Glycine CAS 56-40-6 (H-Gly-OH) Assay 98.5 ~ 101.5% Factory High Quality

USP35-NF30
TLHALOSO
Glycine e na le NLT 98.5% le NMT 101.5% ea glycine (C2H5NO2), e baloang ka mokhoa o omisitsoeng.
TS'ELISO TS'OANE
A. HO AMOHELA INFRRARED <197M>
TLHOKOMELO
• TSAMAISO
Mohlala: 150 mg ea Glycine
Sekheo: 100 ml ea glacial acetic acid
Sistimi ea Titrimetric
(Sheba Titrimetry <541>.)
Mokhoa: Titration e tobileng
Titrant: 0.1 N ka chloric acid VS
Ho lemoha ha ntlha ea ho qetela: Ho bonoa
Tlhahlobo: Qhala Mohlala ka 100 mL ea glacial acetic acid, 'me u kenye lerotholi le le leng la crystal violet TS.Titrate le Titrant ho ea qetellong e tala.Etsa boitlamo bo se nang letho.
Bala liperesente tsa glycine (C2H5NO2) ho Mohlala o nkiloeng:
Sephetho = {[(VS − VB) × N × F]/W} × 100
VS = Bophahamo ba Titrant bo jeoang ke Mohlala (mL)
VB = Bolumo ba Titrant bo jeoang ke Blank (mL)
N = tloaelo ea 'nete ea Titrant (mEq/mL)
F = ntlha ea ho lekana, 75.07 mg/mEq
W = Boima ba mohlala (mg)
Mekhoa ea ho amohela: 98.5% -101.5% motheong o omisitsoeng
LITŠILA
• MASALE A THUSANG <281>: NMT 0.1%
• CHLORIDE LE SULFATE, Chloride <221>
Tharollo e tloaelehileng: 0.10 mL ea 0.020 N hydrochloric acid
Mohlala: 1 g ea Glycine
Maemo a kamohelo: NMT 0.007%
• CHLORIDE LE SULFATE, Sulfate <221>
Tharollo e tloaelehileng: 0,20 mL ea 0.020 N sulfuric acid
Mohlala: 3 g ea Glycine
Maemo a kamohelo: NMT 0.0065%
• LITŠEPE TSE BOHLOKO, Mokhoa oa I <231>: NMT 20 ppm
• LITŠEPE TSE BOHLOKO, Mokhoa oa I <231>: NMT 20 ppm
Mohlala oa tharollo: 100 mg/mL ea Glycine
Tlhahlobo: Belisa 10 ml ea tharollo ea Mohlala bakeng sa 1 min, 'me u behelle ka thōko bakeng sa 2 h.
Mekhoa ea ho amohela: Tharollo e bonahala e hlakile ebile e le e tsamaeang joaloka 10 mL ea tharollo e tšoanang e sa phehiloeng.
LITEKO TSEKO
• TAHLEHELO KA HO OMETSA <731>: Omisa sampole ho 105° bakeng sa 2 h: e lahleheloa ke NMT 0.2% ea boima ba eona.
KEKELETSO LIHLOKO
• PAKARETSO LE HO BOLOKA: Boloka ka lijaneng tse koetsoeng hantle.
• LITEKELO TSA USP REFERENCE <11>
USP Glycine RS

Pharmacopoeia ea Japane JP17
Glycine, ha e omisitsoe, ha e na ka tlase ho 98.5% ea Glycine(C2H5NO2).
Tlhaloso Glycine e hlaha e le tšoeu, likristale kapa phofo ea crystalline.E na le tatso e monate.E qhibiliha ka bolokolohi metsing le ka asiti ea formic, 'me e batla e sa qhibilihe ka har'a ethanol (95).E bontša kristale polymorphism.
