution S yakajeka (2.2.1) uye haina kunyanya kupenya kupfuura referensi mhinduro Y7 (2.2.2, Method II).
pH (2.2.3).Iyo pH yemhinduro S ndeye 2.85 kusvika 3.60.
Histidine.Ongorora neyakaonda-layer chromatography (2.2.27), uchishandisa TLC silica gel G ndiro R.
Muedzo mhinduro (a).Dissolve 0.5g yechinhu chinofanirwa kuongororwa mumvura R uye diluted kusvika ku10 mL neine solvent yakafanana.
Mhinduro yekuedza (b).Dilute 2 mL yebvunzo mhinduro (a) kusvika 10 mL nemvura R.
Reference solution (a).Dissolve 0.1 g ye histamine dihydrochloride CRS mumvura R uye dilute kusvika ku10 mL neine solvent yakafanana.
Reference solution (b).Dissolve 50 mg ye histidine monohydrochloride R mumvura R uye shandura kusvika ku100 mL neine solvent yakafanana.
Reference solution (c).Sanganisa 1 mL yebvunzo mhinduro (a) uye 1 mL yereferensi mhinduro (b).
Isa kuplate 1 μL yemhinduro yebvunzo (a), 1 μL yemhinduro yebvunzo (b), 1 μL yereferensi mhinduro (a), 1 μL yereferensi mhinduro (b) uye 2 μL yereferensi mhinduro (c).Gadzira pamusoro penzira ye15cm uchishandisa musanganiswa we5volumes ye concentrated ammonia R, 20 mavhoriyamu emvura R uye 75 mavhoriyamu eacetonitrile R. Pukuta ndiro mumhepo yazvino.Dzokorora kusimudzira munzira imwechete, omesa ndiro mumhepo yemhepo uye pfapfaidza ine ninhydrin mhinduro R1.Pisa ndiro pa 110 ° C kwemaminitsi gumi.Chero nzvimbo inoenderana nehistidine muchromatogram inowanikwa nebvunzo mhinduro (a) haina kusimba kupfuura nzvimbo iri muchromatogram yakawanikwa nereferensi mhinduro (b) (1 muzana).Muedzo haushande kunze kwekunge chromatogram yakawanikwa ine referensi mhinduro (c) inoratidza 2 nzvimbo dzakapatsanurwa zvakajeka.
Sulfates (2.4.13).3mL yemushonga S yakanyungudutswa kusvika pa15 mL nemvura yakanyungudutswa R inopindirana nemuyedzo wemuganho wesarufa (0.1 muzana).
Kurasikirwa pakuomeswa (2.2.32).Kwete kupfuura 0.5 muzana, yakatemwa pa 0.20 g nekuomesa muovheni pa105 ° C.
Sulfated ash (2.4.14).Kwete kupfuura 0.1 muzana, yakatarwa pa 0.5 g.
ASSAY
Dissolve 0.080 g mumusanganiswa we 5.0 mL we0.01 M hydrochloric acid uye 50 mL yedoro R. Ita potentiometric titration (2.2.20), uchishandisa0.1 M sodium hydroxide.Verenga vhoriyamu yakawedzerwa pakati pekutanga neyechitatu mapoinzi einflexion.
1mL ye0.1 M sodium hydroxide yakaenzana ne9.203 mg yeC5H11Cl2N3.
STORAGE
Chengetedza mumudziyo usina mhepo, wakachengetedzwa kubva pachiedza.