Phthalimide Potassium Salt CAS 1074-82-4 daahirnimo>99.0% (HPLC)

3. Habka tijaabada
3.1 Khasaare xagga qallajinta (W/W)
3.1.1 Qalab iyo qalab
In ka badan toban kun oo dheellitiran oo elektaroonig ah, foorno heerkulbeeg koronto ah, 40*25 dhalo miisaan leh, qalajiyaha
Reagent: jel silica
3.1.2 Hababka
1. Nadiifi 3 40*25 dhalooyinka miisaanka leh.
2, ku rid foornada at 105 ℃ (± 2 ℃) qallaji 1 saac.
3. Soo saar dhalada miisaanka 40*25 oo ku rid qalajiyaha si aad u qaboojiso 30min.
4, Saxan miisaankeedu yahay 40*25 dhalo cabbiraysa miisaan madhan W0.
5. Isticmaal dhalo miisaankeedu yahay 40*25 si aad u miisaanto muunada siman ee isku dhafan ee 3.0000g (W1), wadar ahaan 3.
6, Ku rid foornada, ka qaad fur dhalada, dhig agteeda dhalada miisaanka, ama kala badh fur furka dhalada si aad u qallajiso, soo bixi, waa inuu ahaadaa dabool dhalada miisaankeedu fiican yahay, qallaji 105 ℃ (± 2℃) 3 saacadood.
7. Ka soo saar dhalada miisaanka 40*25 oo ku rid qalabka qalajinta si aad u qaboojiso 30min.
8, Miisaan sax ah kadib qalajinta 40*25 miisaanka dhalada W2.
Qaacidada xisaabinta: Luminta qallajinta (%) = (W1-W2)/(W1-W0)×100%
3. 4 Go'aaminta nuxurka
3.4.1 Qalabka iyo qalabka
LC-UV100 Chromatograph dareere ah (Shanghai Wufeng Scientific Instrument Co., LTD.)
10ml dhalada volumetric, qaaddo geeska birta ah iyo tas-hiilaadka kale ee la xiriira
3.3.2 Hababka
Habka loo falanqeeyo ee Phthalimide Potassium Salt
3.3.3 chromatography dareere ah
Xaaladda Chromatographic
Tiirka Chromatographic: HypersilC6H5 (C8 sidoo kale waa la heli karaa) 4.6*250mm 5um
Dhererka hirarka: 210nm
Heerkulka tiirka: 30℃
Heerka socodka: 1.0ml/min
Muunad uruurinta: 0.05mg/ml
Reagents
Potassium Phosphate Dibasic (analytical saafi ah)
Phthalimide Potassium Salt ( muunadda caadiga ah)
Acetonitrile (analytical saafi ah)
Biyo saafi ah
Marxaladda wareegta: acetonitrile: phosphate buffer (PH:3.5) =40:60
Miisaanka 1.63g Potassium Phosphate Dibasic iyo milaan 600ml biyo saafi ah, hagaajin qiimaha PH ilaa 3.5 leh fosfooraska acid, ku shub 1000ml beaker ka kooban 400ml acetonitrile, si fiican u qas, filter leh 0.45um isku qasan xuubka filter fiber, milmi leh gariir ultrasonic. codsi.
3.3.4 Habraaca
1) Xalka tijaabada qaabaynta (0.05mg/ml)
Muunadda 2.5mg ayaa si sax ah loo miisaamay waxaana lagu riday dhalada mugga 50ml ah.Marxaladda guurguura ayaa lagu daray oo lagu qasi jiray cabbirka kala dirida ultrasonic.
2) Qaabaynta xalalka caadiga ah (1.0mg/ml)
Muunadda caadiga ah ee 2.5mg ayaa si sax ah loo miisaamay waxaana lagu riday dhalada mugga 50ml.Marxaladda guurguura ayaa lagu daray oo lagu qasi jiray cabbirka kala dirida ultrasonic.
3) Isticmaalka nidaamka:
(1) Madaxa shaandhada geli 80% methanol marka la bilaabayo, fur waalka qiiqa ka dibna daadi 3-5min, oo xidh shafka qiiqa oo biyo raaci 10-15min.Kadibna madaxa shaandhada ku rid 90% biyo saafi ah, fur furka biyaha daadi oo daadi 3-5min, xidh shafka oo biyo raaci 10min.
(2) Kadib beddel wejiga moobilka ee la diyaariyey, heerka socodka qulqulku waa 1.0ml/min, cadaadiska hore ee tiirka wuxuu ka yar yahay 1000Psi, fur qalabka wax baadha, sug ilaa cadaadiska hore ee tiirarka uu xasilloon yahay, fur goobta shaqada kombiyuutarka, oo gal online
(3) Ka dib markii cadaadiska hore ee tiirka uu xasilloon yahay, fur qalabka wax baaraha.Marka hore ku dur 1 irbad oo wareega wareega ah, ka dibna ku dur 1 irbad muunada caadiga ah, oo ugu dambayn isku duri muunad.
(4) Natiijooyinka xisaabinta: habka caadiga ah ee aagga
(5) Kadib markaad dhammayso hawsha falanqaynta, xidh baaraha marka hore, ka dibna ku raaci madaxa shaandhada biyo saafi ah 10-15min;Isla mar ahaantaana, tuubada duritaanka waxaa lagu dhaqay biyo iyo methanol.
(6) Ugu dambayntii, xidho 80% methanol, biyo raaci 30min, oo xidh.
4. Kadib dhamaystirka baadhista, buuxi xaashida baadhista iyo shaybaadhka.