Identication Lekola sekhahla sa ho monya ha Glycine, se neng se omisitsoe pele, joalo ka ha se laetsoe ka mokhoa oa potassiumbromide disk tlas'a Infrared Spectrophotometry <2.25>, 'me u bapise spectrum le Reference Spec-trum: ka bobeli li bonts'a matla a ts'oanang a ho monya ka linomoro tse tšoanang tsa maqhubu.Haeba ho na le phapang e teng pakeng tsa spectra, qhala Glycine ka metsing, e etsa mouoane ho omella ha metsi, 'me u phete tlhahlobo le masalla.
pH <2.54> Qhala 1.0 g ya Glycine ka 20 mL ya metsi: thepH ya tharollo e pakeng tsa 5.6 le 6.6.
Bohloeki
(1) Ho hlaka le 'mala oa tharollo-Hlakola 1.0 gof Glycine ka 10 mL ea metsi: tharollo e hlakile ebile ha e na mebala.
(2) Chloride <1.03> -Etsa tlhahlobo ka 0.5 g ea Glycine.Lokisetsa tharollo ea taolo ka 0.30 mL ea 0.01mol/L hydrochloric acid VS (e seng ho feta 0.021%).
(3) Sulfate <1.14> -Etsa tlhahlobo ka 0.6 g ea Glycine.Lokisetsa tharollo ea taolo ka 0.35 mL ea 0.005mol/L sulfuric acid VS (e seng ho feta 0.028z).
(4) Ammonium <1.02> -Etsa tlhahlobo ho sebelisa 0.25 g ea Glycine.Lokisetsa tharollo ea taolo ka 5.0 mL ea Standard Ammonium Solution (e seng ho feta 0.02%).
(5) Metals e boima <1.07> -Tsoela pele ka 1.0 g ea Glycine ho ea ka Mokhoa oa 1, 'me u etse tlhahlobo.Lokisetsa tharollo ea taolo ka 2.0 mL ea Standard Lead Solution (eseng ho feta 20 ppm).
(6) Arsenic <1.11> -Lokisetsa tharollo ea teko le 1.0 gof Glycine ho latela Method 1, 'me u etse tlhahlobo (eseng ho feta 2 ppm).
(7) Lintho Tse Amanang-Tlosa 0.10 g ea Glycine ka 25mL ea metsi 'me u sebelise tharollo ena e le mohlala oa tharollo.Pipete 1 ml ea tharollo ea sampole, eketsa metsi ho etsa 50 ml hantle.Pipet 5 mL ea tharollo ena, eketsa metsi ho etsa hantle 20 mL, 'me u sebelise tharollo ena e le tharollo e tloaelehileng.Etsa tlhahlobo ka litharollo tsena joalokaha ho laetsoe tlas'a Thin-layer Chromatography<2.03>.Spot 5mL e 'ngoe le e 'ngoe ea tharollo ea sampole le tharollo e tloaelehileng poleiting ea silika gel forthin-layer chromatography.Theha poleiti ka motsoako oa 1-butanol, metsi le acetic acid (100) (3: 1: 1) ho ea bohōle ba 10 cm, 'me u omise poleiti ka 80 ℃ metsotso e 30.Fafatsa ka ho lekana tharollo ea ninhydrin ka har'a acetone (1 ho 50), 'me mocheso ho 80 ℃ metsotso e 5: matheba ntle le letheba la mantlha ho tsoa ho tharollo ea sampole ha a matla ho feta sebaka se tsoang tharollong e tloaelehileng.
Tahlehelo ka ho Omisa <2.41> Ha e fete 0.30% (1 g, 105℃, lihora tse 3).
Masalla a ho Ignition <2.44> Ha a fete 0.10% (1g).
Assay Beha boima bo nepahetseng ka 80 mg ea Glycine, e neng e omisitsoe pele, qhala ka 3 mL ea formic acid, eketsa 50 mL ea acetic acid (100), le titrate <2.50> ka 0.1 mol/L perchloric acid VS (potentiometric titration).Etsa qeto e se nang letho, 'me u etse tokiso e hlokahalang.
ML ka 'ngoe ea 0.1 mol/L perchloric acid VS＝7.507 mg ea C2H5NO2
Lijana le polokelo Lijana-Lits'oants'o tse koetsoeng hantle.