D-Tryptophan CAS 153-94-6 (HD-Trp-OH) Assay 98.5 ~ 101.0% Warshada

Aysiidhka
Qaado 1g oo alaabtan ah, ku dar 50ml oo biyo ah si aad u milmaan, oo cabbir sida uu qabo sharciga (Xeerka Guud 5.4).Qiimaha pH waa inuu ahaado 5.4 ~ 6.4.
Gudbinta xalka
Qaado 0.5g oo alaabtan ah, ku dar 2mol/L hydrochloric acid 20ml si ay u milmaan, marka loo eego spectrophotometry-ka muuqda ee ultraviolet (Sharciga Guud 0401), cabir gudbinta 430nm dhererka hirarka, aan ka yarayn 95.0%.
Chloride
Qaado 0.25g ee alaabtan oo hubi si waafaqsan sharciga (Xeerka Guud 0801).Marka la barbar dhigo xalka kantaroolka ee laga sameeyay 0.02% ee xalka sodium chloride caadiga ah, waa in aanu noqon mid xoog badan ().
Sulfate
Qaado l.0g alaabtan oo hubi si waafaqsan sharciga (Xeerka Guud 0802).Marka la barbar dhigo xalka kantaroolka ee laga sameeyay 0.02% ee xalka potassium sulfate ee caadiga ah, waa in aanu noqon mid xoog badan ().
Cusbada Ammonium
Qaado 0.10g ee alaabtan oo hubi si waafaqsan sharciga (Xeerka Guud 0808).Marka la barbar dhigo xalka kantaroolka ee laga sameeyay 0.02% ee xalka ammonium chloride caadiga ah, ma noqon doono mid qoto dheer ().
Amino Acids kale
Qaado 0.30g oo alaabtan ah, ku rid weel cabbiraadda 20ml, ku dar 1 mol/L hydrochloric acid 1 ml iyo biyo si aad u milmaan, biyo ku milo miisaanka, si fiican u rux, xal tijaabo ah;Si sax ah u qaado 1 ml, ku rid weel 200ml ah oo cabbir ah, ku milo si aad biyo u cabbirto, si fiican u rux, una isticmaal sidii xal kontorool ah.Qaado 10mg oo ah kantaroolka tryptophan iyo kontoroolka tyrosine, oo ku rid isla 25ml dhalada cabbiraadda, 1 ml oo ah 1 mol/L hydrochloric acid iyo qadar ku habboon oo biyo ah ayaa lagu daray si ay u milmaan.Xalka waxaa lagu qasi jiray miisaanka biyaha ka dibna la ruxay si loo sameeyo xal ku habboon nidaamka.Marka loo eego chromatography lakabka khafiifka ah (General 0502), nuugo saddexda xal ee kor ku xusan 2 μL kasta, siday u kala horreeyaan, isla silica gel G saxan lakab khafiif ah, oo leh n-butanol-glacial acetic acid-biyaha (3: 1: 1) si aad u faafiso, u fidiso, u engegto, ku buufiso ninhydrin oo ku jira xal acetone (1-50), kuleyl 80 ℃ ilaa barta * ka muuqato, oo isla markiiba arag.Xalka xakamaynta waa inuu muujiyaa meel cad, iyo nidaamka xalka lagu dabaqi karo waa inuu muujiyaa laba dhibcood oo gebi ahaanba kala duwan.Haddii xalka imtixaanku muujiyo dhibco wasakhaysan, midabku waa inaanu ka qoto dheerayn (0.5%) marka loo eego meesha ugu muhiimsan ee xalka xakamaynta.
Khasaare xagga qalajinta
Qaado alaabtan, qallaji at 105 ℃ 3 saacadood, luminta miisaanka waa in aanu dhaafin 0.2% (Sharciga Guud 0831).
Hadhaaga dabka
Qaado 1.0g oo alaabtan ah oo hubi si waafaqsan sharciga (xeerka guud 0841).Inta hadhay waa in aanay ka badnayn 0.1%.
Cusbada Birta
Qaado alaabtan 1.0g gubashada iyo dambaska ka dib, ku dar hydrochloric acid (2ml) haraaga, ku qalaji biyaha qubeyska, ka dibna ku dar basbaaska hydrochloric acid (4ml), ku milmaan kulayl yar, ku dar biyo (30ml) iyo ammonium persulfate (50mg), oo u hubi si waafaqsan sharciga (Xeerka Guud 0807), waa inuusan ka qoto dheerayn (0.002%) marka loo eego xalka kantaroolka ee ka samaysan birta caadiga ah.
Biraha culus
Hadhaaga ku hadhay shayga qaadashada haraaga dabka waa in aanu ka koobnaan in ka badan 10 qaybood halkii milyan oo bir culus ah marka sharcigu eego (Mabaadi'da Guud 0821, Law II).
Cusbada Arsenic
Qaado 2.0g oo badeecadan ah, ku dar 5ml oo ah hydrochloric acid iyo 23ml oo biyo ah si aad u milanto, hubi sida uu qabo sharciga (xeerka guud 0822 sharciga koowaad), waa in uu u hoggaansamaa qodobada (0.0001%).
Bakteeriyada Endotoxin
qaado alaabtan, ku dar bini'aadamka baaritaanka endotoxin, oo kulayl 80 ℃ si aad u milmaan, hubi sida waafaqsan sharciga (General 1143), lg kasta oo tryptophan ah oo ay ku jiraan endotoxin waa in uu ka yaraado 50EU.(Dirridda)
Go'aaminta nuxurka
Qaado alaabtan oo ku saabsan 0.15g, miisaan sax ah, ku dar anhydrous formic acid 3ml kala diri, ku dar glacial acetic acid 50ml, sida ku cad habka titration iman kara (Xeerka Guud 0701), oo leh xal titration hydrochloric acid (0.1 mol / L) titration, iyo Natiijooyinka titration-ka ayaa lagu saxay imtixaan maran.Mid kasta oo 1 ml oo ah xalka titration perchloric acid (0.1 mol/L) waxay u dhigantaa 20.42mg ee C11H12N2O2.