Equiupment: GC chiridzwa (Shimadzu GC-2010)
Koramu: DB-17 Agilent 30mX0.53mmX1.0μm
Yekutanga tembiricha yeovheni: 80 ℃
Nguva yekutanga 2.0min
Rate 15 ℃/min
Yekupedzisira tembiricha muovheni: 250 ℃
Nguva yekupedzisira 20min
Mutakuri gasi Nitrogen
Mode Constant kuyerera
Kuyerera 5.0mL/min
Kupatsanura reshiyo 10:1
Injector tembiricha: 250 ℃
Detector tembiricha: 300 ℃
Jekiseni vhoriyamu 1.0μL
Chengetedzo dzinofanirwa kutorwa pamberi pekuongorora:
1. Condition column pa 240℃ kwemaminetsi mashoma makumi matatu.
2. Geza jekiseni uye chenesa jekiseni liner zvakanaka kuti ubvise zvakasvibiswa zvekuongorora kwekare.
3. Geza, omesa uye uzadze diluent mumabhotoro ekugezesa sirinji.
Diluent kugadzirira:
Gadzirira 2% w / v sodium hydroxide solution mumvura.
Kugadzirira kwakajairwa:
Were nezve 100mg ye (R) -3-hydroxyprrolidine hydrochloride standard mubhodhoro, wedzera 1mL ye diluent uye nyungudika.
Kugadzirira bvunzo:
Weresa angangoita 100mg yebvunzo sampuro mubhodhoro, wedzera 1mL ye diluent uye nyungudutsa.Gadzirira mune duplicate.
Maitiro:
Jekiseni isina chinhu (diluent), gadziriro yakajairwa uye kugadzirira bvunzo uchishandisa pamusoro peGC mamiriro.Rega nhongonya nekuda kwekushaikwa.Nguva yekuchengetedza yepamusoro nekuda kwe (R) -3-hydroxyprrolidine inenge 5.0min.
Cherechedza:
Taura mhinduro seavhareji
Nguva yekutumira: Nov-13-2021