L-Cysteine ​​CAS 52-90-4 (H-Cys-OH) Assay 98.5~101.0% (Titration) Warshada Tayada Sare

L-Cysteine ​​​​waxaa ku jira wax aan ka yarayn 98.5% oo aan ka badnayn 101.0% L-Cysteine ​​​​(C3H7NO2S), oo lagu xisaabiyo sal engegan.
Sharaxaada L-Cysteine ​​waxay u dhacdaa sida kiristaalo cad ama budo crystalline cad.Waxay leedahay ur lagu garto iyo dhadhan qafiif ah.Waxay si xor ah ugu milmi kartaa biyaha, iyo ficil ahaan aan lagu milmi karin ethanol (99.5).Waxay ku milmaysaa 1 mol/L hydrochloric acid TS.
Aqoonsiga Go'aami nooca nuugista infrared ee L-Cysteine ​​sida lagu faray habka potassium bromide disk ee hoos yimaada Infrared Spectrophotometry <2.25>, oo barbar dhig spectrum iyo Spectrum tixraaca: labadaba spectra waxay muujinayaan xoojinta nuugista isku midka ah ee tirooyinka hirarka isku midka ah.
Wareegga indhaha <2.49> [a] 20D:+8.0 ~+10.0°
pH <2.54> pH ee xalka loo diyaariyey iyadoo lagu milmay 1.25 g oo L-Cysteine ​​ah 50 ml oo biyo ah waa 4.7 ilaa 5.7.
Nadiif (1) Caddayn iyo midabka xalka-Ku mil 1.0 g oo L-Cysteine ​​ah 20 ml oo biyo ah: xalku waa cad yahay oo aan midab lahayn.
2 biyo si ay u sameeyaan 50 ml.Samee tijaabada adoo isticmaalaya xalkan sidii xalka tijaabada.Ku diyaari xalka xakamaynta 0.35 mL ee 0.01 mol/L hydrochloric acid VS (aan ka badnayn 0.041%)
(3) Sulfate <1.14>-Ku mil 0.6 g oo L-Cysteine ​​ah 30 ml oo biyo ah iyo 3 mL oo ah hydrochloric acid la badhxo, oo ku dar biyo si aad u samayso 50 ml.Samee tijaabada adoo isticmaalaya xalkan sidii xalka tijaabada.U diyaari xalka xakamaynta sida soo socota: Ilaa 0.35 mL oo ah 0.005 mol/L sulfuric acid VS ku dar 3 mL oo ah aashitada hydrochloric-ka iyo biyo si aad u samayso 50 mL.U diyaari xalka tijaabada iyo xalka xakamaynta 4 mL ee barium chloride TS, siday u kala horreeyaan (aan ka badnayn 0.028%).
(4) Ammonium <1.02> -Ku samee tijaabada 0.25 g ee L-Cysteine ​​​​, adoo isticmaalaya distillation hoos cadaadiska hoos u dhaca.Ku diyaari xalinta xakamaynta 5.0 mL ee Heerka Ammonium Solution (aan ka badnayn 0.02%).
(5) Biraha culus <1.07> - Ku sii wad 1.0g ee L-Cysteine ​​​​sida waafaqsan Habka 4, oo samee tijaabada. Diyaari xalka xakamaynta 1.0 mL ee Xalinta Lead Heerka (aan ka badnayn 10 ppm).
(6) Birta <1.10>-U diyaari xalka tijaabada 1.0 g ee L-Cysteine ​​sida ku cad Habka 1, oo samee tijaabada sida ku cad Habka A. Diyaari xalka xakamaynta 1.0mL ee Xalka Birta caadiga ah (aan ka badnayn 10 ppm ).
(7) Walxaha La Xiriira-Ku milmaan 0.10 g L-Cysteine ​​ah oo ku jira xalka N-ethylmaleimide (1 50kiiba) si aad u sameyso 10 ml oo sax ah, u daa 30 daqiiqo, oo u isticmaal xalkan sida xalka muunada.Beet 1 ml oo muunada muunada ah, ku dar biyo si aad u samayso 10 ml oo sax ah, pipet 1 ml ee xalkan, ku dar biyo si aad u samayso 50 ml oo sax ah, una isticmaal xalkan sida xalka caadiga ah (1).Si gooni ah, ugu mil 0.10 g oo L-cystine ah 0.5mol/L hydrochloric acid TS si aad u samayso 20 ml oo sax ah.Dhuumaha 1mL ee xalkan, ku dar biyo si aad u sameyso 100 ml, oo u isticmaal xalkan sida xalalka caadiga ah (2).Ku samee tijaabada xalalkan sida lagu faray Chromatography-ka khafiifka ah<2.03>.Bar 10mL mid kasta oo ka mid ah xalalka muunada iyo xalalka caadiga ah (1) iyo (2) saxan jel silica ah oo loogu talagalay chromatography lakab khafiif ah.Ku samee saxanka isku dar ah 1-butanol, biyo, iyo acetic acid (100) (3: 1: 1) ilaa fogaan ah qiyaastii 10 cm, oo qalaji saxanka 30 daqiiqo 809C. Ku buufin saxanka si siman xal of ninhydrin isku dar ah methanol iyo acetic acid (100) (97:3) (1 in 100), ka dibna kuleyl at 80 ℃ 10 daqiiqo: barta laga helay xal muunad u dhiganta meeshii laga helay xal heerka caadiga ah (2) ma aha mid ka xoog badan barta xalka caadiga ah (2).Sidoo kale, dhibcooyinka aan ahayn meesha ugu muhiimsan iyo dhibcaha kor lagu soo sheegay ee xalka muunada kama badna goobta xalka caadiga ah (1).
Luminta qallajinta <2.41> In aan ka badnayn 0.50% (1 g, vacuum, fosfooraska (V) oxide, 3 saacadood).
Hadhaaga dabka <2.44> In aan ka badnayn 0.1%(1 g).
Assay Si sax ah u miisaam qiyaastii 0.2 g oo L-Cysteine ​​ah, ku rid weel taagan, oo ku mil 20 ml oo biyo ah.Ku mil 4 g of potassium iodide xalkan, isla markiiba ku rid baraf biyo qabow, ku dar 5 mL oo ah hydrochloric acid dilute iyo 25 ml oo ah 0.05 mol/L iodine VS, ku dhaaf meel mugdi ah 20 daqiiqo, ka dibna titrate <2.50 > xaddi xad dhaaf ah oo iodine ah oo leh 0.1 mol/L sodium thiosulfate VS (tusaale: istaarij TS).Samee go'aan maran adigoo isticmaalaya hab la mid ah.
ml kasta oo ah 0.05 mol/L iodine VS ＝12.12 mg of C3H7NO2S
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