L-Proline CAS 147-85-3 (H-Pro-OH) Chiyero 98.5~101.0% Factory High Quality

L-Proline (H-Pro-OH) (CAS: 147-85-3) AJI 97 Test Method
Kuzivikanwa: Enzanisa infrared absorption spectrum yemuenzaniso neiyo yechiyero ne potassium bromide disc nzira.
Kutenderera kwakananga [α]20/D: Yakaomeswa Sample, C=4, H2O
State of Solution (Transmittance): 1.0g mu10ml yeH2O, spectrophotometer, 430nm, 10mm sero ukobvu.
Chloride (Cl): 0.7g, A-1, ref: 0.40ml ye0.01mol/L HCl
Ammonium (NH4): B-1
Sulfate (SO4): 1.2g, (1), ref: 0.50ml ye0.005mol/L H2SO4
Simbi (Fe): 1.5g, ref: 1.5ml yeIron Std.(0.01mg/ml)
Heavy Metals (Pb): 2.0g, (1), ref: 2.0ml yePb Std.(0.01mg/ml)
Arsenic (As2O3): 2.0g, (1), ref: 2.0ml yeAs2O3 Std.
Mamwe Amino Acids: Test Sample: 30μg, B-6-a
Kurasikirwa paKuomesa: pa105 ℃ kwemaawa matatu.
Chiyero: Sample yakaomeswa, 120mg, (1), 3ml yefomic acid, 50ml yeglacial acetic acid, 0.1mol/L HCLO4 1ml = 11.513mg C5H9NO2
pH Muedzo: 1.0g mu10ml yeH2O

L-Proline (H-Pro-OH) (CAS: 147-85-3) USP35 Test Method
DEFINITION
Proline ine NLT 98.5% uye NMT 101.5% yeL-Proline (C5H9NO2), yakaverengerwa payakaomeswa.
KUZIVA
A. INFRARED AVORPTION <197K>
ASSAY
PROCEDURE
Muenzaniso: 110 mg yeProline
Chisina chinhu: Sanganisa 3 mL yeformic acid uye 50 mL yeglacial acetic acid.
Titrimetric system
(Ona Titrimetry <541>)
Mode: Direct titration
Titrant: 0.1 N perchloric acid VS
Endpoint kuona: Potentiometric
Ongororo: Dissolve Sample mu3 mL yeformic acid uye 50mL yeglacial acetic acid.Titrate pamwe neTitrant.Ita kusarudzika.
Verenga chikamu cheProline (C5H9NO2) muSample yakatorwa:
Mhedzisiro = {[(VS-VB) x N x F]/W} x100
VS = vhoriyamu yeTitrant inopedzwa neSample (mL)
VB = vhoriyamu yeTitrant inopedzwa neiyo Blank (mL)
N = hunhu chaihwo hweTitrant (mEq/mL)
F= equivalency factor, 115.1 mg/mEq
W = uremu hwemuenzaniso (mg)
Maitiro ekugamuchira: 98.5% ~ 101.5% pane yakaomeswa hwaro
KUSIRIRA
ZVINOSARA PAKABATIDZA <281>: NMT 0.4%
CHLORIDE NESULFATE, Chloride <221>
Mushonga wakakwana: 0.50mL ye0.020 N hydrochloric acid
Muenzaniso: 0.73g yeProline
Maitiro ekugamuchira: NMT 0.05%
CHLORIDE UYE SULFATE, Sulfate <221>
Mushonga wakakwana: 0.10mL ye0.020 N sulfuric acid
Muenzaniso: 0.33g yeProline
Maitiro ekugamuchira: NMT 0.03%
IRON <241>: NMT 30ppm
METALS ZVIKURU, Nzira I <231>: NMT 15ppm
RELATED COMPOUNDS
Sistimu yakakodzera mhinduro: 0.4mg/mL imwe neimwe yeUSP L-Proline RS uye USP L-Threonine RS mu0.1 N hydrochloric acid
Mhinduro yakajairwa: 0.05mg/mL yeUSP L-Proline RS mu 0.1 N hydrochloric acid.[Cherechedza-Iyi mhinduro ine kusungwa kwakaenzana ne0.5% yeiyo yeSample solution.]
Sample solution: 10mg/mL yeProline mu 0.1 N hydrochloric acid
Chromatographic system
(Ona Chromatography <621>, Thin-Layer Chromatography.)
Modhi: TLC
Adsorbent: 0.25-mm layer yechromatographic silica gel musanganiswa
Kushandiswa kwevhoriyamu: 5μL
Kugadzira solvent system: Butyl doro, glacial acetic acid, uye mvura (3: 1: 1)
Spray reagent: 2 mg/mL yeninhydrin mumusanganiswa webutyl doro uye 2N acetic acid (95:5)
Kukodzera kwehurongwa
Zvinodiwa zvekukodzera: Iyo chromatogram yeSystem yekukodzera mhinduro inoratidza maviri akapatsanurwa mavara.
Analysis
Samples: Kukodzera kweSistimu mhinduro, Yakajairwa mhinduro, uye Sample mhinduro.
Wapedza kuomesa ndiro, pfapfaidza neSpray reagent, wopisa pakati pe100° ne105° kwemaminetsi gumi nemashanu.Ongorora ndiro pasi pechiedza chichena.
Maitiro ekugamuchira: Chero nzvimbo yechipiri yeSample solution haina kukura kana kusimba kupfuura nzvimbo huru yeStandard solution.
Kusachena kwemunhu: NMT 0.5%
Yese tsvina: NMT 2.0%
ZVIYEDZO ZVAKANAKA
OPTICAL kutenderera, Chaiyo Rotation <781S>
Sample solution: 40 mg/mL mumvura
Maitiro ekugamuchira: -84.3 ° kusvika -86.3 °
LOSSON DRYING <731>: Dry sample pa105℃ kwe3 h: inorasikirwa neNMT 0.4% yehuremu hwayo.
ZVIMWE ZVINODIWA
PACKAGE AND STORAGE: Chengetedza mumidziyo yakavharwa zvakanaka.
USP REFERENCE MITIRO <11>
USP L-Proline RS
USP L-Threonine RS