L-Glutamine CAS 56-85-9 (H-Gln-OH) Tlhahlobo ea 99.0~101.0% Factory High Quality

Pharmacopoeia ea JapaneReference Standards
L-Glutamine [56-85-9]
L-Glutamine, ha e omisitsoe, ha e na ka tlase ho 99.0% le ho feta 101.0% ea L-Glutamine (C5H10N2O3).
Tlhaloso L-Glutamine e hlaha e le tšoeu, likristale kapa phofo ea crystalline.E na le tatso e nyane ea sebopeho
E qhibiliha ka bolokolohi ka formic acid, e qhibiliha ka metsing, 'me e batla e sa qhibilihe ka ethanol (99.5)
Identication Hlalosa hore na infrared absorption spectrum ea L-Glutamine e laetsoe ka mokhoa oa potassium bromide disk tlas'a Infrared Spectrophotometry <2.25>, 'me u bapise spectrum le Reference Spectrum: ka bobeli li bonts'a matla a tšoanang a ho monya ka linomoro tse tšoanang tsa maqhubu.
Optical Rotation <2.49> [a]20D:+6.3° to +7.3° Beha boima bo nepahetseng ka 2 g ya L-Glutamine, e omisitsweng pele, eketsa 45mL ya metsi, e futhumala ho 40℃ ho qhala, mme ka mora ho tsidifala, eketsa metsi ho etsa. hantle 50 ml.Etsa qeto ea ho potoloha ha optical ea tharollo ena ka seleng ea 100-mm, ka hare ho metsotso e 60.
pH <2.54> PH ea tharollo e lokiselitsoeng ka ho qhibiliha 1.0 g ea L-Glutamine ka 50 mL ea metsi e pakeng tsa 4.5 le 6.0.
Bohloeki
(1) Ho hlaka le 'mala oa tharollo-Tharollo e fumanoang ka ho qhibiliha 0.5 g ea L-Glutamine ka 20 ml ea metsi e hlakile ebile ha e na mebala.
(2) Chloride <1.03> -Etsa tlhahlobo ka 0.5 g ea L-Glutamine.Lokisetsa tharollo ea taolo ka 0.30 mL ea 0.01 mol/L hydrochloric acid VS (e seng ho feta 0.021%).
(3) Sulfate <1.14> -Etsa tlhahlobo ka 0.6 g ea L-Glutamine.Lokisetsa tharollo ea taolo ka 0.35 mL ea 0.005 mol/L sulfuric acid VS (eseng ho feta 0.028z).
(4) Ammonium <1.02> -Etsa tlhahlobo ka 0.10 g ea L-Glutamine, u sebelisa distillation tlas'a khatello e fokotsehileng.Lokisetsa tharollo ea taolo ka 10.0 mL ea Standard Ammonium Solution.Mocheso oa ho hlapa metsi ke 45 ℃ (eseng ho feta 0.1%).
(5) Heavy Metals <1.07> - Tsoela pele ka 1.0 g ea L-Glutamine ho ea ka Mokhoa oa 1, 'me u etse tlhahlobo.Lokisetsa tharollo ea taolo ka 1.0 mL ea Standard Lead Solution (eseng ho feta 10 ppm).
(6) Iron <1.10> - Lokisetsa tharollo ea teko ka 1.0 g ea L-Glutamine ho ea ka Method 1, 'me u etse tlhahlobo ho ea ka Mokhoa oa A. Lokisetsa tharollo ea taolo ka 1.0 mL ea Standard Iron Solution (e seng ho feta 10 ppm)
(7) Lintho Tse Amanang - Hlakola 0.10 g ea L-Glutaminein 10 mL ea metsi, 'me u sebelise tharollo ena e le mohlala oa tharollo.Pipete 1 ml ea tharollo ea sampole, eketsa metsi ho etsa 10 ml hantle.Pipet 1 mL ea tharollo ena, eketsa metsi ho etsa 50 ml hantle, 'me u sebelise tharollo ena e le tharollo e tloaelehileng.Etsa tlhahlobo ka litharollo tsena joalokaha ho laetsoe tlas'a Thin-layer Chromatography <2.03>.Spot 5mL e 'ngoe le e 'ngoe ea tharollo ea sampole le tharollo e tloaelehileng poleiting ea silicagel bakeng sa chromatography ea lera le tšesaane.Ebe u ntshetsa pele ka motsoako oa 1-butanol, metsi le acetic acid (100) (3:1:1) ho ea bohōle ba 10 cm, 'me u omise poleiti ka 80 ℃ metsotso e 30.Fafatsa ka ho lekana tharollo ea ninhydrin ka motsoako oa methanol le acetic acid (100) (97:3) (1 ho 100) poleiting, 'me mocheso ka 80 ℃ metsotso e 10: sebaka se seng ntle le sebaka se ka sehloohong se fumanoeng ka tharollo ea sampole ha e matla ho feta sebaka se fumanoeng ka tharollo e tloaelehileng.
