Phthalimide Potassium Munyu CAS 1074-82-4 Kuchena > 99.0% (HPLC)

3. Nzira yekuedza
3.1 Kurasikirwa Pakuomesa (W/W)
3.1.1 Zvishandiso uye zviridzwa
Imwe inopfuura zviuru gumi zvemagetsi chiyero, yemagetsi thermostatic drying oveni, 40 * 25 kuyera bhodhoro, dryer.
Reagent: silica gel
3.1.2 Nzira
1. Chenesa 3 40*25 mabhodhoro ekuyera.
2, isa muchoto pa105 ℃ (± 2 ℃) yakaoma kweawa imwe.
3. Bvisa 40 * 25 bhodhoro rekuyera uye uise muomesi kuti utonhorere 30min.
4, Yakanyatso kuyera 40 * 25 yekuyeresa bhodhoro isina huremu W0.
5. Shandisa 40 * 25 bhodhoro rekuyera kuti ureme samuenzaniso yakavhenganiswa yakaenzana ye3.0000g (W1), inokwana 3.
6, Isa muchoto, bvisa chivharo chebhodhoro, chiise padivi pebhodhoro rekuyeresa, kana hafu vhura chivharo chebhodhoro kuti chiomeswe, buritsa, chinofanirwa kunge chiri chivharo chakanaka chekuyeresa bhodhoro, chioma pa105 ℃ (± 2 ℃) 3 maawa.
7. Bvisa 40 * 25 bhodhoro rekuyera uye uiise mumudziyo wekuomesa kuti utonhorere 30min.
8, Kuyera kwakaringana mushure mekuomesa 40 * 25 huremu hwebhodhoro W2.
Kuverengera formula: Kurasikirwa pakuomesa (%) =(W1-W2)/(W1-W0)×100%
3. 4 Kutsunga zvirimo
3.4.1 Zvishandiso nemidziyo
LC-UV100 Liquid Chromatograph (Shanghai Wufeng Scientific Instrument Co., LTD.)
10ml volumetric bhodhoro, Stainless simbi yekona chipunu uye zvimwe zvine hukama
3.3.2 Nzira
Nzira yekuongorora yePhthalimide Potassium Munyu
3.3.3 Liquid chromatography
Chromatographic mamiriro
Chromatographic column: HypersilC6H5 (C8 iripowo) 4.6*250mm 5um
Kuonekwa wavelength: 210nm
Column tembiricha: 30 ℃
Kuyerera kweyero: 1.0ml/min
Sample concentration: 0.05mg/ml (diluent ndiyo mobile phase)
Reagents
Potassium Phosphate Dibasic (analytical pure)
Phthalimide Potassium Munyu (yakajairwa sampuli)
Acetonitrile (analytical pure)
Mvura yakachena
Nharembozha chikamu: acetonitrile: phosphate buffer (PH:3.5) =40:60
Wema 1.63g Potassium Phosphate Dibasic uye nyungudika mu600ml mvura yakachena, gadzirisa kukosha kwePH kusvika 3.5 ine phosphoric acid, dururira mu1000ml beaker ine 400ml acetonitrile, sanganisa zvakanaka, sefa ine 0.45um yakasanganiswa fiber fibre membrane, dissolve application.
3.3.4 Maitiro
1) Kugadzirisa bvunzo mhinduro (0.05mg/ml)
Iyo 2.5mg sampu yakayerwa nemazvo uye yakaiswa mu50ml volumetric bhodhoro.Iyo mobile phase yakawedzerwa uye yakaderedzwa kusvika pachiyero ne ultrasonic dissolution.
2) Kugadzirisa chiyero mhinduro (1.0mg/ml)
Iyo 2.5mg yakajairwa sampu yakayerwa nemazvo uye yakaiswa mu50ml volumetric bhodhoro.Iyo mobile phase yakawedzerwa uye yakaderedzwa kusvika pachiyero ne ultrasonic dissolution.
3) System usability:
(1) Isa musoro wesefa mu 80% methanol pakutanga, vhura vheji yekupedza uye udonhe kwe 3-5min, uye uvhare vheji yekupedza uye sukurudza kwe10-15min.Zvadaro isa musoro wesefa mu 90% yemvura yakachena, svinura valve yekudhonza uye dhonza 3-5min, vhara valve yekudhonza uye sukurudza 10min.
(2) Wobva wachinja chikamu chakagadzirirwa nhare, chiyero chekuyerera i1.0ml/min, mbiru yekumberi iri pasi pe1000Psi, vhura detector, mirira kusvika mbiru yekumberi yagadzikana, vhura komputa yekushandira, uye pinda online.
(3) Mushure mekunge mbiru yekumberi yakatsiga, vhura detector.Kutanga jekiseni 1 tsono ye mobile phase, wobva wabaya 1 tsono yeyakajairwa sampuli, uye pakupedzisira jekiseni sampuli.
(4) Maverengero mhedzisiro: nzvimbo normalization nzira
(5) Mushure mekupedza basa rekuongorora, vhara detector kutanga, uye wosuka musoro wesefa mumvura yakachena ye 10-15min;Panguva imwecheteyo, valve yejekiseni yakashambidzwa nemvura uye methanol.
(6) Pakupedzisira, pfeka 80% methanol, simbura kwe30min, uye uvhare.
4. Mushure mekupedzwa kwekuongorora, zadza pepa rekuongorora uye laboratory test sheet.