L-4-Nitrophenylalanine Methyl Ester HCl CAS 17193-40-7 H-Phe(4-NO2)-OMe·HCl Assay >98.5% (HPLC) Factory

Mokhoa oa Tlhahlobo oa L-4-Nitrophenylalanine Methyl Ester HCl (H-Phe(4-NO2)-OMe·HCl) (CAS: 17193-40-7)
Tlhaloso: Beha hoo e ka bang 2 g ea sampole ka har'a sejana se hloekileng le se omeletseng sa petri 'me u shebe ponahalo ea sampole.Tlaleho: Tlaleha tlhokomeliso
Identificaiton: Bapisa nako ea ho bolokoa ha tlhoro e kholo ea chromatogram ea tokiso ea tlhahlobo le nako ea ho boloka tlhoro e kholo ea chromatogram ea ts'ebetso ea ho lekana ha sistimi joalo ka ha e fumanoa ho chromatographic purity ke HPLC.
Tahlehelo nakong ea ho Omisa: Omisa botlolo e emisitsoeng ka khalase, e sa tebang ea boima ba 105±2℃ metsotso e 30.E lumelle hore e pholile ho mocheso oa kamore ka desciccator.Beha boima ba botlolo e lekanyang ka sethibelo 'me u tlalehe boima ba eona (W1).Fetisetsa ka botlolo e lekanyang boima bo ka bang 1 ho isa ho 2g ka botlolong ea boima.Ka ho sisinya ka bonolo, ka lehlakoreng le leng, abela mohlala oa teko ka ho lekana ka hohle kamoo u ka khonang ho ea botebong ba 5 mm.Beha boima ba botlolo e bekhang 'me u tlalehe boima ba eona (W2).Beha botlolo e laetsoeng ka ontong ea ho omisa, tlosa setopo ebe u se siea hape ka kamoreng.Omisa sampole ea teko ho 105±2℃ bakeng sa lihora tse 3.Ha u bula kamore, koala botlolo hang-hang, 'me ue lumelle hore e fihle mocheso oa kamore ka desiccator.Beha boima ba botlolo ea ho bekha ka ho sebelisa setopo 'me u boele u bokelle boima (W3).Bala tahlehelo ea peresente ha ho omisoa ha sampole ka ho sebelisa foromo e latelang:
Palo Tahlehelo nakong ea ho omisa (% w/w)= (W2-W3) ÷ (W2-W1) x 100
Litaba tsa Enantiomer ka HPLC:
Lik'hemik'hale le Lisebelisoa:
n-Hexane AR kereiti
Methanol HPLC kereiti
Sehlopha sa Isopropyl Alcohol HPLC
Diethylamine AR kereiti
Maemo: L-4-Nitrophenylalanine Methyl Ester HCl e tloaelehileng;L-4-Nitrophenylalanine Methyl Ester HCl enantiomer standard
Mobile Phase: Lokisetsa motsoako oa homogeneous oa n-Hexane, Isopropyl Alcohol, Methanol le Diethylamine (65:20:15:0.1 v/v/v/v)
Boitokisetso bo se nang letho: Fetisetsa 2.5ml ea Methanol ka har'a botlolo ea volumetric ea 10-ml, hlapolla ho ea bophahamong ka karolo ea selefouno ebe u kopanya.
Tokisetso ea ho tšoaneleha ha sistimi:
Beha boima bo nepahetseng 'me u fetisoe ka 5 mg e' ngoe le e 'ngoe ea L-4-Nitrophenylalanine Methyl Ester HCl standard le L-4-Nitrophenylalanine Methyl Ester HCl enantiomer standard ka har'a flask ea volumetric ea 10-ml, qhala hoo e ka bang 2.5ml ea methanol, hlapolla ho bophahamo ba molumo ka selefouno. mokhahlelo le ho kopanya.
Tokisetso ea liteko:
Beha boima ka nepo 'me u fetisetse ka 5 mg ea sampole ka botlolong ea 10-mol volumetric, qhala ka hoo e ka bang 2.5ml ea methanol, hlapolla ho ea bophahamong ka mohato oa selefouno le ho kopanya.
Lisebelisoa tsa lisebelisoa:
Sesebelisoa: HPLC e na le ente, Pump, detector ea UV le Recorder
Kholomo: ChiralPak-IC (4.6mm x 250mm) 5 μ kapa e lekanang le eona
Sekhahla sa phallo: 1.0 ml / motsotso
Wavelength: 260nm
Bophahamo ba ente: 10 μL
Mocheso oa mocheso oa likholomo: 25 ℃
Nako ea ho matha: metsotso e 20
Tsamaiso
Kenya litharollo joalokaha ho boletsoe ka tatellano e latelang.Rekota li-chromatogram 'me u netefatse litekanyetso:
Tlhaloso ea Liente Nomoro ea Mekhoa ea Liente
Boitokisetso bo se nang letho 1 Ha ho tlhoro e sitisang e lokelang ho bonoa ka nako ea ho boloka L-4-Nitrophenylalanine Methyl Ester HCl kapa enantiomer.
