L-(-)-Tyrosine CAS 60-18-4 (H-Tyr-OH) Assay 98.5~101.5% Warshada Tayada Sare

L-(-)-Tyrosine (H-Tyr-OH) (CAS: 60-18-4) AJI 97 Habka Imtixaanka
Aqoonsiga: Is barbar dhig nooca nuugista infrared-ka ee muunada iyo kan heerka caadiga ah ee habka saxanka bromide ee potassium.
Wareegga Gaarka ah [α]20/D: Muunad la qallajiyey, C=5, 1mol/L HCl
Xaaladda Xalka ( Gudbinta): 0.5g gudaha 10ml ee 1mol/L HCl, spectrophotometer, 430nm, 10mm dhumucda unugga.
Chloride (Cl): 0.7g, A-1, tixraac: 0.40ml ee 0.01mol/L HCl
Ammonium (NH4): B-2
Sulfate (SO4): 1.2g, (1), tixraac: 0.50ml ee 0.005mol/L H2SO4
Birta (Fe): 1.5g, (2), tixraac: 1.5ml ee Iron Std.(0.01mg/ml)
Biraha culus (Pb): 2.0g, (2), tixraac: 2.0ml ee Pb Std.(0.01mg/ml)
Arsenic (As2O3): 2.0g, (2), tixraac: 2.0ml ee As2O3 Std.
Asiidhyada Amino Asiidhyada kale: Ku milmaan ammonia R2 Tusaalaha Tijaabada: 50μg, F-6-a, xakamaynta: L-Tyr 0.25μg
Luminta qallajinta: at 105 ℃ 3 saacadood.
Hadhaaga dabka (Sulfated): AJI Test 13
Qiimaynta: Muunad qallalan, 180mg, (1), 6ml oo ah aashitada formic, 50ml ee glacial acetic acid, 0.1mol/L HCLO4 1ml=18.119mg C9H11NO3
Tijaabada pH: 0.02g gudaha 100ml xal aqueous ah oo buuxa

L-(-)-Tyrosine (H-Tyr-OH) (CAS: 60-18-4) Habka Tijaabada USP35
Qeexitaan
Tyrosine waxa ku jira NLT 98.5% iyo NMT 101.5% ee L-Tyrosine (C9H11NO3), oo lagu xisaabiyo hab qallalan.
AQOONSIGA
A. NUUXDA INFRAREED <197K>
ASSAY
HABKA
Tusaalaha: 180 mg oo Tyrosine ah
Madhan: Isku qas 6 ml oo ah aashitada formic iyo 50 ml oo ah glacial acetic acid.
Nidaamka Titrimetric
(Fiiri Titrimetry <541>)
Habka: Titration toos ah
Titrant: 0.1 N perchloric acid VS
Ogaanshaha barta dhamaadka: Potentiometric
Falanqaynta: Ku mil muunada 6 mL oo ah asiidh formic ah iyo 50mL oo ah glacial acetic acid, oo titrate la Titrant.Samee go'aanka maran.
Xisaabi boqolkiiba tyrosine (C9H11NO3) ee muunadda la qaaday:
Natiijadu = {[(VS-VB) x N x F]/W} x100
VS= mugga Titrant ee uu isticmaalo Tusaalaha (mL)
VB= mugga Titrant ee uu isticmaalo Bannaanka (mL)
N= Caadiga dhabta ah ee Titrant (mEq/ml)
F= Qodob u dhigma, 181.2 mg/mEq
W= Miisaanka Tusaalaha (mg)
Shuruudaha aqbalaadda: 98.5% ~ 101.5% ee salka qallalan
XANUUNKA
DABINTA HAA DHACAY <281>: NMT 0.4%
Kloride iyo sulfate, Chloride <221>
Xalka caadiga ah: 0.20ml oo ah 0.020 N hydrochloric acid
Tusaalaha: 0.35 g oo ah Tyrosine.[FIIRO GAAR AH-Haddii loo baahdo, milaan Tusaalaha adiga oo kuleyliya meel u dhow karkarinta oo ku dar 1 ml oo nitric acid ah.]