Tahlehelo ha o Omisa <2.41> Ha e fete 0.3% (1 g, 105℃, lihora tse 3)
Masalla a ho Ignition <2.44> Ha a fete 0.10%(1 g).
Assay Beha boima bo nepahetseng ka 0.15 g ea L-Glutamine, e neng e omisitsoe pele, qhala ka 3 mL ea formic acid, eketsa 50 mL ea acetic acid (100), le titrate <2.50> ka 0.1 mol/L perchloric acid VS (potentiometric titration).Etsa qeto e se nang letho ka mokhoa o ts'oanang, 'me u etse tokiso e hlokahalang.
ML ka 'ngoe ea 0.1 mol/L perchloric acid VS＝14.61 mg ea C5H10N2O3
Lijana le Lijana tsa Polokelo—Lijana tse tiileng.

USP Reference Standards <11> -USP Glutamine RS.
Boitsebiso, Infrared Absorption <197K>.
Potoloho e khethehileng <781S>: pakeng tsa +6.3 ° le +7.3 °, e ikemiselitse ho 20℃
Tharollo ea Teko- Ka botlolong e loketseng, lokisa ka nepo tharollo ea hoo e ka bang 40mg ka ml ka metsing hoo e ka bang 40 ℃.Pholisa 'me u hlapolle ka metsi ho ea holimo pele o sebelisoa.
Tahlehelo ka ho Omisa <731>-Omisa ho 105 ℃ bakeng sa lihora tse 3;ha e lahleheloe ke ho feta 0.20% ea boima ba eona.
Masalla a ho Ignition <281>Ha e fete 0.30%
Chloride <221>-Karolo ea 0.7-g ha e bontše chloride ho feta e lumellanang le 0.50ml ea 0.020 N hydrochloric acid: ha e fumanehe ho feta 0.05%.
Sulfate <221>-Karolo ea 0.8-g ha e bontše sulfate ho feta e lumellanang le 0.25ml ea 0.020 N sulfuric acid: ha e fumanehe ho feta 0.03%.
Bohloeki ba Chromatographic
Adsorbent: 0.25-mm lera la chromatographic silica gel motsoako.
Tharollo ea Teko: 10mg ka ml, ka metsing
Tharollo e Tloaelehileng-Lokisetsa tharollo ea USP Glutamine RS metsing a nang le motsoako o tsejoang oa hoo e ka bang 0.05mg ka ml.
Bophahamo ba Kopo: 5μL
Ho Ntlafatsa Setsi sa Solvent: motsoako oa joala ba butyl, metsi, le glacial acetic acid (3:1:1).
Spray Reagent-Dissolve 0.2g ya ninhydrin ka 100ml ya motswako wa butyl alcohol le 2 N acetic acid (95:5)
Ts'ebetso-Tsoela pele joalo ka ha o laetsoe bakeng sa chromatography ea lera le tšesaane tlasa Chromatography <621>, ebe o omisa poleiti ho 80 ℃ metsotso e 30.Fafatsa poleiti ka sesebelisoa sa ho fafatsa, futhumatsa ka 80 ℃ metsotso e 10, 'me u hlahlobe ka tlas'a lebone le lesoeu: ha ho sebaka sa bobeli ho chromatogram se fumanoeng ho tsoa tharollong ea teko se seholo kapa se matla ho feta sebaka sa mantlha sa chromatogram se fumanoeng ho tsoa tharollong ea standrad. (0.5%)
Litšila tsa Organic Volatile, Mokhoa oa I <467>: Kopana le litlhoko.
Assy-Fetisetsa hoo e ka bang 150mg ea Glutamine, e lekantsoeng hantle, ho 125ml flask, qhala ka 3ml ea formic acid le 50ml ea glacial acetic acid, le titrate e nang le 0.1 N perchloric acid VS e khethollang qetello ea potentiometrically.Etsa qeto e se nang letho, 'me u etse tokiso e hlokahalang (sheba Titrimetry<541>).ml ka 'ngoe ea 0.1 N perchloric acid e lekana le 14.615 mg ea C5H10N2O3.