Levodopa (L-DOPA) CAS 59-92-7 99.0 ~ 100.5% USP BP EP Heerka Ka-hortagga Cudurka Parkinson

Wareegga indhaha [EP]
Kala dir tiro u dhiganta 0.200g ee walaxda qallalan iyo 5g ee hexamethylenetetramine R 10ml oo ah 1mol/L hydrochloric acid oo ku mil 25.oml isla asiidh.U ogolow in xalku istaago isagoo ka ilaalinaya iftiinka ilaa 3 saacadood.Xagalka wareegga indhaha waa -1.27° ilaa -1.34°
Muuqashada Xalka
Ku mil 1.0g ee 1mol/L hydrochloric acid oo ku mil 25ml isla solvnet.Xalku maaha mid ka midab badan marka loo eego xalka tixraaca BY6.
Walxaha La Xiriira [EP]
Ku baadh chromatography lakab khafiif ah, adoo isticmaalaya cellulose chromatography R sida walaxda daahan.
Tijaabada xal-ku milmi 0.1g ee walaxda lagu baaro 5ml of anhydrous formic acid R oo ku mil 10ml methanol R. Isku diyaari isla markiiba ka hor isticmaalka.
Xalka tixraaca (a)-Ku qas 0.5ml ee xalka tijaabada ilaa 100ml methanol R.
Xalka tixraaca (b) -Ku mil 30mg ee tyrosine R ee 1ml of anhydrous formic acid R oo ku qas 100ml methanol R. Isku qas 1ml ee xalkan iyo 1ml ee xalka tijaabada.
Si gaar ah ugu codso saxanka sida guntooyin dhererkiisu yahay 20mm, 1oμl ee xalka tijaabada, 10μl ee xalka tixraaca (a) iyo 20μl ee xalka tixraaca (b).Ku qallaji hawo hadda socota.Samee waddo od 15cm ah adigoo isticmaalaya isku dhafka 50 muga ee butanol R, 25 mug oo glacial acetic acid R iyo 25 mug biyo ah.Ku qalaji saxanka hawo diiran oo hadda jirta kuna buufi isku dar cusub oo la diyaariyey oo mug le'eg oo ah 10 boqolkiiba m/v xal ferric chloride R iyo 5 boqolkiiba m/v xal postassium ferricyanide R. Isla markiiba baar chromatoframs.Bar kasta oo chromatogram-ka ah ee lagu helo xalka tijaabada, marka laga reebo meesha ugu muhiimsan, kama sii xoog badna meesha ku jirta chromatogram-ka ee leh xalka tixraaca (a) ayaa muujinaya.Tijaabadu ma ansaxayso ilaa chromatogram-ka lagu helay xalka tixraaca (b) uu muujiyo, meel ka sarraysa meesha ugu muhiimsan, bar ka duwan oo ka xoog badan barta ku jirta chromatogram-ka lagu helay xal tixraaceed (a).
UV Spectrum [EP]
Ku mil 30.0mg 0.1mol/L hydrochloric acid oo ku mil 100.oml isla asiidh la mid ah.Ku qas 10.0ml ee xalkan ilaa 100.0ml 0.1mol/L hydrochloric acid.La baadhay inta u dhaxaysa 230nm iyo 350nm, xalku wuxuu muujinayaa hal nuug oo ugu badnaan 280nm.Nuugista gaarka ah ee ugu badnaan waa 137 ilaa 147, oo lagu xisaabiyo tixraaca walaxda la qalajiyey.
Khasaare xagga qalajinta
Qaado alaabtan, engegan ilaa miisaan joogto ah 105 ° C, miisaan lumis waa inuusan dhaafin 1.0% (Xeerka Guud 0831).
Hadhaaga dabka (Sulfated)
Qaado l.0g ee Levodopa oo u hubi si waafaqsan sharciga (xeerka guud 0841).Inta hadhay waa in aanay ka badnayn 0.1%.
Biraha culus
Hadhaaga ku hadhay shayga qaadashada haraaga dabka waa in aanu ka koobnaan in ka badan 10 qaybood halkii milyan oo bir culus ah marka sharcigu eego (Mabaadi'da Guud 0821, Law II).
Tijaabada pH
0.10g oo 10ml ah oo H2O ah oo gariiraya 15 daqiiqo.
Go'aaminta nuxurka
Qaado badeecadan oo ku saabsan 0.lg, miisaan sax ah, ku dar anhydrous formic acid 2ml si ay u milaan, ku dar glacial acetic acid 20ml, rux, ku dar tilmaame violet crystal 2 dhibcood, oo leh xal titration perchloric acid (0.1 mol / L) titration si xalku waa cagaar, iyo natiijada titration lagu saxo imtixaan maran.Mid kasta oo 1 ml oo ah xalalka titration perchloric acid (0.1 mol/L) waxay u dhigantaa 19.72mg ee C9H11N04.

