Flurbiprofen CAS 5104-49-4 Assay 99.0~100.5% (HPLC) Factory

Flurbiprofen
C15H13FO2 244.27
(±) -2-(2-Fluoro-4-biphenylyl) propionic acid [5104-49-4].
Flurbiprofen ha e na ka tlaase ho karolo ea 99.0 lekholong le e seng ho feta 100.5 lekholong ea C15H13FO2, e baloang motheong o omisitsoeng.
Ho paka le ho boloka- Boloka ka lijaneng tse thata.
USP Reference standards <11>-
USP Flurbiprofen RS
USP Flurbiprofen Related Compound A RS
2-(4-Biphenylyl) propionic acid.
C15H14O2 226.28
Boitsebiso-
A: Infrared Absorption <197K>.
B: Ho monya ha Ultraviolet 197U-
Tharollo: 10 µg ka mL.
Mahareng: 0.1 N sodium hydroxide.
Absorbance boholo ho 247 nm ke hoo e ka bang 0.8.
Sebaka sa ho qhibiliha <741>: pakeng tsa 114 le 117.
Tahlehelo ka ho omisa <731> -E omise ka vacuum ho 60 ho boima bo sa feleng: ha e lahlehe ho feta 0.5% ea boima ba eona.
Masala a ho khantša <281>: eseng ho feta 0.1%.
Litšepe tse boima, Mokhoa oa II <231>: 0,001%.
Likopano tse amanang-
Diluent-Lokisetsa motsoako oa metsi le acetonitrile (11: 9).
Mobile phase-Lokisetsa motsoako o sefiloeng le o sentsoeng oa metsi, acetonitrile, le glacial acetic acid (12:7:1).Etsa litokiso ha ho hlokahala (sheba Tšebeliso ea Sistimi tlas'a Chromatography <621>).
Standard stock solution- Hlakola boima bo lekantsoeng bo nepahetseng baUSP Flurbiprofen Related Compound A RS in Diluent ho fumana tharollo e nang le mahloriso a ka bang 50 µg ka mL.
Tharollo ea ho tshwaneleha ha Sistimi-Pipet 2.0 mL of Standard stock solution ka har'a botlolo ea volumetric ea 10-mL, eketsa hoo e ka bang 20 mg ea USP Flurbiprofen RS, hlapolla ka Diluent ho bophahamo, 'me u kopanye.
Tharollo e tloaelehileng-Fetisetsa 2.0 mL ea tharollo ea Setoko se Tloaelehileng ho botlolo ea volumetric ea 10.0-mL, hlapolla ka Diluent ho bophahamo, 'me u kopanye.
Tharollo ea teko-Lokisetsa tharollo ea Flurbiprofen ka Diluent e nang le 2.0 mg ka mL.
Chromatographic system (sheba Chromatography <621>)-Mokelikeli oa chromatograph o na le mochine oa 254-nm le kholomo ea 3.9-mm × 15-cm e nang le 4-µm packing L1.Sekhahla sa phallo ke hoo e ka bang 1 mL ka motsotso.Chromatograph the System suitability solution, le ho tlaleha likarabelo tse phahameng joalo ka ha li laetsoe Ts'ebetso: nako e lekanyelitsoeng ea ho boloka e ka ba 0.9 bakeng sa motsoako o amanang le Flurbiprofen A le 1.0 bakeng sa flurbiprofen;'me kheloha e lekanyelitsoeng bakeng sa liente tse ikatisang ha e fete 1.0%.
Ts'ebetso-Ka karohano kenya meqolo e lekanang (hoo e ka bang 20 µL) ea Tharollo e Tloaelehileng le Tharollo ea Teko ka har'a chromatograph, rekota li-chromatogram, 'me u lekanye libaka tsa litlhoro tse kholo.Bala peresente ea motsoako o amanang le flurbiprofen A karolong ea Flurbiprofen e nkiloeng ka foromo:
100(CS / CU)(rU / rS)
moo CS e leng mahloriso, ka µg ka mL, ea USP Flurbiprofen Related Compound A RS ka tharollo ea Standard;CU ke mahloriso, ho µg ka mL, ea Flurbiprofen tharollong ea Teko;'me rU le rS ke likarabelo tse phahameng ka ho fetisisa bakeng sa motsoako o amanang le flurbiprofen A o fumanoang ho tsoa ho Tharollo ea Teko le Tharollo ea Standard, ka ho latellana: ha ho na ho feta 0.5% ea motsoako oa Flurbiprofen o fumanoang.Bala liperesente tsa ho se hloeke ka 'ngoe karolong ea Flurbiprofen e nkiloeng ka foromo:
100(ri / rs)
eo ho eona ri e leng karabelo ea tlhoro bakeng sa ho se hloeke ka 'ngoe e fumanoang ho tsoa ho Tharollo ea Tlhahlobo;'me rs ke kakaretso ea likarabelo tsa litlhōrō tsohle tse fumanoeng Tharollong ea Teko: kakaretso ea litšila tsohle ha e fete 1.0%.
Assay-Dissolve e ka bang 0.5 g ea Flurbiprofen, e lekantsoeng hantle, ka 100 ml ea joala, eo pele e neng e sa sebetse ka 0.1 N sodium hydroxide VS ho phenolphthalein endpoint, eketsa phenolphthalein TS, le titrate ka 0.1 N sodium hydroxide VS ponahalo e fokolang ea pele. 'mala o pinki o tšoarellang nako e seng ka tlase ho metsotsoana e 30.ML e 'ngoe le e' ngoe ea 0.1 N sodium hydroxide e lekana le 24.43 mg ea C15H13FO2.