L-Leucine Methyl Ester Hydrochloride CAS 7517-19-3 (H-Leu-OMe·HCl) Chiyero >99.0% Factory

1. Chitarisiko
Tora huwandu hwakakodzera hwechigadzirwa ichi, uise papepa chena rakatsetseka uye uparadzire 5 cm2 uye 5 mm gobvu.Zvione munzvimbo inopenya.
2. Kuchena (HPLC)
2.1 Chiridzwa
Yakakwira performance liquid chromatography, PDA detector.
Electronic analytical balance
2.2 Reagents
Acetonitrile (chromatographic giredhi), TFA (chromatographic giredhi)
2.3 Chromatographic mamiriro
2.3.1 Koramu: YMC-ODS-AM, 5 μl, 150 x 4.6 mm
2.3.2
Kuonekwa wavelength: INC220nm
Kuyerera kweyero: 1.0mL/min
Jekiseni vhoriyamu:10 μL (yekureva)
Diluent: acetonitrile
Nguva yekumhanya: maminitsi makumi maviri
2.4 Kugadzirira kwechikamu chenhare
Nharembozha chikamu A (0.1% trifluoroacetic acid mvura): kunyatsotora 2.0ml TFA, Dilute kusvika 2000ml nemvura, mix uye degas;
Nharembozha chikamu B (0.1% trifluoroacetic acid acetonitrile): kunyatsotora 2.0ml TFA, Dilute kusvika 2000ml neacetonitrile, mix uye degas;
2.5 Instrumental parameters
Nguva (miniti) A% B%
0.00 90 10
12.00 10 90
15.00 10 90
15.01 90 10
20.00 90 10
2.6 Kugadzirira kwemuenzaniso mhinduro
Weresa uye nyungudutsa 0.1g sampu ine acetonitrile, dilute kusvika ku100ml, zunza zvakanaka kuti ushandiswe, kana iyo yakafanana.Gadzirira sampuli mbiri dzakafanana.
2.7 Sample Kutsunga
Ongorora sampuli maererano neanotevera sampling maitiro:
Kupfuura jekiseni 1 remhinduro isina chinhu
1 tsono muenzaniso mhinduro 1#
1 tsono muenzaniso mhinduro 2#
2.8 Mhedzisiro Kuverenga
2.8.1 Peak area normalization nzira yakashandiswa kuverenga kuchena kweHPLC nekubvisa nzvimbo isina chinhu.
2.8.2 Iyo hama inoreva kutsauka kwekuchena kwetsono mbiri haifaniri kudarika 1%
2.8.3 Kana mhedzisiro yemajekiseni ese ari maviri akazadzisa maitiro ekugamuchirwa, avhareji yekuchena inotorwa semhedzisiro yekupedzisira.
3, Melting point -- RY-1 melting point chiridzwa
4. Kurasikirwa pakuomesa bvunzo nzira
4.1 Zviridzwa:
Electric thermostatic drying oven, 1/10,000 chiyero.
4.2 Maitiro:
Mubhodhoro rehuremu rakafuratira rine huremu hunoramba huchiwedzera uye kuoma-kuoma kwevhu rakavhara muromo, kuyera 1 gramu (yakarurama kusvika 0.0001 gramu) yemuenzaniso.Sample inofanira kuparadzirwa zvakaenzana pazasi pebhodhoro rekuyeresa nehupamhi husingapfuure 10mm, isa muovheni yekuomesera yemagetsi, yakaoma pa105 ~ 110 ℃ kwemaawa matatu, wozoenda muimba yekuomesa kuti utonhorere. kamuri tembiricha yekuyera.
Kuverenga: Kurasikirwa pakuomeswa %= (M1-M2) ÷M×100
Ikoko: M1: uremu hwemuenzaniso uye bhodhoro rekuyeresa risati raoma, magiramu
M2: uremu hwemuenzaniso uye bhodhoro rekuyeresa mushure mekuoma, mumagiramu
M: Sample uremu, magiramu
5. Kutenderera chaiko
-- Kutenderera chaiko kunoyerwa zvinoenderana neGB/T613-1988
Kugadzirira kwemuenzaniso: 1.0000g sampuli yakayerwa zvakarurama uye yakaendeswa kune yakachena uye yakaoma 50ml volumetric bhodhoro, 20ml mvura yakawedzerwa, bhodhoro rakavharwa uye rakazununguswa kuti riparare, uye rinoderedzwa kusvika pachiyero nemvura.
Muedzo: Gadzirisa zero yegyroscope isati yaedzwa, wobva waisa chubhu yebvunzo nemhinduro yemuenzaniso, rekodha Angle yekutenderera, uye verenga kutenderera chaiko kwesample neinotevera formula.
[а]D20 = (r×50) ÷ (L×W)
[а]D20: Yakananga optical kutenderera pa20 ℃ yemuenzaniso mhinduro
r: Kutenderera kweOptical kunoonekwa pa20 ℃ kune mhinduro yemuenzaniso
50: Vhoriyamu yeyakagadzirirwa sampuli mhinduro (ml)
w: uremu hwemuenzaniso (g)
L: Optical kutenderera chubhu kureba (dm)