L-Leucine Methyl Ester Hydrochloride CAS 7517-19-3 (H-Leu-OMe·HCl) Assay >99.0% Factory

1. Ponahalo
Nka palo e loketseng ea sehlahisoa sena, e behe pampiring e tšoeu e boreleli 'me u hasane 5 cm2 le 5 mm thick.E shebelle sebakeng se khanyang.
2. Purity (HPLC)
2.1 Seletsa
chromatography ea mokelikeli e phahameng ea ts'ebetso, mochini oa PDA.
Elektronike analytical balance
2.2 Li-reagents
Acetonitrile (mophato wa chromatographic), TFA(grade chromatographic)
2.3 Maemo a Chromatographic
2.3.1 Kholomo: YMC-ODS-AM, 5 μl, 150 x 4.6 mm
2.3.2
Leqhubu la ho lemoha: INC220nm
Sekhahla sa phallo: 1.0mL/min
Bophahamo ba ente: 10 μL (bakeng sa litšupiso)
Monyetla: Acetonitrile
Nako ea ho matha: metsotso e 20
2.4 Ho lokisetsa mohato oa Mobile
Mobile phase A (0.1% trifluoroacetic acid metsi): ho monya ka nepo 2.0ml TFA, Hlakola ho 2000ml ka metsi, kopanya le degas;
Mobile phase B (0.1% trifluoroacetic acid acetonitrile): ho monya ka nepo 2.0ml TFA, Fokotsa ho 2000ml ka acetonitrile, motsoako le degas;
2.5 Mekhahlelo ea liletsa
Nako (mets) A% B%
0.00 90 10
12.00 10 90
15.00 10 90
15.01 90 10
20.00 90 10
2.6 Tokiso ea tharollo ea mohlala
Beha le ho qhala sampole ea 0.1g ka acetonitrile, hlapolla ho 100ml, sisinya hantle bakeng sa tšebeliso, kapa mahloriso a tšoanang.Lokisetsa disampole tse pedi ka ho bapana.
2.7 Boikemisetso ba Mohlala
Hlakola mohlala ho latela mokhoa o latelang oa lisampole:
Ho feta ente e le 'ngoe ea tharollo e se nang letho
1 ea mohlala oa nale tharollo 1#
1 tharollo ea nale 2#
2.8 Sephetho Palo
2.8.1 Mokhoa oa ho tloaeleha ha sebaka sa tlhoro o ile oa sebelisoa ho bala bohloeki ba HPLC ka ho tlosa sebaka se se nang letho.
2.8.2 Phapang e lekanyelitsoeng ea bohloeki ba linale tse peli ha ea lokela ho feta 1%
2.8.3 Haeba liphello tsa liente ka bobeli li finyella litekanyetso tsa ho amoheloa, bohloeki bo tloaelehileng bo nkoa e le sephetho sa ho qetela.
3, Ntho e qhibilihang -- RY-1 sesebelisoa sa ntlha ea ho qhibiliha
4. Tahlehelo ka mokhoa oa ho omisa teko
4.1 Lisebelisoa:
Ovine ea ho omisa ea thermostatic ea motlakase, tekanyo ea 1/10,000.
4.2 Mokhoa:
Ka botlolong ea boima bo bataletseng e nang le boima bo sa khaotseng le sekoahelo sa molomo oa fatše o ommeng ho feta tekano, bekha gram e le 1 (e nepahetseng ho isa ho 0.0001 gram) ea sampole.Sampole e lokela ho aloa ka mokhoa o ts'oanang ka tlase ho botlolo e lekanyang ka botenya bo sa feteng 10mm, e kenngoe ka ontong ea ho omisa ea thermostatic, e omelle ka 105 ~ 110 ℃ ka lihora tse 3, ebe o kena ka phapusing ea ho omisa ho pholile. mocheso oa kamore bakeng sa ho bekha.
Palo: Tahlehelo ha ho omisoa %= (M1-M2) ÷M×100
Moo: M1: boima ba sampole le botlolo ea ho metha pele e omisoa, ligrama
M2: boima ba sampuli le botlolo ea ho lekanya ka mor'a ho omisa, ka ligrama
M: Boima ba mohlala, ligrama
5. Ho potoloha ho khethehileng
- Potoloho e khethehileng e lekantsoe ho latela GB/T613-1988
Boitokisetso ba mohlala: 1.0000g sampole e ne e lekantsoe ka nepo 'me ea isoa botlolong e hloekileng le e omileng ea 50ml, metsi a 20ml a ile a eketsoa, ​​​​botlolo e ile ea koaheloa le ho sisinyeha hore e qhibilihe, ebe e hlapolloa ka sekala ka metsi.
Teko: Fetola zero ea gyroscope pele ho tlhahlobo, ebe u kenya tube ea teko ka tharollo ea sampole, rekota Angle ea ho potoloha, 'me u bale ho potoloha ho itseng ha sampole ka foromo e latelang.
[а]D20 = (r×50) ÷ (L×W)
[а]D20: Potoloho e khethehileng ea optical ho 20 ℃ ea tharollo ea sampole
r: Potoloho e bonoang ho 20 ℃ bakeng sa tharollo ea sampole
50: Bolumo ea tharollo ea sampole e lokisitsoeng (ml)
w: Boima ba mohlala (g)
L: Bolelele ba tube ea optical (dm)