Chenodeoxycholic Acid (CDCA) CAS 474-25-9 Assay ≥98% (Dry Basic)

Chenodeoxycholic acid
C24H40O4: 392.57
3a,7a-Dihydroxy-5b-cholan-24-oic acid [474-25-9]
Chenodeoxycholic Acid, ha e omisitsoe, ha e na ka tlase ho 98.0z le ho feta 101.0% ea C24H40O4.
Tlhaloso Chenodeoxycholic Acid e hlaha e le tšoeu, likristale, phofo ea crystalline kapa phofo.
E qhibiliha ka bolokolohi ho methanol le ethanol (99.5), e qhibilihang ka har'a acetone, 'me e batla e sa qhibilihe ka metsing.
Identication Netefatsa infrared absorption spectrum ea Chenodeoxycholic Acid, e neng e omisitsoe pele, joalo ka ha e laetsoe ka mokhoa oa potassium bromide disk tlas'a Infrared Spectrophotometry <2.25>, 'me u bapise spectrum le Reference Spectrum: ka bobeli li bonts'a matla a tšoanang a ho monya ka linomoro tse tšoanang tsa maqhubu.
Optical rotation <2.49> [a]20D : +11.0 °~ +13.0 ° (kamora ho omisa, 0.4 g, ethanol (99.5), 20 mL, 100 mm).
Sebaka sa ho qhibiliha <2.60> 164 ~ 169 ℃
Bohloeki (1) Chloride <1.03>-Tlosa 0.36 g ea Chenodeoxycholic Acid ka 30 ml ea methanol, eketsa 10 ml ea dilute nitric acid le metsi ho etsa 50 mL, 'me u etse tlhahlobo ka tharollo ena.Lokisetsa tharollo ea taolo ka tsela e latelang: Ho 1.0 mL ea 0.01 mol/L hydrochloric acid VS eketsa 30 mL ea methanol, 10 mL ea dilute nitric acid le metsi ho etsa 50 mL (eseng ho feta 0.1%).
(2) Litšepe tse boima <1.07> -Tsoela pele ka 1.0 g ea Chenodeoxycholic Acid ho ea ka Method 4, 'me u etse tlhahlobo.Lokisetsa tharollo ea taolo ka 2.0 mL ea Standard Lead Solution (eseng ho feta 20 ppm).
(3) Barium-Ho ea ho 2.0 g ea Chenodeoxycholic Acid eketsa 100 ml ea metsi, 'me u belise metsotso e 2.Ho tharollo ena eketsa 2 mL ea hydrochloric acid, pheha metsotso e 2, sefa ka mor'a ho pholile, 'me u hlatsoe sefe ka metsi ho fihlela u fumana 100 ml ea filtrate.Ho 10 ml ea filtrate eketsa 1 mL ea dilute sulfuric acid: ha ho turbidity e hlahang.
(4) Lintho tse amanang le tsona-Hlakola 0.20 g ea Chenodeoxycholic Acid ka motsoako oa acetone le metsi (9: 1) ho etsa 10 ml hantle, 'me u sebelise tharollo ena e le mohlala oa tharollo.Ka thoko, qhala 10 mg ea lithocholic acid bakeng sa chromatography e tšesaane ka har'a motsoako oa acetone le metsi (9: 1) ho etsa 10 ml hantle.Pipet 2 ml ea tharollo ena, eketsa motsoako oa acetone le metsi (9: 1) ho etsa 100 ml hantle, 'me u sebelise tharollo ena e le tharollo e tloaelehileng (1).Ka thoko, qhala 10 mg ea ursodeoxycholic acid ka har'a motsoako oa acetone le metsi (9: 1) ho etsa 100 ml hantle, 'me u sebelise tharollo ena e le tharollo e tloaelehileng (2).Ka thoko, qhala 10 mg ea cholic acid bakeng sa chromatography e tšesaane ka har'a motsoako oa acetone le metsi (9: 1) ho etsa 100 ml hantle, 'me u sebelise tharollo ena e le tharollo e tloaelehileng (3).Pipe 1 ml ea tharollo ea sampole, 'me u kenye motsoako oa acetone le metsi (9:1) ho etsa 20 ml hantle.Pipete 0.5 mL, 1 mL, 2 mL, 3 mL le 5 ml ea tharollo ena, eketsa motsoako oa acetone le metsi (1) ho e 'ngoe le e' ngoe ea tsona ho etsa 50 ml hantle, 'me u khethe litharollo tsena e le tharollo e tloaelehileng ea A, tharollo e tloaelehileng. B, tharollo e tloaelehileng C, tharollo e tloaelehileng D le tharollo e tloaelehileng E, ka ho latellana.Etsa tlhahlobo ka litharollo tsena joalokaha ho laetsoe tlas'a Thin-layer Chromatography<2.03>.Spot 5 ml e 'ngoe le e' ngoe ea tharollo ea mohlala, litharollo tse tloaelehileng (1), (2), (3) le litharollo tse tloaelehileng A, B, C, D le E holim'a poleiti ea silica gel bakeng sa chromatography ea lera le tšesaane.Theha poleiti ka motsoako oa 4-methyl-2-pentanone, toluene le formic acid (16: 6: 1) ho isa bohōle ba lisenthimithara tse 15, omisa poleiti, 'me o omelle ho 1209C ka metsotso e 30.Hang-hang, fafatsa ka ho lekana tharollo ea phosphomolybdic acid n-hydrate ka ethanol (95) (1 ho 5) poleiti, 'me mocheso ka 120 ℃ metsotso e 2 ho isa ho e 3: sebaka se tsamaisanang le sebaka se nang le tharollo e tloaelehileng (1) ha le matla ho feta letheba le nang le tharollo e tloaelehileng (1), letheba le lumellanang le letheba le nang le tharollo e tloaelehileng (2) ha le matla ho feta letheba le nang le tharollo e tloaelehileng (2), le letheba le tsamaellanang le letheba. ka tharollo e tloaelehileng (3) ha e matla ho feta sebaka se nang le tharollo e tloaelehileng
(3).Ha ho bapisoa le matheba a nang le litharollo tse tloaelehileng A, B, C, D le E, matheba ntle le sebaka se ka sehloohong le tse ling ntle le matheba a boletsoeng ka holimo ha a matla ho feta sebaka se nang le tharollo e tloaelehileng ea E, le kakaretso ea chelete. ea bona ha e fete 1.5%.
Tahlehelo ha o omisa <2.41> E seng ho feta 1.5%(1 g, 105℃,3 lihora).
Masalla ha a khantšitsoe <2.44> Ha a fete 0.1% (1 g).
Assay Beha boima bo nepahetseng ka 0.5 g ea Chenodeoxycholic Acid, e neng e omisitsoe pele, qhala ka 40 mL ea ethanol (95) le 20 mL ea metsi, le titrate <2.50> le 0.1 mol/L sodium hydroxide VS (potentiometric titration).Etsa qeto e se nang letho ka mokhoa o ts'oanang, 'me u etse tokiso e hlokahalang.
ML ka 'ngoe ea 0.1 mol/L sodium hydroxide VS ＝ 39.26 mg ea C24H40O4
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