Glycine CAS 56-40-6 (H-Gly-OH) Qiimaynta 98.5 ~ 101.5% Warshada Tayada Sare

USP35-NF30
Qeexitaan
Glycine wuxuu ka kooban yahay NLT 98.5% iyo NMT 101.5% glycine (C2H5NO2), oo lagu xisaabiyo sal qallalan.
AQOONSIGA
A. NUUXDA INFRAREED <197M>
ASSAY
HABKA
Tusaalaha: 150 mg oo Glycine ah
Banaan: 100 ml oo ah glacial acetic acid
Nidaamka Titrimetric
(Fiiri Titrimetry <541>.)
Habka: Titration toos ah
Titrant: 0.1 N halkii kiiloric acid VS
Ogaanshaha barta dhamaadka: Muuqaal
Falanqaynta: Ku mil muunadda 100 mL oo ah glacial acetic acid, oo ku dar 1 dhibcood oo violet crystal TS.Titrant ku dheji Titrant ilaa barta dhamaadka cagaaran.Samee go'aanka maran.
Xisaabi boqolkiiba glycine (C2H5NO2) ee muunadda la qaaday:
Natiijadu = {[(VS − VB) × N × F]/W} × 100
VS = Mugga titrant ee uu isticmaalo Tusaalaha (mL)
VB = Mugga titrant ee uu isticmaalo Bannaanka (mL)
N = Caadiga dhabta ah ee Titrant (mEq/ml)
F = qodob u dhigma, 75.07 mg/mEq
W = Tusaalaha miisaanka (mg)
Shuruudaha aqbalaadda: 98.5% -101.5% ee salka qallalan
XANUUNKA
• Hadhaaga dabka <281>: NMT 0.1%
• Kloride iyo sulfate, Chloride <221>
Xalka caadiga ah: 0.10 ml oo ah 0.020 N hydrochloric acid
Tusaalaha: 1 g oo ah Glycine
Shuruudaha aqbalaadda: NMT 0.007%
• Kloride iyo Sulfate, Sulfate <221>
Xalka caadiga ah: 0.20 ml oo ah 0.020 N sulfuric acid
Tusaalaha: 3 g oo ah Glycine
Shuruudaha aqbalaadda: NMT 0.0065%
• Biraha culus, Habka I <231>: NMT 20 ppm
• Biraha culus, Habka I <231>: NMT 20 ppm
Tusaalaha xalalka: 100 mg/mL oo Glycine ah
Falanqaynta: Karkari 10 ml oo ah xalka Tusaalaha 1 daqiiqo, oo meel dhig 2 saacadood.
Shuruudaha aqbalaadda: Xalku wuxuu u muuqdaa mid cad oo guuraya sida 10 ml oo isku mid ah oo aan la karinin.
IMTIXAANO GAAR AH
• luminta qallajinta <731>: Ku qalaji muunad 105° 2 saac: waxay luminaysaa NMT 0.2% miisaankeeda.
SHURUUD DHEERAAD AH
Baakadaha iyo Kaydinta: Ku kaydi weelasha si fiican u xidhan.
• HEERARKA Tixraaca USP <11>
USP Glycine RS

Pharmacopoeia Jabbaan JP17
Glycine, marka la qalajiyo, waxay ka kooban tahay wax aan ka yarayn 98.5% ee Glycine (C2H5NO2).
Sharaxaada Glycine waxay u dhacdaa sida cad, crystals orcrystalline budada.Waxay leedahay dhadhan macaan.Waxay si xor ah ugu milmay biyaha iyo aashitada formic, iyo ficil ahaan aan lagu milmi karin ethanol (95).Waxay muujinaysaa polymorphism crystal.