Habka Tijaabada JP17

Levodopa, marka la qalajiyo, waxay ka kooban tahay wax aan ka yarayn 98.5% ee Levodopa (C9H11NO4).
Sharaxaada Levodopa waxay u dhacdaa sida caddaan ama wax yar oo cawl ah, crystals ama budada crystalline.Waa bilaa ur.Waxay si xor ah ugu milmaysaa aashitada formic, wax yar ayay ku milmi kartaa biyaha, iyo ficil ahaan aan lagu milmi karin ethanol (95).Waxay ku milantaa hydrochloric acid.pH ee xal buuxa ee Levodopa waa inta u dhaxaysa 5.0 iyo 6.5.Meesha dhalaalka: qiyaastii 275 ℃ (oo leh kala-bax).
Aqoonsiga
(1) Ilaa 5 mL oo xal ah Levodopa (1 in 1000) ku dar 1mL oo ninhydrin TS ah, iyo kulayl 3 daqiiqo gudaha qubeyska biyaha: midab guduudan ayaa soo baxa.
(2) Ilaa 2 mL oo xal ah Levodopa (1kii 5000) ku dar 10 ml 4-aminoantipyrine TS, oo rux: midab cas ayaa soo baxaya.
(3) Ku mil 3 mg ee Levodopa 0.001 mol/L hydrochloric acid TS si aad u samayso 100 mL.Go'aanso nuugista spectrum ee xalka sida lagu hago hoosta Ultravioletvisible Spectrophotometry <2.24>, oo barbar dhig spectrum iyo Spectrum tixraaca: labada spectra waxay muujinayaan xoojinta nuugista isku midka ah ee hirarka dhererka isku midka ah.
nuugista <2.24> E 1%1cm (280 nm): 136-146 (ka dib qalajinta, 30 mg, 0.001 mol/L hydrochloric acid TS, 1000 ml).
Wareegga indhaha <2.49> [a] 20D: -11.5° ~-13.0°(ka dib qalajinta, 2.5 g, 1 mol/L hydrochloric acid TS, 50 ml, 100 mm).
daahirnimo
(1) Caddayn iyo midabka xalka-Ku milmi 1.0 g oo Levodopa ah 20 mL oo ah 1 mol/L hydrochloric acid TS: xalku waa cad yahay oo aan midab lahayn.
2Samee tijaabada adoo isticmaalaya xalkan sidii xalka tijaabada.Ku diyaari xalka xakamaynta 0.30 mL ee 0.01 mol/L hydrochloricacid VS (aan ka badnayn 0.021%).
(3) Sulfate <1.14>-Ku mil 0.40 g oo Levodopa ah 1 ml oo ah hydrochloric acid dilute ah iyo 30 ml oo biyo ah, ku dar biyo si aad u samayso 50 mL.Samee tijaabada adoo isticmaalaya xalkan sidii xalka tijaabada.Ku diyaari xalka xakamaynta 0.25 mL ee 0.005 mol/L sulfuric acid VS (aan ka badnayn 0.030%).
(4) Biraha culus <1.07>- Ku sii wad 1.0 g oo Levodopa ah sida uu qabo Habka 2, oo samee tijaabada.Ku diyaari xalka xakamaynta 2.0 mL ee Xalka Lead Heerka (aan ka badnayn 20 ppm).
(5) Arsenic <1.11>-Ku mil 1.0 g oo Levodopa ah 5 mLof dilute hydrochloric acid, oo ku samee tijaabada xalkan sida xalka tijaabada (aan ka badnayn 2 ppm).
(6) Walxaha laxidhiidha-Mila 0.10 g ee Levodopa gudaha 10 mL ee sodium disulfite TS, oo u isticmaal xalkan sida xalka muunada.Pipet 1 mL ee xalka muunada, ku dar sodium disulfite TS si aad u samayso 25 mL.Pipet 1 mL ee xalkan, ku dar sodium disulfite TS si aad u samayso 20 ml oo sax ah, una isticmaal xalkan sida xalka caadiga ah.Ku samee tijaabada xalalkan sida lagu farayo Chro-matography-ka khafiifka ah<2.03>.Bar 5mL mid kasta oo ka mid ah muunadda muunada iyo xalalka caadiga ah saxan cellulose ah oo loogu talagalay dhuuban-lakabka-chromatography.Ku samee saxanka isku dar ah 1-butanol, biyo, acetic acid (100) iyo methanol (10:5:5:1) masaafo dhan ilaa 10 cm, oo hawo-qalaji saxanka.Si toos ah u daadi xal ninhydrin ah oo acetone ah (1 50kiiba) saxanka dushiisa oo kulayl 90 ℃ 10 daqiiqo: dhibco kale oo aan ka ahayn meesha ugu muhiimsan ee xalka muunada kama badna meesha xalka caadiga ah.
Luminta qallajinta <2.41> In aan ka badnayn 0.30% (1 g, 105 ℃, 3 saacadood).
Hadhaaga dabka <2.44> In aan ka badnayn 0.1% (1 g).
Assay Si sax ah u miisaan qiyaastii 0.3 g oo Levodopa ah, oo hore loo qalajiyey, ku milmi 3 mL of formic acid, ku dar 80 ml acetic acid (100), iyo titrate <2.50> oo leh 0.1 mol/L perchloric acid VS ilaa midabka xalku isbeddelayo. laga bilaabo guduud ilaa buluug-cagaaran ilaa cagaar (tusaale: 3 dhibcood oo crystalviolet TS).Samee go'aan maran, oo samee sixid kasta oo lagama maarmaan ah.
ml kasta oo ah 0.1 mol/L perchloric acid VS ＝19.72 mg ee C9H11NO4
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