Chenodeoxycholic Acid (CDCA) CAS 474-25-9 Qiimaynta ≥98% (Aasaaska Qalalan)

Chenodeoxycholic acid
C24H40O4: 392.57
3a,7a-Dihydroxy-5b-cholan-24-oic acid [474-25-9]
Chenodeoxycholic acid, marka la qalajiyo, waxa ku jira wax aan ka yarayn 98.0z oo aan ka badnayn 101.0% ee C24H40O4.
Sharaxaada Chenodeoxycholic Acid waxay u dhacdaa sida caddaan, kiristaalo, budada crystalline ama budada.
Waxay si xor ah ugu milmi kartaa methanol iyo ethanol (99.5), waxay ku milmi kartaa acetone, oo aan si dhab ah loogu milmin biyaha.
Aqoonsiga Go'aami nooca nuugista infrared ee Chenodeoxycholic Acid, oo hore loo qalajiyey, sida lagu faray habka potassium bromide disk ee hoos yimaada Spectrophotometry Infrared <2.25>, oo barbar dhig spectrum iyo Spectrum tixraaca: labadaba spectra waxay muujinayaan xoojinta nuugista ee tirooyinka hirarka isku midka ah.
Wareegga indhaha <2.49> [a] 20D: +11.0 °~ +13.0° (ka dib qalajinta, 0.4 g, ethanol (99.5), 20 ml, 100 mm).
Barta dhalaalka <2.60> 164~169℃
Nadiifinta (1) Chloride <1.03>-Ku mil 0.36 g of Chenodeoxycholic Acid 30 mL oo methanol ah, ku dar 10 ml oo nitric acid ah iyo biyo si aad u samayso 50 mL, oo ​​tijaabi xalkan.U diyaari xalka xakamaynta sida soo socota: Ilaa 1.0 ml oo ah 0.01 mol/L hydrochloric acid VS ku dar 30 ml oo methanol ah, 10 ml oo ah nitric acid iyo biyo si aad u samayso 50 mL (aan ka badnayn 0.1%).
(2) Biraha culus <1.07>- Ku wad 1.0 g oo Chenodeoxycholic Acid sida waafaqsan Habka 4, oo samee tijaabada.Ku diyaari xalka xakamaynta 2.0 mL ee Xalka Lead Heerka (aan ka badnayn 20 ppm).
(3) Barium-ilaa 2.0 g oo ah Chenodeoxycholic Acid ku dar 100 ml oo biyo ah, isku kari ilaa 2 daqiiqo.Xalkan ku dar 2 mL oo ah hydrochloric acid, karkari 2 daqiiqo, shaandhayso qaboojinta ka dib, oo ku maydh shaandhada biyo ilaa aad ka hesho 100 mL oo ah shaandhaynta.Ilaa 10 mL ee sifaynta ku dar 1 mL oo ah asiidh sulfuric ah oo la walaaqo: wax turbidity ah ma muuqan.
(4) Walxaha laxidhiidha-Mila 0.20 g of Chenodeoxycholic Acid isku dar ah acetone iyo biyo (9:1) si aad u samayso 10 mL oo sax ah, una isticmaal xalkan sida xalka muunada.Si gooni ah, u milmi 10 mg oo lithocholic acid ah oo loogu talagalay chromatography-lakabka khafiifka ah ee isku dhafka acetone iyo biyaha (9: 1) si aad u sameyso 10 ml oo sax ah.Dhuumaha 2 mL ee xalkan, ku dar isku dar ah acetone iyo biyo (9: 1) si aad u sameyso 100 ml oo sax ah, oo u isticmaal xalkan sida xalalka caadiga ah (1).Si gooni ah, u milmaan 10 mg of ursodeoxycholic acid isku dar ah acetone iyo biyo (9: 1) si aad u sameyso 100 ml oo sax ah, oo u isticmaal xalkan sida xalka caadiga ah (2).Si gooni ah, u milmaan 10 mg ee cholic acid chromatography khafiif ah ee isku dhafka acetone iyo biyaha (9: 1) si aad u sameyso 100 ml oo sax ah, oo u isticmaal xalkan sida xalka caadiga ah (3).Dhuumaha 1 mL ee xalka muunada, kuna dar isku dar ah acetone iyo biyo (9:1) si aad u samayso 20 mL.Pipet 0.5 mL, 1 ml, 2 ml, 3 ml iyo 5 mL ee xalkan, ku dar isku dar ah acetone iyo biyo (1) mid kasta oo iyaga ka mid ah si sax ah 50 mL, oo ​​u calaamadee xalalka sida xal caadiga ah A, xal caadiga ah. B, Xalka caadiga ah C, Xalka caadiga ah D iyo xalalka E, siday u kala horreeyaan.Ku samee tijaabada xalalkan sida lagu faray Chromatography-ka khafiifka ah<2.03>Bar 5 mL mid kasta oo ka mid ah xalalka muunada, xalalka caadiga ah (1), (2), (3) iyo xalalka caadiga ah A, B, C, D iyo E ee saxan jel silica ah oo loogu talagalay chromatography khafiif ah.Ku samee saxanka isku dar ah 4-methyl-2-pentanone, toluene iyo formic acid (16:6:1) ilaa fogaan dhan 15 cm, ku qalaji saxanka, oo sii qallaji 1209C ilaa 30 daqiiqo.Isla markiiba, ku buufi si siman xal phosphomolybdic acid n-hydrate ee ethanol (95) (1 in 5) saxanka dushiisa, kuleyl 120 ℃ 2 ilaa 3 daqiiqo: barta u dhiganta goobta leh xalka caadiga ah (1) ma ahan mid ka xoog badan meesha leh xalka caadiga ah (1), barta u dhiganta meesha leh xalka caadiga ah (2) kama badna meesha xalka caadiga ah (2), iyo barta u dhiganta goobta. iyadoo xalka caadiga ah (3) aanu ka xoog badnayn meesha leh xalka caadiga ah
(3).Marka la barbardhigo dhibco leh xalalka caadiga ah ee A, B, C, D iyo E, dhibco kale oo aan ahayn meesha ugu muhiimsan iyo kuwa kale ee aan kor ku soo sheegnay maaha kuwo ka xoog badan barta xalka caadiga ah E, iyo wadarta cadadka kuwaas oo aan ka badnayn 1.5%.
Luminta qallajinta <2.41> In aan ka badnayn 1.5% (1 g, 105 ℃, 3 saacadood).
Hadhaaga dabka <2.44> In aan ka badnayn 0.1% (1 g).
Assay Si sax ah u miisaan qiyaastii 0.5 g of Chenodeoxycholic Acid, oo hore loo qalajiyey, ku milmi 40 ml ethanol (95) iyo 20 ml oo biyo ah, iyo titrate <2.50> oo leh 0.1 mol/L sodium hydroxide VS (titration potentiometric).U samee go'aan madhan si la mid ah, oo samee wixii sixid ah oo lagama maarmaan ah.
ML kasta oo ah 0.1 mol/L sodium hydroxide VS ＝ 39.26 mg oo ah C24H40O4
Weelasha iyo weelasha kaydinta - weelasha adag.