Adenine CAS 73-24-5 Assay 98.0%~102.0% (Titration) Warshada Nadiifinta Sare

Adenine wuxuu ka kooban yahay NLT 98.0% iyo NMT 102.0% ee C 5H5N5, oo lagu xisaabiyo sal qallalan.
AQOONSIGA
A. NUUXDA INFRAREED <197K>
ASSAY
HABKA
Tusaale: 200 mg oo Adenine ah
Banaan: 80 mL oo isku dar ah 100 mL oo glacial acetic acid ah iyo 300 mL oo acetic anhydride ah
Nidaamka Titrimetric
Habka: Titration toos ah
Titrant: 0.1 N halkii chloric acid VS sida soo socota: Ku wareeji 300 mg ee potassium biphthalate ilaa 150-mL beaker iyo, walaaqaya, ku milmi 80 mL oo isku dar ah 100 mL oo ah glacial acetic acid iyo 300 mL ee acetic anhydride.Titrate leh halkii kiiloomitir ee chloric acid.Mid kasta oo 20.42mg ah ee potassium biphthalate waxay u dhigantaa 1 ml oo 0.1 N perchloric acid ah.
Ogaanshaha barta dhamaadka: Potentiometric
Falanqaynta: Ku mil muunada 80 mL oo isku dar ah 100 ml oo ah glacial acetic acid iyo 300 mL oo ah anhydride acetic adigoo walaaqaya, oo titrate ku leh Titrant.Xisaabi boqolkiiba Adenine (C5H5N5) ee qaybta la qaaday:
Natiijadu = [(V - B) × N × F × 100]/W
V = Tusaalaha mugga titrant (mL)
B = mugga titrant maran (mL)
N = titrant caadiga ah (mEq/ml)
F = qodob u dhigma, 135.13 mg/mEq
W = miisaanka Tusaalaha (mg)
Shuruudaha aqbalaadda: 98.0%-102.0% ee salka qallalan
XANUUNKA
wasakhda aan noolaha ahayn
DABINTA HAA DHACAY <281>: NMT 0.1%
Biraha culus, Habka II <231>: NMT 10ppm
Waxyeellada dabiiciga ah
HABKA
pH 7.0 phosphate buffer: Ku milmi 4.54 g oo ah fosfateta potassium monobasic biyaha si aad u samayso 500 ml oo xal ah.Ku mil 4.73 g oo ah dibasic sodium phosphate anhydrous biyo ah si aad u samayso 500 ml oo xal ah.Isku qas 38.9 mL oo ah monobasic potassium fosfateta xalka 61.1 mL oo ah xalka sodium phosphate dibasic.Hagaaji, haddii loo baahdo, iyadoo hoos udhaca ku darida xalka sodium phosphate ee dibisiga ah pH ee 7.0.
Xalka saamiga caadiga ah: Ku mil tiro ku habboon USP Adenine RS biyo kulul, qabow, oo ku milo tiro ahaan biyo si aad u hesho xal isagoo la og yahay 0.19 mg/mL.
Xalalka caadiga ah: Pipet 5-mL qaybo ka mid ah xalalka saamiyada caadiga ah oo loo qaybiyo saddex 100-mL musqul mug leh, oo lagu milo 0.10 N hydrochloric acid, 0.010 N sodium hydroxide, iyo pH 7.0 fosfate, siday u kala horreeyaan, mugga.
Tusaalaha xalalka kaydka: Ku mil tiro ku habboon oo Adenine ah biyo kulul, qabow, oo qiyaas ahaan ku milo biyo si aad u hesho xal la og yahay oo ah 0.19 mg/mL.
Tusaalaha xalalka kaydka: Ku mil tiro ku habboon oo Adenine ah biyo kulul, qabow, oo qiyaas ahaan ku milo biyo si aad u hesho xal la og yahay oo ah 0.19 mg/mL.
Xalalka Tusaalaha: Qaybaha Pipet 5-mL ee saamiga saamiga saamiga saddex qaybood oo 100-mL ah, oo lagu milo 0.10 N hydrochloric acid, 0.010 N sodium hydroxide, iyo pH 7.0 fosfate, siday u kala horreeyaan, mugga.
Xaaladaha Spectrometric
(Fiiri Spectropphotometry iyo Iftiin-kala-firdhinta <851>.)
Qaabka: UV-Vis
Mawjadaha dhererka: 220-320 nm
Unug: 1 cm
Banaan: Biyo
Falanqaynta
Tusaalooyinka: xalalka caadiga ah iyo xalalka Tusaalaha
Shuruudaha aqbalaadda: Nuugista kala duwan, oo lagu xisaabiyo saldhigga engegan, dhererka hirarka ugu badan ee nuugista, mid kasta oo ka mid ah xalalka u dhigma kama duwana in ka badan 2.0%.
IMTIXAANO GAAR AH
LOSSON QALABKA <731>: Ku qalaji muunad 110 ° 4 saac: waxay luminaysaa NMT 1.0% miisaankeeda.
NITROGEN CONTENT, Habka II 〈461〉: 50.2%-53.4%, oo lagu xisaabiyay hab qallalan
SHURUUD DHEERAAD AH
Baakadaha iyo Kaydinta: Ku kaydi weelasha si fiican u xidhan.
HEERARKA Tixraaca USP <11>
USP Adenine RS