H-Glu(OEt)-OEt·HCl CAS 1118-89-4 L-Glutamic Acid Diethyl Ester Hydrochloride Purity >99.0% (HPLC) Factory

I. Mekhoa ea ho hlahloba
1. Ntho e qhibilihang
Ho sebelisoa sesebelisoa sa digital melting point
Mohato oa 2: Litaba
2.1 Li-reagents
Mohato oa 1: Formic acid
2. Glacial acetic acid
3. Perchloric acid e tloaelehileng ea titration solution (C=0.1000mol/l)
4. Mokelikeli oa Crystal Violet indicator: 5g / l.
2.2 Lisebelisoa le lisebelisoa
1. Beaker 150ml
2. Microburet: 10ml
2. 3 Mehato ea tlhahlobo
2. 3. Ke lekanya ka nepo ka 300mg ea sampuli e omisitsoeng pele ho 105 ℃ bakeng sa 3h, e kenye ka bethel ea 150ml, eketsa 3ml ea formic acid, eketsa 50ml ea ice acetic acid, eketsa marotholi a 2 a crystal violet test solution ( TS-74), le titrate ka 0.1mol/L perchloric acid ho fihlela ntlha e tala kapa ho fihlela boputsoa bo nyamela ka ho felletseng.
2. 3. 2 Mokhoa oa ho bala
X e lekana le C (V1-V2) makhetlo a 0.2042 / M makhetlo a 100
C ke motsoako oa perchloric acid standard titration solution, e hlahisoang ka moles ka litha e le nngwe (mol/l).
V1 ke boleng bo sebelisitsoeng ba perchloric acid standard titration concentrated solution ho sampole titration, e hlahisoang ka ml (ml).
Bolumo bo sebelisitsoeng ba perchloric acid standard titration solution tekong e se nang letho ea V2, e hlahisoang ka ml (ml)
0.2042 le 1.00ml perchloric acid standard titrated concentrated liquid phase ha boleng ba sehlahisoa sena bo hlahisoa ka G.
M Boleng ba sampole, bo hlahisitsoeng ka ligrama (g).
2. 4 Khalemelo
Haeba phapang ea mocheso oa kamore pakeng tsa tharollo ea teko ea titration le tharollo e tloaelehileng ea perchloric acid e feta 10 ℃, e lokela ho lekanngoa hape;Ho seng joalo, khatello ea C1 ea tharollo e tloaelehileng ea perchloric acid e tla lokisoa ho latela tlhahlobo (2).
C2= C1/1+0.0011 (t1-t2)
1+0.0011 ke coefficient ea katoloso ea glacial acetic acid
Karolo ea mocheso oa kamore bakeng sa tekano ea T2 ea perchloric acid ke Celsius (° C).
Karolo ea mocheso oa kamore eo sampole e titrated ke Celsius (° C).
C1 t2℃ perchloric acid standard solution concentration, in moles per litha (MOL/L)
3. E omme ebile e se na boima
3.1 Lisebelisoa:
Thermostatic drying ontong, tekanyo ea 1/10,000.
3.2 Mokhoa:
Nka ligrama tse 1-2 tsa disampole (tse nepahetseng ho isa ho 0.5mg) 'me u li kenye ka har'a botlolo ea boima ba 'mele e omisitsoeng pele le e bekhang.Mehlala e tla ba le botenya bo ts'oanang botlaaseng ba botlolo ea boima.Ha e le lisampole, li tla omisoa ka ontong ea thermoelectric (105±5℃) ho isa boima bo sa feleng.
3.3 Palo
X ke m minus m1 e arotsoe ka m minus m2 makhetlo a 100
Moo: Mongobo oa X%
m1 -- Boima ba sampole e ommeng hammoho le botlolo ea ho metha g
m2 - Lekanya boima ba botlolo g
m- Boima ba sampole g
3.4 Lintlha tsa Tlhokomeliso
1. Lintlha tsa tlhokomelo: 1. Li-samples tse tšoanang li tlameha ho etsoa bakeng sa ho omisa le ho hloka boima.Mohlala o le mong o na le liphetho tse peli, 'me phoso pakeng tsa liphetho tse peli ha e fete 0.5%.
2. Boima bo tlameha ho ba hlokolosi, bo nepahale ho 0.0001.
4 ho potoloha
4.1 Melao-motheo
Leseli la sefofane le fetang har'a mokelikeli kapa tharollo ea motsoako o nang le ts'ebetso e itseng ea optical (ke hore, ntho e sebetsang ka mokhoa oa optical) e ka baka ho potoloha ha leseli, ho etsa hore sefofane sa polarized light se potoloha ka ho le letšehali kapa ho le letona.Palo ea likhato tsa ho potoloha e bitsoa optical rotation.Mochesong o itseng (hangata o hlahisoang ke t, e ka ba 20℃ kapa 25℃) khanya e itseng ea maqhubu (leseli le mosehla la sodium le D, wavelength λ 589.3nm) leseli le polarized ka milliliter e 'ngoe le e' ngoe e nang le 1g ea lisebelisoa tsa optical, botenya ba 1dm. (ke hore, 10cm) tharollo ha ho potoloha ho bitsoa "special optical rotation".
4.2 Litlhoko
Mohala oa D oa sodium spectrum (589. 3 nm) o ne o sebelisetsoa ho lekanya bolelele ba tube ea 1dm mme mocheso o ne o le 20 ℃.
4.3 Lisebelisoa
4.3.1 Digital autotropy (ho nepahala ±0.001°)
4.3.2 Tekanyo ea elektronike (ho nepahala 0.0001g).
4.4 Mokhoa oa Ts'ebetso
Nka 3g ea sehlahisoa sena, e nepahetseng ho 0.0001g, e kenye ka botlolo ea 100ml ea volumetric, eketsa ethanol ho qhaqha le ho e hlapolla ka tekanyo, sisinya hantle.Hlatsoa tšupu ea ho metha ka mokelikeli o monyenyane ka makhetlo a 'maloa, ebe butle-butle kenya mokelikeli ka har'a tube ea ho metha (u se ke ua etsa hore li-bubble li hlahe), beha tube ea ho metha ka har'a gyroscope bakeng sa ho fumanoa, 'me u bale ho bala ho potoloha ha mahlo. 3 makhetlo a bapileng.
4.5 Palo
Potoloho e khethehileng ea L-glutamate Diethyl carbonate ho sodium spectrum D-line ho 20 ℃ [Alpha]D20 e baloa ka tsela e latelang:
[ɑ] D20 = alpha/m
Moo: α -- palo e tloaelehileng ea palo ea li-cubic rotation;
m -- mohlala oa boleng.
4.6 Phapang e amohelehang: Phapang e feletseng ea sampuli e tšoanang e ke ke ea feta 0.02% ha e lekanngoa habeli.
4.7 Litlhokomelo
4.7.1 Solvent e lokela ho sebelisoa e le tekanyo e se nang letho pele ho tekanyo e 'ngoe le e 'ngoe: Ka mor'a ho lekanya, tekanyo e lokela ho etsoa hape ho fumana hore na lintlha tsa zero lia fetoha nakong ea tekanyo.Haeba ho na le liphetoho leha e le life, ho potoloha ha optical ea sampuli ho lokela ho khethoa hape.
4.7.2 Mohlala o lokela ho hlakisoa ka mor'a ho qhibiliha le ho hlapolloa;ho seng joalo, e lokela ho sefshoa.
Mohato oa 5 Ponahalo
Sehlahisoa se tla ba White to off-white microcrystalline powder