L-Glutamine CAS 56-85-9 (H-Gln-OH) Chiyero 99.0 ~ 101.0% Factory High Quality

Japanese PharmacopoeiaReference Standards
L-Glutamine [56-85-9]
L-Glutamine, kana yakaomeswa, ine kwete pasi pe99.0% uye kwete kupfuura 101.0% yeL-Glutamine (C5H10N2O3).
Tsanangudzo L-Glutamine inoitika sechena, makristasi kana crystalline poda.Iine zvishoma hunhu kuravira
Inonyungudika zvakasununguka muformic acid, inonyungudika mumvura, uye isinganyungudika mu ethanol (99.5)
Kuzivikanwa Sarudza iyo infrared absorption spectrum yeL-Glutamine sezvainotungamirwa mu potassium bromide disk nzira pasi peInfrared Spectrophotometry <2.25>, uye enzanisa spectrum neReferensi Spectrum: zvose zvinoratidzira zvinoratidza kusimba kwakafanana kwekunyura panhamba imwechete yemafungu.
Optical Rotation <2.49> [a]20D:+6.3° to +7.3° Weresa nemazvo anenge 2 g yeL-Glutamine, yakamboomeswa, wedzera 45mL yemvura, inodziya kusvika 40℃ kunyungudika, uye mushure mekutonhora, wedzera mvura kugadzira. chaizvo 50 mL.Sarudza kutenderera kwemaziso kweiyi mhinduro mune 100-mm sero, mukati memaminitsi makumi matanhatu.
pH <2.54> Iyo pH yemhinduro yakagadzirwa nekunyungudutsa 1.0 g yeL-Glutamine mu50 mL yemvura iri pakati pe4.5 ne6.0.
Kuchena
(1) Kujeka uye ruvara rwemhinduro - Mhinduro inowanikwa nekunyungudutsa 0.5 g yeL-Glutamine mu 20 mL yemvura yakajeka uye isina ruvara.
(2) Chloride <1.03> -Ita bvunzo ne0.5 g yeL-Glutamine.Gadzirira mhinduro yekudzora ne 0.30 mL ye0.01 mol/L hydrochloric acid VS (kwete kupfuura 0.021%).
(3) Sulfate <1.14>—Ita bvunzo ne0.6 g yeL-Glutamine.Gadzirira mhinduro yekudzora ne 0.35 mL ye0.005 mol/L sulfuric acid VS (kwete kupfuura 0.028z).
(4) Ammonium <1.02>—Ita bvunzo ne0.10 g yeL-Glutamine, uchishandisa distillation pasi pekumanikidzwa kwakaderedzwa.Gadzirira mhinduro yekudzora ne10.0 mL yeStandard Ammonium Solution.Tembiricha yekugezera yemvura ndeye45 ℃ (kwete kupfuura 0.1%).
(5) Heavy Metals <1.07>-Ramba ne 1.0 g yeL-Glutamine maererano neMethod 1, uye ita muedzo.Gadzirira mhinduro yekudzora ne 1.0 mL yeStandard Lead Solution (kwete kupfuura 10 ppm).
(6) Iron <1.10>-Gadzirira mhinduro yekuedza ne 1.0 g yeL-Glutamine maererano neMethod 1, uye ita muedzo maererano neMethod A. Gadzirira sarudzo yekutonga ne1.0 mL yeStandard Iron Solution (kwete kupfuura 10 ppm)
(7) Zvinhu Zvine hukama-Dissolve 0.10 g yeL-Glutaminein 10 mL yemvura, uye shandisa iyi mhinduro semuyedzo wemhinduro.Pipeti 1 mL yemhinduro yemuenzaniso, wedzera mvura kuita 10 mL chaiyo.Pipeti 1 mL yemhinduro iyi, wedzera mvura kuita 50 mL chaiyo, uye shandisa mhinduro iyi seyakajairwa mhinduro.Ita bvunzo nemhinduro idzi sekurairwa pasi peThin-layer Chromatography <2.03>.Spot 5mL imwe neimwe yemhinduro yemuenzaniso uye yakajairwa mhinduro pandiro yesilicagel kune yakatetepa-layer chromatography.Wobva wagadzira nemusanganiswa we1-butanol, mvura uye acetic acid (100) (3:1:1) kusvika kureba ingangoita masendimita gumi, woomesa ndiro yacho pa80℃ kwemaminitsi makumi matatu.Pfaya zvakaenzana mhinduro yeninhydrin mumusanganiswa wemethanol uye acetic acid (100) (97:3) (1 mu100) pandiro, uye kupisa pa80 ℃ kwemaminetsi gumi: nzvimbo kunze kwenzvimbo huru yakawanikwa ne mhinduro yemuenzaniso haina kusimba kupfuura nzvimbo inowanikwa neyakajairwa mhinduro.
