D-Leucine CAS 328-38-1 HD-Leu-OH Assay 98.5~101.5% Warshada Tayada Sare

pH
Qaado 0.50g ee alaabtan, ku dar 50ml oo biyo ah, kuleyl si aad u milmaan, qaboojiso, oo go'aamiso sida waafaqsan sharciga (Guud 0631), qiimaha pH waa 5.5 ~ 6.5.
Gudbinta Xalka
Qaado 0.50g oo badeecadan ah, ku dar 50ml oo biyo ah, kulayl si ay u milmaan, qabow, spectrophotometry ultraviolet-muuqda (Sharciga Guud 0401), cabir gudbinta 430nm dhererka hirarka, aan ka yarayn 98.0%.
Chloride
Qaado 0.25g ee alaabtan oo hubi si waafaqsan sharciga (Xeerka Guud 0801).Marka la barbar dhigo xalka kantaroolka ee ka samaysan 5.0 ml ee xalalka sodium chloride caadiga ah, waa in aanu noqon mid aad u badan (0.02%).
Sulfate
Qaado 1.0g oo alaabtan ah oo hubi si waafaqsan sharciga (xeerka guud 0802).Marka la barbar dhigo xalka kantaroolka ee laga sameeyay 2.0 ml ee xalka potassium sulfate ee caadiga ah, waa in aanu noqon mid aad u badan (0.02%).
Cusbada Ammonium
Qaado badeecadan 0.10g, hubi sida waafaqsan sharciga (Xeerka Guud 0808), oo barbar dhig xalka kantaroolka ee laga sameeyay xalka ammonium chloride ee caadiga ah 2.0 ML, ma qoto dheer (0.02%).
Amino acid kale
Qaado qadar ku habboon alaabtan, ku dar biyo si aad u milmaan oo u milo si aad u samayso xal ka kooban 20mg halkii lml sida xal tijaabo ah;Qaado 1ml cabbirka saxda ah oo ku rid weel cabbir ah 200ml, ku mil miisaanka biyo, rux, sida xal xakamaynta;Qaado qadarka saxda ah ee tixraaca leucine iyo tixraaca valine, oo ku rid isla weelka cabbirka, biyaha ayaa lagu daray in la milo oo la qaso si loo diyaariyo xal ka kooban 0.4mg midkiiba 1ml si nidaam ku habboon.Marka loo eego chromatography lakabka khafiifka ah (General 0502), nuugo saddexda xal ee kor ku xusan mid kasta 5 u1, siday u kala horreeyaan, isla silica gel G saxan lakab khafiif ah, oo leh n-butanol-water-glacial acetic acid (3: 1: 1) horumarinta, ka dib marka la geeyo, qalajinta hawada, ku buufinta ninhydrin ee dareeraha acetone (1-50), kuleylinta 80 ° C ilaa dhibco ka soo baxaan, iyo baaritaanka isla markiiba.Xalka xakamaynta waa inuu muujiyaa meel cad, iyo nidaamka xalka lagu dabaqi karo waa inuu muujiyaa laba dhibcood oo gebi ahaanba kala duwan.Haddii xalka imtixaanku muujiyo dhibco wasakhaysan, midabku waa inaanu ka qoto dheerayn (0.5%) marka loo eego meesha ugu muhiimsan ee xalka xakamaynta.
Khasaare xagga qalajinta
Qaado alaabtan, qallaji at 105 ℃ 3 saacadood, luminta miisaanka waa in aanu dhaafin 0.2% (Sharciga Guud 0831).
Hadhaaga dabka
Qaado 1.0g oo alaabtan ah oo hubi si waafaqsan sharciga (xeerka guud 0841).Inta hadhay waa in aanay ka badnayn 0.1%.
Cusbada Birta
Qaado 1.5g oo alaabtan ah oo hubi si waafaqsan sharciga (Xeerka Guud 0807).Marka la barbardhigo xalka xakamaynta ee laga sameeyay 0.001% ee xalalka birta caadiga ah, ma noqon doonto mid qoto dheer ().
Biraha culus
Hadhaaga ku hadhay shayga qaadashada haraaga dabka waa in aanu ka koobnaan in ka badan 10 qaybood halkii milyan oo bir culus ah marka sharcigu eego (Mabaadi'da Guud 0821, Law II).
Cusbada Arsenic
Ku dar 5ml oo biyo ah, 1ml sulfuric acid iyo 10ml sulfurous acid, kulayl mugga qiyaastii 2ml ee qubeyska biyaha, ku dar 5ml biyo ah, ku dar Dropwise ammonia ilaa tusaha phenolphthalein uu yahay dhexdhexaad, ku dar hydrochloric acid 5ml, ku dar biyo si aad u sameyso 28ml , marka loo eego kormeerka sharciga (Mabaadi'da Guud 0822 Sharciga koowaad), waa in uu u hoggaansamo qodobbada (0.0001%).
Bakteeriyada Endotoxin
Qaado badeecadan, hubi sida waafaqsan sharciga (Guud 1143), lg kasta oo leucine ah oo ay ku jiraan endotoxin waa inuu ka yaraado 25EU.(Dirridda)
328-38-1 - Go'aaminta nuxurka
Qaado alaabtan oo ku saabsan 0.1g, miisaan sax ah, ku dar anhydrous formic acid 1 ml kala diri, ku dar glacial acetic acid 25ml, sida uu qabo habka titration potentiometric (xeerka guud 0701), oo leh xal titration perchloric acid (O. 1 mol/L) titration , natiijadii titration-kana waxaa lagu saxay imtixaan maran.Mid kasta oo 1 ml oo ah xalka titration perchloric acid (0.1 mol/L) waxay u dhigantaa 13.12 mg ee C6H13N02.