Histamine Dihydrochloride CAS 56-92-8 Assay 98.5~101.0% (Titration) Factory

Histamine Dihydrochloride (CAS: 56-92-8) EP9.0 MokhoaⅡMokhoa oa Teko
TLHALOSO
Histamine dihydrochloride e na le liperesente tse seng ka tlase ho 98.5 mme ha e fete liperesente tse 101.0 tsa 2-(1H-imidazol-4-yl) ethan-1-amine dihydrochloride, e baloang ho ipapisitsoe le ntho e omisitsoeng.
LIEKETSENG
Phofo e tšoeu kapa e batlang e le tšoeu, kristale kapa likristale tse se nang 'mala, hygroscopic, e qhibilihang haholo metsing, e qhibilihang joala.
TS'ELISO TS'OANE
Boitsebiso ba pele: A, D.
Boitsebiso ba bobeli: B, C, D
A. Hlahloba ka infrared absorption spectrophotometry (2.2.24), ho bapisa le spectrum e fumanoang ka histamine dihydrochloride CRS.Hlahloba e le li-discs tse lokiselitsoeng ho sebelisa 1 mg ea ntho.
B. Hlahloba li-chromatogram tse fumanoeng tekong ea histidine.Sebaka sa mantlha sa chromatogram se fumanoeng ka tharollo ea teko (b) se tšoana ka boemo, 'mala le boholo le sebaka sa mantlha sa chromatogram se fumanoeng ka tharollo ea litšupiso (a).
C. Qhala 0.1 g ho 7 mL ea metsi R 'me u kenye 3 ml ea tharollo ea 200 g/L ea sodium hydroxide R. Qhala 50mg ea sulfanilic acid R motsoako oa 0.1 mL ea hydrochloric acid R le 10 ml ea metsi R le eketsa 0.1 mL ea sodium nitrite tharollo R. Eketsa tharollo ea bobeli ho ea pele le ho kopanya.Ho hlahisoa 'mala o mofubelu.
D. E fana ka karabelo (a) ea chloride (2.3.1).
LITEKO
Tharollo S. Hlakola 0.5g metsing a se nang carbon dioxide R a lokiselitsoeng ho tloha metsing a hloekisitsoeng R le ho hlapolla ho 10 mL ka solvent e tšoanang.
Ponahalo ea tharollo.Tharollo S e hlakile (2.2.1) mme ha e na mebala e matla ho feta tharollo ea Y7 (2.2.2, Method II).
pH (2.2.3).PH ea tharollo S ke 2.85 ho 3.60.
Histidine.Hlahloba ka chromatography ea lera le tšesaane (2.2.27), u sebelisa TLC silica gel G plate R.
Tharollo ea teko (a).Qhala 0.5g ea ntho e lokelang ho hlahlojoa ka metsing R 'me u hlapolle ho 10mL ka solvent e tšoanang.
Tharollo ea teko (b).Hlakola 2 ml ea tharollo ea teko (a) ho 10 ml ka metsi R.
Tharollo ea litšupiso (a).Qhala 0.1 g ya histamine dihydrochloride CRS ka metsing R mme o hlapolle ho 10 mL ka solvent e tšoanang.
Tharollo ea litšupiso (b).Qhala 50 mg ea histidine monohydrochloride R ka metsing R 'me u hlapolle ho 100 ml ka solvent e tšoanang.
Tharollo ea litšupiso (c).Kopanya 1 mL ea tharollo ea teko (a) le 1 mL ea tharollo ea litšupiso (b).
Etsa kopo poleiting ea 1 μL ea tharollo ea teko (a), 1 μL ea tharollo ea teko (b), 1 μL ea tharollo ea litšupiso (a), 1 μL ea tharollo ea litšupiso (b) le 2 μL ea tharollo ea litšupiso (c).Hlahisa ka tsela ea 15cm ho sebelisa motsoako oa 5volumes ea concentrated ammonia R, 20 volume ea metsi R le 75 volume ea acetonitrile R. Omisa poleiti moeeng oa moea.Pheta nts'etsopele ka tsela e ts'oanang, omisa poleiti ka har'a moea 'me u fafatse ka tharollo ea ninhydrin R1.Chesa poleiti ka 110 ° C bakeng sa 10 min.Letheba lefe kapa lefe le amanang le histidine ka har'a chromatogram e fumanoeng ka tharollo ea tlhahlobo (a) ha le matla ho feta sebaka se ho chromatogram se fumanoeng ka tharollo ea litšupiso (b) (1 lekholong).Teko ha e sebetse ntle le haeba chromatogram e fumanoeng ka tharollo ea litšupiso (c) e bonts'a matheba a 2 a arohaneng ka ho hlaka.
Li-sulfate (2.4.13).3mL ea tharollo S e hlapolotsoeng ho 15 mL ka metsi a hloekisitsoeng R e lumellana le teko ea moeli bakeng sa sulfate (liperesente tse 0.1).
Tahlehelo ka ho omisa (2.2.32).Ha e fete liperesente tse 0.5, e ikemiselitseng ho 0.20 g ka ho omisa ka ontong ka 105 ° C.
Molora o sulfated (2.4.14).Ha e fete liperesente tse 0.1, e ikemiselitseng ho 0.5 g.
TLHOKOMELO
Qhala 0.080 g ka motsoako oa 5.0 mL ea 0.01 M hydrochloric acid le 50 mL ea joala R. Etsa potentiometric titration (2.2.20), sebelisa0.1 M sodium hydroxide.Bala molumo o kentsoeng pakeng tsa ntlha ea pele le ea boraro ea inflexion.
1mL ea 0.1 M sodium hydroxide e lekana le 9.203 mg ea C5H11Cl2N3.
POLOKO
Boloka ka har'a setshelo se sa keneleng moea, se sirelelitsoeng leseli.