Thepa: Sesebelisoa sa GC (Shimadzu GC-2010)
Kholomo: DB-17 Agilent 30mX0.53mmX1.0μm
Mocheso oa pele oa ontong: 80 ℃
Nako ea pele 2.0min
Reiti 15℃/min
Mocheso oa ho qetela oa ontong: 250 ℃
Nako ea ho qetela 20min
Khase e tsamaisang Nitrogen
Mokhoa oa ho phalla kamehla
Phallo 5.0mL/min
Arohane karo-karolelano ea 10: 1
Mocheso oa ente: 250 ℃
Mocheso oa detector: 300 ℃
Bophahamo ba ente 1.0μL
Mehato e lokelang ho nkuoa pele ho tlhahlobo:
1. Kholomo ea boemo ho 240℃ bakeng sa bonyane metsotso e 30.
2. Hlatsoa syringe 'me u hloekise liner ea ente hantle ho tlosa litšila tsa tlhahlobo ea pejana.
3. Hlatsoa, o omise, 'me u tlatse metsi ka har'a linkho tsa ho hlatsoa seringe.
Boitokisetso ba diluent:
Lokisetsa tharollo ea 2% w / v sodium hydroxide ka metsing.
Tokisetso e tloaelehileng:
Beha boima ba hoo e ka bang 100mg ya (R) -3-hydroxyprrolidine hydrochloride standard ho ba vial, eketsa 1mL ya diluent mme o qhale.
Tokisetso ea liteko:
Beha boima ba 100mg ea sampole ea tlhahlobo ka har'a vial, eketsa 1mL ea diluent mme o qhale.Lokisetsa ka koporo.
Mokhoa:
Ente e se nang letho (diluent), boitokiso bo tloaelehileng le boitokisetso ba liteko u sebelisa maemo a kaholimo ho GC.Hlokomoloha litlhōrō ka lebaka la ho hloka letho.Nako ea ho boloka tlhōrō ka lebaka la (R) -3-hydroxyprrolidine e ka bang 5.0min.
Hlokomela:
Tlaleha sephetho joalo ka karolelano
Nako ea poso: Nov-13-2021