Shuruudaha aqbalaadda: NMT 0.04%
Kloride iyo sulfate, Sulfate <221>
Xalka caadiga ah: 0.50ml oo ah 0.020 N sulfuric acid
Tusaalaha: 1.2 g oo ah Tyrosine.[FIIRO GAAR AH-Haddii loo baahdo, milaan Tusaalaha adiga oo ku daraya 6 ml oo ah hydrochloric acid la badhxay.]
Shuruudaha aqbalaadda: NMT 0.04%
IRON <241>: NMT 30ppm [Xusuusin-Haddii loo baahdo, isticmaal 2 mL oo ah hydrochloric acid si aad u milmaan Tusaalaha.]
Biraha culus, Habka I <231>: NMT 15ppm
KALA XIRIIR
Xalka ammonia la qasay: Ku qas 16 ml oo ammonia ah oo la ururiyey biyo ilaa 100 ml.
Xalka ku habboonaanta nidaamka: Kala dir 10 mg ee USP L-Tyrosine RS iyo 10 mg ee USP L-Phenylalanine RS ee 1 mL ee xalinta ammonia la karkariyey, oo ku mil biyo ilaa 25.0 mL.
Xalka caadiga ah: Galaas ku habboon, ku mil tiro USP L-Tyrosine RS ah 1 mL oo ah xal ammonia la karkariyey.Ku qas biyo ilaa mugga si aad u hesho xal 0.05mg/mL.[FIIRO GAAR AH-Xalkani waxa uu leeyahay fiirsi u dhigma 0.5% ee xalka Tusaalaha.]
Xalka muunada: Ku wareeji 0.1 g oo Tyrosine ah galaas mug leh oo 10-mL ah, ku dar xal ammonia ah oo ku filan si ay u milaan, oo biyo ugu qas.
Nadaamka Chromatographic (Eeg Chromatography <621>, Chromatography ee khafiifka ah.)
Habka: TLC
Adsorbent: 0.25-mm lakabka silica jel chromatographic ah
Codsiga mugga: 5μL
Horumarinta habka dareeraha: isopropyl khamriga iyo ammonium hydroxide (7: 3)
Daawada reagent: 2 mg/mL oo ninhydrin ah oo isku dhafka alkohol butyl ah iyo 2N acetic acid (95:5)
Ku habboonaanta nidaamka
Shuruudaha ku habboonaanta: chromatogram-ka xalka ku habboonaanta nidaamka wuxuu muujinayaa laba baro oo si cad u kala soocan.
Falanqaynta
Tusaalooyinka: Xalka ku habboonaanta nidaamka, xalalka caadiga ah, iyo xal muunad.
Qalaji saxanka inta u dhaxaysa 100 ° iyo 105° ilaa ammonia ay gebi ahaanba ka baaba'do.Ku buufin reagent buufin, oo kuleyl u dhexeeya 100 ° iyo 105 ° 15 daqiiqo.Ku baadh saxanka hoostiisa iftiin cad.
Shuruudaha aqbalaadda: Meel kasta oo labaad ee xalka Tusaalaha kama weyna ama kama xoog badna meesha ugu muhiimsan ee xalinta caadiga ah.
Wasakh kasta oo Shakhsiyeed: NMT 0.5%
Wadarta wasakhda: NMT 2.0%
IMTIXAANO GAAR AH
Wareegga indhaha, wareeg gaar ah <781S>
Xalka muunada: 50 mg/ml ee 1 N hydrochloric acid
Shuruudaha aqbalaadda: -9.8° ilaa -11.2°
QASAARAN QALABKA <731>: Ku qalaji muunad 105 ℃ 3 saac: waxay luminaysaa NMT 0.3% miisaankeeda.
SHURUUD DHEERAAD AH
Baakadaha iyo Kaydinta: Ku kaydi weelasha si fiican u xidhan.
HEERARKA Tixraaca USP <11>
USP L-Phenylalanine RS
USP L-Tyrosine RS