L-Cysteine ​​​​CAS 52-90-4 (H-Cys-OH) Tlhahlobo ea 98.5~101.0% (Titration) Factory ea Boleng bo Phahameng

L-Cysteine ​​​​e na le seng ka tlase ho 98.5% mme e seng ho feta 101.0% ea L-Cysteine ​​​​(C3H7NO2S), e baloang ka mokhoa o omisitsoeng.
Tlhaloso L-Cysteine ​​​​e hlaha e le likristale tse tšoeu kapa phofo e tšoeu ea kristale.E na le monko o ikhethang le tatso e hlabang.E qhibiliha ka bolokolohi metsing, 'me e batla e sa qhibilihe ka ethanol (99.5).E qhibiliha ka 1 mol/L hydrochloric acid TS.
Ho tsebahatsa Lemoha sekhahla sa ho monya ha infrared sa L-Cysteine ​​joalokaha se laetsoe ka mokhoa oa potassium bromide disk tlas'a Infrared Spectrophotometry <2.25>, 'me u bapise spectrum le Reference Spectrum: ka bobeli li bonts'a matla a tšoanang a ho monya ka linomoro tse tšoanang tsa maqhubu.
Optical rotation <2.49> [a]20D:+8.0 ~+10.0° (2 g e baloang ka motheo o omisitsoeng, 1 mol / L hydrochloric acid TS, 25mL, 100 mm).
pH <2.54> PH ea tharollo e lokiselitsoeng ka ho qhibiliha 1.25 g ea L-Cysteine ​​​​ka 50 mL ea metsi ke 4.7 ho 5.7.
Bohloeki (1) Ho hlaka le 'mala oa tharollo-Hlakola 1.0 g ea L-Cysteine ​​​​ka 20 mL ea metsi: tharollo e hlakile ebile ha e na mebala.
(2) Chloride <1.03>-Hlakola 0.30 g ya L-Cysteine ​​​​ka 10mL ea diluted nitric acid (1 ho 4), eketsa 10mL ea hydrogenperoxide (30), futhumatsa metsotso e 20 ka bateng ea metsi a belang, a pholile, ebe u eketsa metsi ho etsa 50 ml.Etsa tlhahlobo ka ho sebelisa tharollo ena e le tharollo ea teko.Lokisetsa tharollo ea taolo ka 0.35 mL ea 0.01 mol/L hydrochloric acid VS (e seng ho feta 0.041%).
(3) Sulfate <1.14>-Hlakola 0.6 g ea L-Cysteine ​​​​ka metsi a 30 ml le 3 ml ea dilute hydrochloric acid, 'me u kenye metsi ho etsa 50 ml.Etsa tlhahlobo ka ho sebelisa tharollo ena e le tharollo ea teko.Lokisetsa tharollo ea taolo ka tsela e latelang: Ho 0.35 mL ea 0.005 mol/L sulfuric acid VS eketsa 3 mL ea dilute hydrochloric acid le metsi ho etsa 50 mL.Lokisetsa tharollo ea teko le tharollo ea taolo ka 4 mL ea barium chloride TS, ka ho latellana (e seng ho feta 0.028%).
(4) Ammonium <1.02> -Etsa teko ka 0.25 g ea L-Cysteine ​​​​, ho sebelisa distillation tlas'a khatello e fokotsehileng.Lokisetsa tharollo ea taolo ka 5.0 mL ea Standard Ammonium Solution (e seng ho feta 0.02%).
(5) Heavy Metals <1.07>-Tsoela pele ka 1.0g ea L-Cysteine ​​​​ho ea ka Method 4, 'me u etse tlhahlobo.Lokisetsa tharollo ea taolo ka 1.0 mL ea Standard Lead Solution (eseng ho feta 10 ppm).
(6) Iron <1.10> -Lokisetsa tharollo ea teko ka 1.0 g ea L-Cysteine ​​​​ho ea ka Mokhoa oa 1, 'me u etse tlhahlobo ho ea ka Mokhoa oa A. Lokisetsa tharollo ea taolo ka 1.0mL ea Standard Iron Solution (eseng ho feta 10 ppm ).
(7)Lintho Tse Amanang le Tsona-Hlakola 0.10 g ea L-Cysteine ​​ka tharollo ea N-ethylmaleimide (1 ho 50) ho etsa 10 mL hantle, tlohela metsotso e 30, 'me u sebelise tharollo ena e le mohlala oa tharollo.Pipete 1 ml ea tharollo ea sampole, eketsa metsi ho etsa hantle 10 ml, pipet 1 ml ea tharollo ena, eketsa metsi ho etsa 50 ml hantle, 'me u sebelise tharollo ena e le tharollo e tloaelehileng (1).Ka thoko, qhala 0.10 g ya L-cystine ho 0.5mol/L hydrochloric acid TS ho etsa 20 ml hantle.Pipete 1mL ea tharollo ena, tšela metsi ho etsa 100 ml, 'me u sebelise tharollo ena e le tharollo e tloaelehileng (2).Etsa tlhahlobo ka litharollo tsena joalokaha ho laetsoe tlas'a Thin-layer Chromatography <2.03>.Spot 10mL e 'ngoe le e 'ngoe ea sampole ea tharollo le tharollo e tloaelehileng (1) le (2) poleiting ea silika ea silika bakeng sa chromatography ea lera le tšesaane.Theha poleiti ka motsoako oa1-butanol, metsi, le acetic acid (100) (3:1:1) ho ea bohōle ba 10 cm, 'me u omise poleiti ka metsotso e 30 ka 809C.Fafatsa poleiti ka ho lekana ka tharollo. ea ninhydrin ka motsoako oa methanol le acetic acid (100) (97: 3) (1 ho 100), ebe mocheso ho 80 ℃ metsotso e 10: sebaka se fumanoeng ho tsoa ho sampuli ea tharollo e lumellanang le sebaka se fumanoang ho tsoa tharollong e tloaelehileng. (2) ha e matla ho feta sebaka se tsoang ho tharollo e tloaelehileng (2).Hape, matheba ntle le sebaka se ka sehloohong le matheba a boletsoeng ka holimo ho tsoa mohloling oa tharollo ha a matla ho feta sebaka sa tharollo e tloaelehileng (1).
Tahlehelo ha o Omisa <2.41> Ha e fete 0.50% (1 g, ka vacuum, phosphorus (V) oxide, lihora tse 3).
Masalla a ho Ignition <2.44> Ha a fete 0.1%(1 g).
Assay Beha boima ba 'mele bo nepahetseng ka 0.2 g ea L-Cysteine, e behe ka botlolo e emisitsoeng, 'me u qhale ka 20 ml ea metsi.Qhala 4 g ea iodide ea potassium ka tharollo ena, hang-hang u e behe ka metsing a leqhoa, eketsa 5 mL ea dilute hydrochloric acid le hantle 25 ml ea 0.05 mol / L ea iodine VS, tlohela sebakeng se lefifi ka metsotso e 20, ebe u titrate<2.50 > bongata bo feteletseng ba iodine ka 0.1 mol/L sodium thiosulfate VS (sesupo: starch TS).Etsa qeto e se nang letho u sebelisa mokhoa o tšoanang.
ML ka 'ngoe ea 0.05 mol/L iodine VS＝12.12 mg ea C3H7NO2S
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