EDC·HCl CAS 25952-53-8 Isku-xidhka Nadiifnimada Reagent>99.0% (T) Warshada

1- (3-Dimethylaminopropyl) -3-Ethylcarbodiimide Hydrochloride (EDC · HCl) (CAS: 25952-53-8) waa budo crystalline cad, si fudud u deliquescent, milmay biyaha, milma ee ethanol, loo isticmaalo sida firfircoonida reagent for carboxyl. kooxaha ku jira synthesis amide, oo sidoo kale loo isticmaalo firfircoonida kooxaha fosfatiyaha, isku-xidhka borotiinnada iyo asiidhyada nucleic-ka, iyo soo-saarka immunocouplers.EDC-HCl waxaa loo isticmaalaa qiyaasta pH ee 4.0-6.0 waxaana badanaa loo isticmaalaa N-Hydroxysuccinimide (NHS) ama N-Hydroxysulfosuccinimide sodium cusbada si loo hagaajiyo waxtarka isku-xirnaanta.
EDC · HCl waa karbodiimide biyo-milmi kara, oo si weyn loogu isticmaalo isku xirka peptide.EDC·HCl waxa loo isticmaalaa sidii wakiil firfircooni karboksiil ah.Waxay door muhiim ah ka ciyaartaa kicinta biomolecules waaweyn ee la xidhiidha N-hydroxysuccinimide.Waxa kale oo loo istcimaalaa acylation of phosphoranes.
Kala soocida biyaha-milmida ee karbodiimide faa'iido u leh isku xirka borotiinnada iyo polypeptides.Loo isticmaalo in lagu beddelo reseptors NMDA iyo sidii wakiilka isku ururinta ee isku-dhafka peptide.Faa'iidada ugu weyn ee isku xirka EDAC waa ka saarida fudud ee reagent-ka xad-dhaafka ah iyo urea u dhiganta iyadoo lagu dhaqo aashito ama biyo.Carbodiimides waxay kicisaa samaynta curaarta amide, asiidhyada karboxilic, iyo amines iyagoo hawlgeliya karboksilate si ay u sameeyaan O-acylurea.Dhexdhexaadkan waxaa si toos ah u weerari kara amiin si uu u sameeyo amide.EDAC waxa loo sii daayaa sida sooshalka urea ee milmi kara.
EDC · HCl waxaa loo isticmaali karaa isku dhafka amides.Waxaa loo isticmaalaa sidii wakiilka isku-xidhka ee isku-dhafka esters-ka ee asiidhyada carboxylic iyadoo la adeegsanayo dimethylaminopyridine sida kicinta.
Inta badan waxaa jira saddex walxood oo si caadi ah loo isticmaalo: DCC, DIC iyo EDC·HCl.Isticmaalka nooc ka mid ah wakiilka kondhomiyaha guud ahaan wuxuu u baahan yahay in lagu daro kicinta acylation ama firfircoonayaasha, sida DMAP, HOBt, iwm. Katalistada looma isticmaalin in lagu beddelo ester-ka firfircoon ee u dhigma ama amide firfircoon, dib ayaa loo habayn doonaa oo u dhigma mid deggan oo ka soo baxay HOL (Jidka B).Firfircoonida uumiga: Waxaa jira noocyada soo socda ee firfircoonida uumiga.Waqtigan xaadirka ah, 4-N, N-dimethylpyridine (DMAP) ayaa si ballaaran loo isticmaalay si loo kiciyo falcelinta acylation kala duwan.Mararka qaarkood 4-PPY waa la isticmaali karaa marka saameynta catalytic ee DMAP aysan fiicneyn.Marka loo eego suugaanta khuseysa, awooddeeda kicinta waxay kun jeer ka sarraysaa DMAP.ka mid ah saddexda koob ee sida badan loo isticmaalo, DCC iyo DIC waa ka jaban yihiin.Guud ahaan DCC iyo DMAP ayaa la wada isticmaalaa.Mid ka mid ah faa'iido darrada ugu weyn ee isticmaalka DCC waa in sheyga kale ee falcelinta, dicyclohexylurea, uu leeyahay milan yar ee wajiga guud ee organic laakiin waa wax yar oo milmay, sidaas darteed way adagtahay in si fiican looga saaro iyada oo loo marayo hababka nadiifinta ee sida caadiga ah loo isticmaalo, recrystallization, chromatography tiirka, iwm.;sababtoo ah milanka dicyclohexylurea ee ether waa mid aad u yar marka loo eego dareerayaasha kale, sidaas darteed, daaweynta noocan ah ee falcelinta guud ahaan waxay soo saartaa dareeraha falcelinta waxayna ku daraysaa ether, waxayna shaandheysaa inta badan dicyclohexylurea ka hor daaweyn dheeraad ah.Sababtoo ah diisopropylurea ay soo saartay DIC ayaa si fiican u milmay guud ahaan dareerayaasha organic, guud ahaan waxaa loo isticmaalaa isku-darka kiimikada isku-dhafka ah ee wejiga adag.EDCI hadda waxaa inta badan loo isticmaalaa kimistariga daawada.Mid ka mid ah sifooyinkeeda ugu muhiimsan ayaa ah in kaadida ay dhaliso falcelinta ka dib ay tahay mid biyo-milmi leh oo ay fududahay in la dhaqo.Guud ahaan, EDCI waxay la socotaa HOBt (xusuusnow: falcelintan HOBt guud ahaan waa lama huraan, haddii kale waxay keeni kartaa dhalidda uumiga oo aad u hooseeya).Mararka qaarkood haddii xannibaadda-mawqifka aashitada ay weyn tahay ama ay jirto koox ka-noqosho elektaroonig ah, fal-celintu waxay ku sii jiri doontaa tallaabada ester-ka firfircoon (ester-ka firfircoon wuxuu leeyahay calaamad xoog badan oo mass spectrum ah waxaana lagu ogaan karaa MS ama LC-MS).