Tokisetso ea ho tšoaneleha ha Sistimi 1 Qeto lipakeng tsa L-4-Nitrophenylalanine Methyl Ester HCl le tlhoro ea enantiomer ha ea lokela ho ba ka tlase ho 2.0
Boitokisetso ba liteko 1 Sebelisa chromatogram ea teko ho fumana likahare tsa enantiomer sampole.
Linako Tse Amanang tsa ho Boloka:
L-4-Nitrophenylalanine Methyl Ester HCl 1.0
Enantiomer Mabapi le 1.11
Nako ea ho boloka L-4-Nitrophenylalanine Methyl Ester HCl ke metsotso e ka bang 9.8.
Palo: Ka chromatogram e fumanoeng ho tsoa ho tokiso ea liteko, hlokomoloha litlhōrō tsohle tse lebeletsoeng L-4-Nitrophenylalanine Methyl Ester HCl le Enantiomer.Tlaleha litaba tsa enantiomer sampole ka mokhoa oa ho tloaeleha sebakeng.
Chromatogrpahic Purity le Litšila ka HPLC:
Lik'hemik'hale le Lisebelisoa:
Diammonium phosphate AR kereiti
Phosphoric acid AR kereiti
Methanol HPLC kereiti
Metsi a hloekisitsoeng
Litekanyetso
L-4-Nitrophenylalanine Methyl Ester HCl e tloaelehileng
4-Nitro-L-Phenylalanine monohydrate standard
Mobile Phase-A
Hlakola 6.6 g ya Diammonium phosphate ka 1000ml ea metsi a hloekisitsoeng.Fetola pH ya tharollo ena ho 6.0 ka asiti ya Phosphoric.Sefe le degas.Pele o lekanya pH ya tharollo, lekanya pH mithara ka tharollo e tlwaelehileng ya buffer pH 4.01 LE 7.00.
Mobile Phase-B
Methanol HPLC kereiti
Tokisetso ea ho Lokisoa ha Sisteme
Beha boima bo nepahetseng 'me u fetisoe ka 25 mg e' ngoe le e 'ngoe ea L-4-Nitrophenylalanine Methyl Ester HCl standard le 4-Nitro-L-Phenylalanine monohydrate standard ka 50-ml volumetric flask, qhala ka le ho hlapolla ho bophahamo ba modumo ka methanol le ho kopanya.
Tokisetso ea Teko:
Beha boima bo nepahetseng 'me u fetise ka sampuli ea 25 mg ka har'a botlolo ea volumetric ea 50-ml, qhala ka hare le ho hlapolla ho ea holimo ka methanol le ho kopanya.
Lisebelisoa tsa lisebelisoa:
Sesebelisoa: HPLC e na le ente, Pump, detector ea UV le Recorder
Kholomo: XTerra RP-18 (4.6mm x 150mm) 5 μ kapa e lekanang le eona
Sekhahla sa phallo: 1.0 ml / motsotso
Wavelength: 265nm
Bophahamo ba ente: 20 μL
Mocheso oa mocheso oa likholomo: 25 ℃
Nako ea ho matha: metsotso e 45
Lenaneo la Gradient
Nako (mets) Mobile Phase-A % Mobile Phase-B %
0.01 90 10
15 60 40
35 40 60
40 35 65
41 90 10
45 90 10
Mokhoa:
Kenya litharollo joalokaha ho boletsoe ka tatellano e latelang.Rekota li-chromatogram 'me u netefatse litekanyetso:
Boitokisetso bo se nang letho 1 Ha ho tlhoro e sitisang e lokelang ho bonoa nakong ea ho boloka L-4-Nitrophenylalanine Methyl Ester HCl.
Tokisetso ea ho tšoaneleha ha Sisteme 1 Factor ea mohatla bakeng sa L-4-Nitrophenylalanine Methyl Ester HCl tlhoro ha ea lokela ho ba ka tlase ho 2.0
Boitokisetso ba liteko 1 Sebelisa chromatogram ea teko ho fumana bohloeki ba chromatographic le litšila.
Linako Tse Amanang tsa ho Boloka:
L-4-Nitrophenylalanine Methyl Ester HCl 1.0 (nako ea ho boloka metsotso e ka bang 11.4)
4-Nitro-L-Phenylalanine Ka 0.4
Toluene Ka 2.3
Lipalo
Ka chromatogram e fumanoang ho lokiselitsoeng tlhahlobo, ka mor'a ho lokisoa ho se nang letho, hlokomoloha tlhōrō ka lebaka la toluene.Tlaleha bohloeki ba chromatographic, dikahare tsa 4-Nitro-L-Phenylalanine le litšila tse felletseng tsa sampole ka mokhoa oa tloaelo oa sebaka.