L-(-)-Tyrosine CAS 60-18-4 (H-Tyr-OH) Assay 98.5~101.5% Factory High Quality

L-(-)-Tyrosine (H-Tyr-OH) (CAS: 60-18-4) AJI 97 Mokhoa oa Teko
Identification: Bapisa spectrum ea infrared absorption ea sampole le ea standard by potassium bromide disc method.
Potoloho e Khethehileng [α]20/D: Mohlala o omisitsoeng, C=5, 1mol/L HCl
Boemo ba Tharollo (Transmittance): 0.5g ho 10ml ea 1mol / L HCl, spectrophotometer, 430nm, 10mm lisele tse botenya.
Chloride (Cl): 0.7g, A-1, ref: 0.40ml ea 0.01mol/L HCl
Ammonium (NH4): B-2
Sulfate (SO4): 1.2g, (1), ref: 0.50ml ea 0.005mol/L H2SO4
Tšepe (Fe): 1.5g, (2), ref: 1.5ml ea Iron Std.(0.01mg/ml)
Metals e boima (Pb): 2.0g, (2), ref: 2.0ml ea Pb Std.(0.01mg/ml)
Arsenic (As2O3): 2.0g, (2), ref: 2.0ml ea As2O3 Std.
Li-Amino Acids tse ling: Hlakola ammonia R2 Mohlala oa Teko: 50μg, F-6-a, taolo: L-Tyr 0.25μg
Tahlehelo ha o Omisa: ho 105 ℃ bakeng sa lihora tse 3.
Residue on Ignition (Sulfated): AJI Test 13
Assay: Sampole e omisitsoeng, 180mg, (1), 6ml ea formic acid, 50ml ea glacial acetic acid, 0.1mol/L HCLO4 1ml = 18.119mg C9H11NO3
Teko ea pH: 0.02g ho 100ml e tletseng metsi a metsi

L-(-)-Tyrosine (H-Tyr-OH) (CAS: 60-18-4) USP35 Mokhoa oa Teko
TLHALOSO
Tyrosine e na le NLT 98.5% le NMT 101.5% ea L-Tyrosine (C9H11NO3), e baloang ka mokhoa o omisitsoeng.
TS'ELISO TS'OANE
A. HO AMOHELA INFORARED <197K>
TLHOKOMELO
MOKHOAOA
Mohlala: 180 mg ea Tyrosine
Sekheo: Kopanya 6 mL ea formic acid le 50 ml ea glacial acetic acid.
Sistimi ea Titrimetric
(Sheba Titrimetry <541>)
Mokhoa: Titration e tobileng
Titrant: 0.1 N perchloric acid VS
Ho lemoha lintlha tsa ho qetela: Potentiometric
Tlhahlobo: Qhala Mohlala ho 6 mL ea formic acid le 50mL ea glacial acetic acid, le titrate le Titrant.Etsa boitlamo bo se nang letho.
Bala liperesente tsa tyrosine (C9H11NO3) ho Mohlala o nkiloeng:
Sephetho = {[(VS-VB) x N x F]/W} x100
VS= bophahamo ba modumo ea Titrant e jeoang ke Mohlala (mL)
VB= bophahamo ba Titrant bo jeoang ke Blank (mL)
N= tloaelo ea 'nete ea Titrant (mEq/mL)
F= ntlha e lekanang, 181.2 mg/mEq
W= Boima ba mohlala (mg)
Mekhoa ea ho amohela: 98.5% ~ 101.5% ka mokhoa o omisitsoeng
LITŠILA
HO HLAHALA HO HLAHALA <281>: NMT 0.4%
CHLORIDE LE SULFATE, Chloride <221>
Tharollo e tloaelehileng: 0.20mL ea 0.020 N hydrochloric acid
Mohlala: 0,35 g ea Tyrosine.[ HLOKOMELA-Haeba ho hlokahala, qhala Mohlala ka ho futhumatsa hoo e batlang e bela le ho eketsa 1 mL ea nitric acid.]
