L-Tryptophan CAS 73-22-3 (H-Trp-OH) Chiyero 98.5 ~ 101.5% Factory High Quality

L-Tryptophan (H-Trp-OH) (CAS: 73-22-3) AJI 97 Test Method
Kuzivikanwa: Enzanisa infrared absorption spectrum yemuenzaniso neiyo yechiyero ne potassium bromide disc nzira.
Chaiyo Kutenderera [α]20/D: Yakaomeswa Sample, C=1, H2O nyungudika nekudziya
State of Solution (Transmittance): 0.5g mu20ml ye2 mol/L HCl, spectrophotometer, 430nm, 10mm sero ukobvu.
Chloride (Cl): 0.7g, A-1, ref: 0.40ml ye0.01mol/L HCl
Ammonium (NH4): B-2
Sulfate (SO4): 1.2g, (1), ref: 0.50ml ye0.005mol/L H2SO4
Simbi (Fe): 0.75g, (2), ref: 1.5ml yeIron Std.(0.01mg/ml)
Heavy Metals (Pb): 2.0g, (4), ref: 2.0ml yePb Std.(0.01mg/ml)
Arsenic (As2O3): 2.0g, (1), ref: 2.0ml yeAs2O3 Std.
Mamwe maAmino Acids: H2O, Dissolve nekudziya (40 ℃) Test Sample: 50μg, A-6-a, control: L-Trp 0.25μg
Kurasikirwa paKuomesa: pa105 ℃ kwemaawa matatu.
Residue paIgnition (Sulfated): AJI Muedzo 13
Chiyero: Muenzaniso wakaomeswa, 200mg, (1), 3ml yefomic acid, 50ml yeglacial acetic acid, 0.1mol/L HCLO4 1ml = 20.423mg C11H12N2O2
pH Muedzo: 1.0g mu100ml yeH2O

L-Tryptophan (H-Trp-OH) (CAS: 73-22-3) USP35 Test Method
DEFINITION
Tryptophan ine NLT 98.5% uye NMT 101.5% yeC11H12N2O2, seL-Tryptophan, yakaverengerwa pane yakaomeswa.
KUZIVA
A. INFRARED AVORPTION <197K>
ASSAY
PROCEDURE
Sample solution: Isa 200 mg yeTryptophan mu 125-mL flask.Dissolve mumusanganiswa we3 mL yeformic acid uye 50 mL yeglacial acetic acid.
Kuongorora: Titrate ne 0.1 N perchloric acid VS, inotarisa mugumo we potentiometrically.Ita shumo isina chinhu, uye ita chero gadziriso inodiwa (ona Titrimetry <541>).Imwe mL ye0.1 N perchloric acid yakaenzana ne20.42 mg yeC11H12N2O2.
Maitiro ekugamuchira: 98.5% ~ 101.5% pane yakaomeswa hwaro
KUSIRIRA
Inorganic Impurities
RESIDUEON IGNITION <281>: NMT 0.1%
CHLORIDE NE SULFATE, Chloride <221>: Chikamu che 0.73-g hachiratidzi chloride kupfuura inoenderana ne0.50 mL ye0.020 N hydrochloric acid (0.05%).[Cherechedza-Zvinyoro pisa gadziriro yemuenzaniso kuti iparadze, kana zvichidikanwa.]
CHLORIDE NE SULFATE, Sulfate <221>: Chikamu che 0.33-g hachiratidzi sulfate kupfuura inoenderana ne0.10 mL ye0.020 N sulfuric acid (0.03%).[Cherechedza-Zvinyoro pisa gadziriro yemuenzaniso kuti inyunguduke, kana zvichidikanwa.
IRON <241>: NMT 30ppm
ZVINORERA METALS, Nzira II <231>: NMT 15ppm
Organic Kusachena
PROCEDURE 1
Mhinduro A: Trifluoroacetic acid mumvura (1 mL/L)
Solution B: Trifluoroacetic acid muacetonitrile uye mvura solution (80:20) (1 mL/L trifluoroacetic acid solution)
Mhinduro yakajairwa: 1.0 mg/L imwe neimwe yeUSP Tryptophan Related Compound A RS uye USP Tryptophan Related Compound B RS mumvura
Sample solution: 10.0 mg/mL ye tryptophan mumvura
Sistimu yekukodzera mhinduro: 1.0 mg/L yeUSP Tryptophan Inoenderana Compound B RS mumvura
Nharembozha: Ona tafura yegradient pazasi.

