L-Cysteine ​​Hydrochloride Monohydrate CAS 7048-04-6 Assay 98.5~101.0% (Titration on Dried Basis) Factory

L-Cysteine ​​Hydrochloride Monohydrate (CAS: 7048-04-6) AJI97 Test Method
Kuzivikanwa: Enzanisa infrared absorption spectrum yemuenzaniso neiyo yechiyero ne potassium bromide disc nzira.
Kutenderera Kwakananga [α]D20: Sample yakaomeswa, C=8, 1mol/L HCl
State of Solution (Transmittance): 1.0g mu10ml yeH2O, spectrophotometer, 430nm, 10mm sero ukobvu.
Chloride (Cl): 350mg, B-2
Ammonium (NH4): A-1
Sulfate (SO4): 1.2g, (1), ref: 0.50ml ye0.005mol/L H2SO4
Simbi (Fe): 1.5g, ref: 1.5ml yeIron Std.(0.01mg/ml)
Heavy Metals (Pb): 2.0g, (1), pH=7, ref: 2.0ml yePb Std.(0.01mg/ml)
Arsenic (As2O3): 2.0g, (1), ref: 2.0ml yeAs2O3 Std.
Mamwe Amino Acids: Test Sample: 50μg, B-6-a, control;L-Cys·HCl·H2O 0.25μg
Kurasikirwa Pakuomesa: Muvacuum, P2O5, pakamuri tembiricha kwemaawa makumi maviri.
Residue paIgnition (Sulfated): AJI Muedzo 13
Chiyero: Muenzaniso wakaomeswa, 250mg, 0.05mol/L I2 1ml=15.762mg C3H7NO2S·HCl
pH Muedzo: 1.0g mu100ml yeH2O

L-Cysteine ​​Hydrochloride Monohydrate (CAS: 7048-04-6) USP35 Test Method
DEFINITION
Cysteine ​​Hydrochloride inonzi L-Cysteine ​​Hydrochloride Monohydrate uye ine NLT 98.5% uye NMT 101.5% yeL-Cysteine ​​Hydrochloride (C3H7NO2S·HCl), yakaverengerwa pakaomeswa.
KUZIVA
A. INFRARED AVORPTION <197K>
ASSAY
PROCEDURE
Muenzaniso: 250 mg yeCysteine ​​Hydrochloride
Blank: Enderera sekurairwa muOngororo pasina Sample.
Titrimetric system
(Ona Titrimetry <541>)
Mode: Residual titration
Titrant: 0.1 N ayodhini VS
Back titrant: 0.1 N sodium thiosulfate VS
Kuonekwa kwekupedzisira: Kuona
Kuongorora: Tumira Sample kune iyo iodine flask.Wedzera 20.0 mL yemvura uye 4 g ye potassium iodide, uye sanganisa.Tonhodza mushonga mukugeza echando, woisa 5 mL ye3 N hydrochloric acid uye 25.0 mL ye0.1 N iodine VS.Isai chivharo, uye bvumira kumira murima kwemaminitsi makumi maviri.Titrate iyo iodine yakawandisa neiyo Back titrant.Wedzera 3 mL yenyeredzi ch TS sezvo magumo ari kusvika.Nechimiro chekutsunga kusina.Verenga chikamu che cysteine ​​hydrochloride (C3H7NO2S·HCl) muSample yakatorwa:
Mhedzisiro = {[(VB − VS) × N × F]/W} × 100
VB = Back titrant vhoriyamu inopedzwa neiyo Blank (mL)
VS = Back titrant vhoriyamu inopedzwa neSample (mL)
N = hunhu chaihwo hweiyo Back titrant (mEq/mL)
F= equivalency factor, 157.6 mg/mEq
W = uremu hwemuenzaniso (mg)
Maitiro ekugamuchira: 98.5% ~ 101.5% pane yakaomeswa hwaro
KUSIRIRA
ZVINOSARA PAKABATIDZA <281>: NMT 0.4%
CHLORIDE UYE SULFATE, Sulfate <221>
Mushonga wakakwana: 0.10 mL ye0.020 N sulfuric acid
Muenzaniso: 0.33 g yeCysteine ​​Hydrochloride
Maitiro ekugamuchira: NMT 0.03%
IRON <241>: NMT 30ppm
METALS ZVIKURU, Nzira I <231>: NMT 15ppm
RELATED COMPOUNDS
N-Ethylmaleimide mhinduro: 40 mg/mL yeN-ethylmaleimide mudoro
Standard stock solution: Dissolve 20 mg ye USP L-Cysteine ​​Hydrochloride RS mu 10.0 mL yemvura.Wedzera 10.0 mL yeN-Ethylmaleimide mhinduro, uye sanganisa.Bvumira mhinduro kumira kwemaminitsi mashanu usati washandisa.