Aqoonsiga Go'aami nooca nuugista infrared ee Glycine, oo hore loo qalajiyey, sida lagu faray habka diskka potassiumbromide ee hoos yimaada Infrared Spectrophotometry <2.25>, oo barbar dhig spectrum iyo Spec-trumka Tixraaca: labadaba spectra waxay muujinayaan xoojinta nuugista isku midka ah ee tirooyinka hirarka isku midka ah.Haddii wax farqi ahi u dhexeeyo spectra, ku milmi Glycine biyaha, uumi-baxa biyaha, oo ku celi tijaabada hadhaaga.
pH <2.54> Ku mil 1.0 g oo Glycine ah 20 ml oo biyo ah: pH ee xalku waa inta u dhaxaysa 5.6 iyo 6.6.
daahirnimo
(1) Cadnaanta iyo midabka xalka-Ku mil 1.0 gof Glycine 10 ml oo biyo ah: xalku waa cad yahay oo midab-la'aan.
(2) Chloride <1.03>-Ku samee tijaabada 0.5 g oo Glycine ah.Ku diyaari xalka xakamaynta 0.30 ml oo ah 0.01mol/L hydrochloric acid VS (aan ka badnayn 0.021%)
(3) Sulfate <1.14>-Ku samee tijaabada 0.6 g oo Glycine ah.Ku diyaari xalka xakamaynta 0.35 mL oo ah 0.005mol/L sulfuric acid VS (aan ka badnayn 0.028z).
(4) Ammonium <1.02>- Samee tijaabada adigoo isticmaalaya 0.25 g oo Glycine ah.Ku diyaari xalinta xakamaynta 5.0 mL ee Heerka Ammonium Solution (aan ka badnayn 0.02%).
(5) Biraha culus <1.07>- Ku sii wad 1.0 g oo Glycine ah sida waafaqsan Habka 1, oo samee tijaabada.Ku diyaari xalka xakamaynta 2.0 mL ee Xalka Lead Heerka (aan ka badnayn 20 ppm).
(6) Arsenic <1.11>-Ku diyaari xalka tijaabada 1.0 gof Glycine sida ku cad Habka 1, oo samee tijaabada (aan ka badnayn 2 ppm).
(7) Walxaha laxidhiidha-Ku mil 0.10 g oo Glycine ah 25mL oo biyo ah una isticmaal xalkan sida xalka muunada.Dhuumaha 1 mL ee xalka muunada, ku dar biyo si aad u samayso 50 ml oo sax ah.Pipet 5 mL ee xalkan, ku dar biyo si aad u sameyso 20 mL oo sax ah, oo u isticmaal xalkan sida xalalka caadiga ah. Samee tijaabada xalalkan sida lagu hagayo Chromatography khafiif ah<2.03>.Bar 5mL mid kasta oo ka mid ah muunadda muunada iyo xalalka caadiga ah saxan silica gel forthin-lakabka chromatography.Ku samee saxanka isku dar ah 1-butanol, biyo iyo acetic acid (100) (3:1:1) ilaa fogaan dhan 10 cm, oo ku qalaji saxanka 80℃ 30 daqiiqo.Ku buufi si siman xal ninhydrin ah acetone (1kii 50), kuleyl 80 ℃ ilaa 5 daqiiqo: dhibco kale oo aan ahayn meesha ugu muhiimsan ee xalka muunada ma aha kuwo ka xoog badan meesha laga helo xalka caadiga ah.
Khasaaraha qallajinta <2.41> In aan ka badnayn 0.30% (1 g, 105 ℃, 3 saacadood).
Hadhaaga dabka <2.44> In aan ka badnayn 0.10% (1g).
Assay Miisaanka saxda ah ee ku saabsan 80 mg ee Glycine, oo hore loo qalajiyey, ku milmi 3 mL ee formic acid, ku dar 50 ml acetic acid (100), iyo titrate <2.50> oo leh 0.1 mol/L perchloric acid VS (titration potentiometric).Samee go'aan madhan, oo samee wixii sixid ah oo lagama maarmaan ah.
ml kasta oo ah 0.1 mol/L perchloric acid VS ＝7.507 mg of C2H5NO2
Weelasha iyo weelasha kaydinta - weelasha si fiican u xidhan.