Maemo a kamohelo: NMT 0.04%
CHLORIDE LE SULFATE, Sulfate <221>
Tharollo e tloaelehileng: 0.50mL ea 0.020 N sulfuric acid
Mohlala: 1.2 g ea Tyrosine.[HLOKOMELA-Haeba ho hlokahala, qhala Mohlala ka ho eketsa 6mL ea diluted hydrochloric acid.]
Maemo a kamohelo: NMT 0.04%
IRON <241>: NMT 30ppm [HLOKOMELA-Ha ho hlokahala, sebelisa 2 mL ea hydrochloric acid ho qhala Mohlala.]
LINTLHA TSE BOHLOKO, Mokhoa oa I <231>: NMT 15ppm
TS'OANELO MAKANYANA
Tharollo ea ammonia e hlapolotsoeng: Hlakola 16mL ea ammonia e tsitsitseng ka metsi ho ea ho 100 ml.
Tharollo ea ho tšoaneleha tsamaisong: Qhala 10 mg ea USP L-Tyrosine RS le 10 mg ea USP L-Phenylalanine RS ka 1 mL ea Diluted ammonia solution, 'me u hlapolle ka metsi ho 25.0 mL.
Tharollo e tloaelehileng: Ka botlolong e loketseng, qhala palo ea USP L-Tyrosine RS ka 1 mL ea Diluted ammonia solution.Hlakola ka metsi ho ea bophahamong ho fumana tharollo ea 0.05mg/mL.[HLOKOMELA-Tharollo ena e na le mahloriso a lekanang le 0.5% ea tharollo ea Mohlala.]
Tharollo ea mohlala: Fetisetsa 0.1 g ea Tyrosine ka har'a botlolo ea volumetric ea 10-mL, eketsa tharollo e lekaneng ea Diluted ammonia ho qhibiliha, 'me u hlapolle ka metsi ho ea bophahamong.
Sistimi ea Chromatographic (Sheba Chromatography <621>, Thin-Layer Chromatography.)
Mokhoa: TLC
Adsorbent: 0.25-mm lera la chromatographic silica gel motsoako
Bophahamo ba kopo: 5μL
Ho ntshetsa pele tsamaiso ya solvent: Isopropyl alcohol le ammonium hydroxide (7:3)
Spray reagent: 2 mg/mL ya ninhydrin motswakong wa butyl alcohol le 2N acetic acid (95:5)
Ho nepahala ha sistimi
Litlhoko tsa ho tšoaneleha: Chromatogram ea tharollo ea ts'ebetso ea Sistimi e bonts'a matheba a mabeli a arohaneng ka ho hlaka.
Tlhahlobo
Mehlala: Tharollo ea ho tšoaneleha ha Sisteme, Tharollo e tloaelehileng, le Tharollo ea Mohlala.
Omisa poleiti pakeng tsa 100 ° le 105 ° ho fihlela ammonia e nyamela ka ho feletseng.Fafatsa ka sesebelisoa sa Spray, 'me u chese pakeng tsa 100 ° le 105 ° metsotso e 15.Hlahloba poleiti tlas'a khanya e tšoeu.
Mokhoa oa ho amohela: Sebaka sefe kapa sefe sa bobeli sa tharollo ea Sample ha se seholo kapa se matla ho feta sebaka sa mantlha sa tharollo ea Maemo.
Tšilafalo efe kapa efe ea Motho: NMT 0.5%
Kakaretso ea litšila: NMT 2.0%
LITEKO TSEKO
PHETOHO EA MAIKUTLO, Phapanyetsano e Khethehileng <781S>
Tharollo ea mohlala: 50 mg/mL ho 1 N hydrochloric acid
Mekhoa ea ho amohela: -9.8° ho ea ho -11.2°
LOSSON KA HO OMETSA <731>: Omisa sampole ho 105℃ bakeng sa 3 h: e lahleheloa ke NMT 0.3% ea boima ba eona.
KEKELETSO LIHLOKO
HO PAKERA LE HO BOLOKA: Boloka ka har'a linkho tse koetsoeng hantle.
USP REFERENCE LITEKELO <11>
USP L-Phenylalanine RS
USP L-Tyrosine RS