Fluorescamine CAS 38183-12-9 daahirnimo>98.0% (HPLC)

Fluorescamine (CAS: 38183-12-9) waa reagent aan fluorescent ahayn oo si sahal ah uga falceliya xaalado khafiif ah oo leh amines aasaasiga ah ee amino acids iyo peptides si ay u sameeyaan xeryahooda xasiloon, aad u sarreeya.Falcelinta waxay ku dhacdaa xaalado khafiif ah.Asalkii hore oo hooseeya sababtoo ah hydrolysis.falanqeeye falanqeeye.Si loo ogaado fluorescent ee acids amino, peptides iyo borotiinada.Fluorescamine waxay door muhiim ah ka ciyaartaa sidii reagent-ka ogaanshaha amines, peptides iyo borotiinada.Waxa kale oo loo istcimaalaa sidii substrate si loo cabbiro dhaqdhaqaaqa borotiinka.Waxaa loo adeegsaday ogaanshaha iyo tirada haraaga aminopenicillins iyo sulfonamides ee malabka ee HPLC.Waxay ka heleysaa codsi go'aaminta lisinopril ee plasma iyo kaadida aadanaha.Wuxuu si ku filan u xannibaa termini-ka amino ee dhawaanta la soo saaray ee falanqaynta isku xigxiga borotiinka.Fluorescamine waa amine-fluorogenic reagent caan ah oo loogu talagalay go'aaminta uruurinta borotiinka ee xalalka iyo gelsyada.Waxa kale oo loo istcimaalaa in lagu falanqeeyo aminyada miisaanka kellika hoose ee TLC, HPLC iyo electrophoresis capillary.Fluorescamine waa nonfluorescent laakiin waxay si fudud ula falgashaa amines aliphatic aasaasiga ah, oo ay ku jiraan kuwa peptides iyo borotiinada, si ay u soo saaraan qulqulo buluug-cagaaran ah oo xoog leh oo ku farxi kara iftiinka UV.Dufanka amine wuxuu leeyahay ugu badnaan nuugista 385 nm iyo ugu badnaan fluorescence 486 nm.