Chromatographic system (Ona Chromatography <621>, Kukodzera Kwehurongwa.)
Modhi: LC
Detector: UV 220 nm
Column: 4.6-mm × 25-cm;5-µm kurongedza L1
Column tembiricha: 30 °
Kuyerera: 1 mL / min
Jekiseni saizi: 20 µL
Kukodzera kwehurongwa
Sample: System kukodzera mhinduro
Kukodzera kunodiwa
Kutsauswa kwechiyero: NMT 5.0%
Analysis
Samples: Yakajairwa mhinduro uye Sample mhinduro
Verengai muzana yekusachena yega yega isina kutaurwa muchikamu cheTryptophan chakatorwa:
Mhedzisiro = (rU/rS) × (CS/CU) × 100
rU = peak nzvimbo yekusachena yega yega isina kutaurwa muSample solution
rS = nzvimbo yepamusoro ye tryptophan inoenderana nekamu B mune iyo Standard mhinduro
CS = kuungana kweUSP Tryptophan Inoenderana Necompound B RS muiyo Standard solution (µg/mL)
CU = kusangana kweTryptophan muSample solution (µg/mL)
Maitiro ekugamuchira
Kusachena kwese 1: NMT 0.01% yehutsvina hwese hunobuda pamberi peiyo tryptophan peak
Kusachena kwese 2: NMT 0.03% yetsvina yese inobuda mushure meiyo tryptophan peak.[Cherechedza-Usabatanidze chikwiriso chetryptophan ine hukama B.]
Tryptophan inoenderana nemubatanidzwa A: Kana iyo yepamusoro ye tryptophan ine hukama musanganiswa A ikaonekwa muSample solution, wobva waedza Maitiro 2: Limit yeTryptophan Related Compound A, pazasi.
KUITA 2: LIMIT OF TRYPTOPHAN RELATED COMPOUND A
Mhinduro A: 18 mM monobasic sodium phosphate, yakasvibiswa uye yakasvibiswa (pH 2.5), uye acetonitrile (9: 1)
Mhinduro B: 10 mM monobasic sodium phosphate, yakasvibiswa uye yakasvibiswa (pH 2.5), uye acetonitrile (1: 1)
Mhinduro C: Acetonitrile mumvura (7:3)
Mushonga wakakwana: 0.1 mg/L yeUSP Tryptophan Related Compound A RS mumvura
Sample solution: 10.0 mg/mL yeTryptophan mumvura
Nharembozha: Ona tafura yegradient pazasi

Chromatographic system
(Ona Chromatography <621>, System Suitability.)
Modhi: LC
Detector: UV 216 nm
Column: 3.9-mm × 15-cm;5-µm kurongedza L1
Column tembiricha: 30 °
Kuyerera: 1 mL / min
Jekiseni saizi: 20 µL
Kukodzera kwehurongwa
Muenzaniso: Standard solution
Kukodzera kunodiwa
Kutsauswa kwechiyero: NMT 5.0%
Analysis
Samples: Yakajairwa mhinduro uye Sample mhinduro
Verenga iyo muzana ye tryptophan inoenderana nekomboni
A muchikamu cheTryptophan chakatorwa:
Mhedzisiro = (rU/rS) × (CS/CU) × 100
rU = nzvimbo yepamusoro ye tryptophan inoenderana nekamu A mune Sample solution
rS = nzvimbo yepamusoro ye tryptophan inoenderana nekamu A mune iyo Standard mhinduro
CS = kuunganidzwa kweUSP Tryptophan Inoenderana Musanganiswa A RS muiyo Standard solution (µg/mL)
CU = kusangana kweTryptophan muSample solution (µg/mL)
Maitiro ekugamuchira: NMT 10 ppm
ZVIYEDZO ZVAKANAKA
OPTICAL ROTATION, Specific Rotation <781S>: -29.4° kusvika -32.8°
Sample solution: 10 mg/mL, mumvura.[Cherechedza-Kupisa zvinyoronyoro kunyungudika, kana zvichidikanwa.]
PH <791>: 5.5~7.0, mune mhinduro (1 mu100)
KURASUKA PAKUOMESA <731>: Pukuta sampuli pa105 ° kwe3 h: inorasikirwa neNMT 0.3% yehuremu hwayo.
ZVIMWE ZVINODIWA
PACKAGE AND STORAGE: Chengetedza mumidziyo yakavharwa zvakanaka
USP REFERENCE MITIRO <11>
USP L-Tryptophan RS
USP Tryptophan Related Compound A RS
3,3′-[Ethylidenebis(1H-indole-1,3-diyl)]bis[2S) -2-aminopropanoic]asidhi.C24H26N4O4 432.49
USP Tryptophan Inoenderana Musanganiswa B RS
2-Acetamido-3- (1H-indol-3-yl) propanoic acid.C13H14N2O3 246.3