Yakajairwa mhinduro: 0.05 mg/mL kubva kuStandard stock solution mumvura.[ CHERECHEDZA-Iyi mhinduro ine denderedzwa yakaenzana ne0.5% yeiyo yeSample solution.]
Kukodzera kweSystem solution: Kuendesa 10 mg yeUSP L-Tyrosine RS uye 10 mL yeStandard stock solution kune 25-mL volumetric flask.Dilute nemvura kusvika kuhuwandu.
Sample stock solution: Shandura 0.2 g yeCysteine ​​Hydrochloride kune 10-mL volumetric flask, nyungudusa, uye wedzera nemvura kusvika kuhuwandu.
Sample solution: Ku 5.0 mL yeSample stock solution wedzera 5.0 mL yeN-Ethylmaleimide solution, wosanganisa.Bvumira mhinduro kumira kwemaminitsi mashanu usati washandisa.
Chromatographic system (Ona Chromatography <621>, Thin-Layer Chromatography.)
Modhi: TLC
Adsorbent: 0.25-mm layer yechromatographic silica gel musanganiswa
Kushandiswa kwevhoriyamu: 5μL
Kugadzira solvent system: Butyl doro, glacial acetic acid, uye mvura (3: 1: 1)
Spray reagent: 2 mg/mL yeninhydrin mumusanganiswa webutyl doro uye 2N acetic acid (95:5)
Kukodzera kwehurongwa
Zvinodiwa zvekukodzera: Iyo chromatogram yeSystem yekukodzera mhinduro inoratidza maviri akapatsanurwa mavara.
Analysis
Samples: Yakajairwa mhinduro, System kukodzera mhinduro, uye Sample mhinduro.
Wapedza kuomesa ndiro, pfapfaidza neSpray reagent, wopisa pakati pe100° ne105° kwemaminetsi gumi nemashanu.Ongorora ndiro pasi pechiedza chichena.
Maitiro ekugamuchira: Chero nzvimbo yechipiri yeSample solution haina kukura kana kusimba kupfuura nzvimbo huru yeStandard solution.
Kusachena kwemunhu: NMT 0.5%
Yese tsvina: NMT 2.0%
ZVIYEDZO ZVAKANAKA
OPTICAL kutenderera, Chaiyo Rotation <781S>
Sample solution: 80 mg/mL mu6 N hydrochloric acid
Maitiro ekugamuchira: + 5.7 ° kusvika + 6.8 °
LOSSON DRYING <731>: Dry sampuli pakushisa kwekamuri pakumanikidzwa kusingapfuuri 5 mm ye mer cury kwe24 h: inorasikirwa ne8.0% ~ 12.0% yehuremu hwayo.
ZVIMWE ZVINODIWA
PACKAGE AND STORAGE: Chengetedza mumidziyo yakavharwa zvakanaka.
USP REFERENCE MITIRO <11>
USP L-Cysteine ​​Hydrochloride RS
USP L-Tyrosine RS