L-Cystin CAS 56-89-3 (H-Cys-OH)2 Assay 98.5~101.0% (Titration) Warshada Tayada Sare

Qeexitaan
Cystine waxa uu ka kooban yahay NLT 98.5% iyo NMT 101.5% ee C6H12N2O4S2, sida L-Cystine, oo lagu xisaabiyey hab qalalan.
AQOONSIGA
A. NUUXDA INFRAREED <197K>
B. Wareegga indhaha, Wareegga Gaarka ah <781S>: -215 ilaa -225, oo lagu go'aamiyay 20°
Xalka muunada: 20 mg/mL, in 1 N hydrochloric acid
C. Qiimaha RF ee meesha ugu muhiimsan ee xalka Tusaalaha ee tijaabada ee wasakhaynta Dabiiciga ah waxay u dhigantaa kan xalalka caadiga ah.
ASSAY
HABKA
Xalka muunada: Ku wareeji qiyaastii 0.1 g oo Cystin ah ilaa weel quraarad joogsan, oo ku mil isku dar ah 2 ml oo ah sodium hydroxide (1 ee 20) iyo 10 ml oo biyo ah.Ku dar 10 mL oo ah xalinta potassium bromide (200 g/L oo biyo ah), 50.0 mL oo ah 0.1 N potassium bromate VS, iyo 15 mL oo ah hydrochloric acid (17 in 100).
Islamarkiiba geli joojiyaha galaaska, oo ku qabooji qubeyska biyaha barafka ah.Oggolow inuu istaago isagoo iftiinka ka ilaalinaya 10 daqiiqo.
Nidaamka Titrimetric
(Fiiri Titrimetry <541>)
Qaabka: Titration hadhaaga
Titrant: 0.1 N potassium bromate VS
Dib-u-titrant: 0.1 N sodium thiosulfate VS
Ogaanshaha barta dhamaadka: Colorimetric
U dhigma: ml kasta oo ah 0.1 N potassium bromate VS waxay u dhigantaa 2.403 mg ee C6H12N2O4S2 oo ku salaysan qallalan.
Falanqaynta: Ku dar 1.5 g oo ah potassium iodide, iyo 1min ka dib, titrate leh 0.1 N sodium thiosulfate VS, adoo isticmaalaya istaarijka TS sida tilmaame.Samee go'aan maran, oo samee sixid kasta oo lagama maarmaan ah.
Shuruudaha aqbalaadda: 98.5% ~ 101.5% ee salka qallalan
XANUUNKA
wasakhda aan noolaha ahayn
Hadhaaga dabka <281>: NMT 0.1%
Kloride iyo sulfate, Chloride <221>: NMT 200 ppm.Qayb 0.7-g ah ma muujiso koloriin ka badan oo u dhiganta 0.40 mL oo ah 0.01 N hydrochloric acid.
Kloride iyo sulfate, Sulfate <221> NMT 200 ppm.Qaybta 1.2-g waxay muujinaysaa wax ka badan sulfate oo u dhiganta 0.25 mL oo ah 0.020 N sulfuric acid.
IRON <241>: NMT 10ppm
Biraha culus, Habka I <231>: NMT 10ppm
Waxyeellada dabiiciga ah
HABKA
Xalka caadiga ah: Ku mil tiro USP Cystin RS ee 1 N hydrochloric acid, oo ku milo biyo si aad u hesho xal iyada oo la og yahay fiirsashada 0.02 mg/mL.
Xalka Tusaalaha: Ku milmaan tiro Cystin ah 1 N hydrochloric acid, oo ku milan biyo si aad u hesho xal isagoo leh xaddi la og yahay oo ku saabsan 10 mg/mL.
Xalka ku habboonaanta nidaamka: Kala dir tirada USP Cystin RS iyo USP Arginine Hydrochloride RS ee 1 N hydrochloric acid, oo ku milo biyo si aad u hesho xal iyada oo la og yahay uruurinta qiyaastii 0.4 mg/mL midkiiba.
Nadaamka Chromatographic (Eeg Chromatography <621>, Chromatography ee khafiifka ah.)
Habka: TLC
Adsorbent: 0.25-mm lakabka silica jel chromatographic ah
Codsiga mugga: 5μL
Horumarinta habka dareeraha: Isku dhafka ammonia iyo 2-propanol (3:7)
Daawada reagent: Ku mil 0.2 g oo ninhydrin ah 100 mL oo isku dhafka ah ee butanol iyo 2 N acetic acid (95:5).
Falanqaynta
Tusaalooyinka: Xalka caadiga ah, Xalka Tusaalaha, iyo xalka ku habboonaanta nidaamka
U wad sida lagu faray Chromatography <621>, Chromatography-Lakaarka khafiifka ah.Ka dib markaad hawo-qaboojiso saxanka, ku buufi reagent buufin, oo kulayl inta u dhaxaysa 100 ° iyo 105 ° 15 min.Baar saxanka.chromatogram-ka laga soo qaatay xalka ku habboonaanta nidaamka ayaa muujinaya laba barood oo si cad u kala soocan.
Shuruudaha aqbalaadda: Meel kasta oo labaad oo ka timaadda xalka Tusaalaha kama weyna ama kama xoog badna meesha ugu muhiimsan ee xalinta caadiga ah.
Nijaasooyinka shaqsiga ah: NMT 0.2%
Wadarta wasakhda: NMT 2.0%
IMTIXAANO GAAR AH
QASAARAN QALABKA <731>: Ku qalaji muunad 105 ℃ 3 saac: waxay luminaysaa NMT 0.2% miisaankeeda.
SHURUUD DHEERAAD AH
BAAKAYNTA IYO KAYDINTA: Ku kaydi weelasha si fiican u xidhan, oo ku kaydi heerkulka qolka oo la xakameeyey.
HEERARKA Tixraaca USP <11>
USP Arginine Hydrochloride RS
USP Cystin RS