L-Glutamic Acid α-Benzyl Ester CAS 13030-09-6 (H-Glu-OBzl) Qiimaynta > 98.5% (HPLC) Tayada Sare

1. Daahirnimada (HPLC)
1.1 Qalabka
Waxqabadka sare ee chromatography dareere, Baaraha UV3000.
Isku dheelitirka falanqaynta elegtarooniga ah
1.2 Reagent
Acetonitrile (darajada chromatographic), TFA (darajada chromatographic)
1.3 Xaaladaha Chromatographic
1.3.1 Tiirka: C18 tiirka, 5 μl * 250 * 4.6 mm
1.3.2 Dhererka mowjadda ogaanshaha: 220nm
Heerka socodka: 1.0 ml/min
Mugga duritaanka: 10 μL (tixraac)
Daawada: acetonitrile
Wakhtiga orodka: 20 daqiiqo
1.4 diyaarinta wajiga wareega
Marxaladda wareega A (0.1% Trifluoroacetic acid Water): si sax ah u nuugo 1.0 ml TFA oo lagu qasi jiray 1000 ml oo biyo ah, isku dar iyo degas.
Marxaladda wareega B (0.1% Trifluoroacetic acid Acetonitrile): si sax ah u nuugo 1.0 ml TFA, oo lagu qasi karo 1000 ml oo leh acetonitrile, isku darka iyo degas.
1.5 Barnaamijka Gradient
Waqtiga (daqiiqo) A% B%
0:00 90 10
10.00 10 90
15.00 10 90
15.01 90 10
20.00 90 10
1.6 Diyaarinta xal muunad
Miisaanka 0.1 g oo muunad ah oo ku milan acetonitrile oo ku milo 100ml.Si fiican u rux si aad mustaqbalka u isticmaasho, ama isku-fiirsasho isku mid ah.U diyaari laba muunado oo isbarbar socda.
1.7 Tusaalaha Go'aaminta
U falanqeyso muunada iyadoo la raacayo nidaamka muunada ee soo socota:
1) In ka badan 1 duritaan oo xal maran ah
2) 1 duritaan oo muunad xal ah 1#
3) 1 duritaan oo muunad xal ah 2#
1.8.0 Xisaabinta
1.8.1 Habka caadiga ah ee aagga ugu sarreeya ayaa la isticmaalay si loo xisaabiyo nadiifnimada HPLC.
1.8.2 Macnaha qaraabo leexinta nadiifinta laba irbadood waa in aanu ka badnayn 1%
1.8.3 Haddii natiijooyinka labada irbadood ay buuxiyaan shuruudaha aqbalaadda, qaado celceliska daahirsanaanta natiijada kama dambaysta ah.