Kurasikirwa paKuomesa <2.41> Kwete kupfuura 0.3% (1 g, 105℃, maawa matatu)
Residue paIgnition <2.44> Kwete kupfuura 0.10%(1 g).
Assay Weresa nemazvo nezve 0.15 g yeL-Glutamine, yakamboomeswa, inonyungudika mu3 mL yefomic acid, wedzera 50 mL yeacetic acid (100), uye titrate <2.50> ine 0.1 mol/L perchloric acid VS (potentiometric titration).Ita chisungo chisina chinhu nenzira imwechete, uye ita chero kururamisa kunodiwa.
Imwe mL ye0.1 mol/L perchloric acid VS＝14.61 mg yeC5H10N2O3
Midziyo nemidziyo yekuchengetera—Midziyo yakasimba.

USP Reference Standards <11>-USP Glutamine RS.
Identification, Infrared Absorption <197K>.
Kutenderera kwakananga <781S>: pakati pe +6.3 ° uye +7.3 °, yakatarwa pa20 ℃
Test Solution- Mubhokisi rakakodzera, nyatsogadzira mhinduro inenge 40mg per ml mumvura inenge 40 ℃.Kutonhodza uye wedzera nemvura kusvika kuhuwandu usati washandisa.
Kurasikirwa paKuomesa <731>-Dry it pa 105 ℃ kwemaawa matatu;inorasikirwa kwete kupfuura 0.20% yehuremu hwayo.
Residue paIgnition <281>Kwete kupfuura 0.30%
Chloride <221>-A 0.7-g chikamu chinoratidza hapana chloride kupfuura inoenderana ne0.50ml ye0.020 N hydrochloric acid: kwete kupfuura 0.05% inowanikwa.
Sulfate <221>-A 0.8-g chikamu hachiratidzi sulfate kupfuura inoenderana ne0.25ml ye0.020 N sulfuric acid: kwete kupfuura 0.03% inowanikwa.
Chromatographic Purity
Adsorbent: 0.25-mm layer yechromatographic silica gel musanganiswa.
Test Solution: 10mg per ml, mumvura
Standard Solution-Gadzirira mhinduro yeUSP Glutamine RS mumvura ine huwandu hunozivikanwa hunosvika 0.05mg per ml.
Chikumbiro Vhoriyamu: 5μL
Kugadzira Solvent System: musanganiswa webutyl doro, mvura, uye glacial acetic acid (3: 1: 1).
Spray Reagent-Dissolve 0.2g yeninhydrin mu100ml yemusanganiswa webutyl doro uye 2 N acetic acid (95:5)
Procedure-Endererai sekurairwa kwaita thin-layer chromatography pasi peChromatography <621>, wobva waomesa ndiro pa80℃ kwemaminitsi makumi matatu.Pfaya ndiro ine spray reagent, kupisa pa80 ℃ kwemaminetsi gumi, uye ongorora pasi pechiedza chichena: hapana chechipiri nzvimbo muchromatogram yakawanikwa kubva muyedzo mhinduro yakakura kana kusimba kupfuura nzvimbo huru muchromatogram yakawanikwa kubva kune standrad solution. (0.5%)
Organic Volatile Impurities, Nzira I <467>: Sangana nezvinodiwa.
Asssy-Shandura pamusoro pe 150mg yeGlutamine, yakanyatsorema, kune 125ml flask, dissolbe mu 3ml yefomic acid uye 50ml ye glacial acetic acid, uye titrate ne 0.1 N perchloric acid VS inogadzirisa kuguma potentiometrically.Ita chisungo chisina chinhu, uye ita chero gadziriso inodiwa (ona Titrimetry<541>).Imwe neimwe ml ye0.1 N perchloric acid yakaenzana ne14.615 mg yeC5H10N2O3