Phthalimide Potassium Salt CAS 1074-82-4 Bohloeki >99.0% (HPLC)

3. Mokhoa oa teko
3.1 Tahlehelo nakong ea ho Omisa (W/W)
3.1.1 Thepa le lisebelisoa
E 'ngoe e fetang likete tse leshome tsa tekanyo ea elektroniki, ontong ea ho omisa ea thermostatic, botlolo ea boima ba 40 * 25, se omisang.
Reagent: gel ea silika
3.1.2 Mekhoa
1. Hloekisa libotlolo tse bekhang tse 3 40*25.
2, kenya ka ontong ka 105 ℃ (± 2 ℃) e omme bakeng sa hora e le 'ngoe.
3. Ntša botlolo ea boima ba 40 * 25 'me ue kenye ka har'a se omisang ho pholile bakeng sa 30min.
4, Boima bo nepahetseng ba 40 * 25 botlolo ea ho lekanya e se nang letho W0.
5. Sebelisa botlolo ea ho bekha ea 40 * 25 ho lekanya sampole e tsoakiloeng ka ho lekana ea 3.0000g (W1), kakaretso ea 3.
6, Kenya ka ontong, tlosa sekoaelo sa botlolo, se behe pela botlolo ea boima, kapa halofo bula sekoaelo sa botlolo hore se omisoe, se ntše, se be se bekhang hantle sa botlolo, se omelle ho 105℃ (±2℃) bakeng sa 3 lihora.
7. Ntša botlolo ea boima ba 40 * 25 'me ue kenye ka har'a sesebelisoa sa ho omisa ho pholile bakeng sa 30min.
8, Boima bo nepahetseng ka mor'a ho omisa boima ba 40 * 25 ba botlolo ea W2.
Mokhoa oa ho bala: Tahlehelo ha o Omisa (%) =(W1-W2)/(W1-W0)×100%
3. 4 Boikemisetso ba litaba
3.4.1 Lisebelisoa le lisebelisoa
LC-UV100 Liquid Chromatograph (Shanghai Wufeng Scientific Instrument Co., LTD.)
10ml botlolo ea volumetric, khaba ea sekhutlo ea tšepe e sa hloekang le lisebelisoa tse ling tse amanang
3.3.2 Mekhoa
Mokhoa oa ho hlahloba letsoai la Phthalimide Potassium
3.3.3 Chromatography ea metsi
Boemo ba chromatographic
Kholomo ea Chromatographic: HypersilC6H5 (C8 e fumaneha hape) 4.6*250mm 5um
Leqhubu la ho lemoha: 210nm
Mocheso oa kholomo: 30 ℃
Sekhahla sa phallo: 1.0ml/min
Sample concentration: 0.05mg/ml (diluent ke mobile phase)
Li-reagents
Potassium Phosphate Dibasic (analytical pure)
Letsoai la Potassium ea Phthalimide (sampuli e tloaelehileng)
Acetonitrile (analytical pure)
Metsi a hloekileng
Mokhahlelo oa selefouno: acetonitrile: phosphate buffer (PH:3.5) =40:60
Beha 1.63g Potassium Phosphate Dibasic 'me u qhale ka 600ml metsi a hloekileng, fetola boleng ba PH ho 3.5 ka phosphoric acid, tšela ka 1000ml beaker e nang le 400ml acetonitrile, kopanya hantle, sefa se nang le 0.45um e tsoakiloeng ka fiber le membrane ea ultrasonic, e qhibilihisa kopo.
3.3.4 Tsamaiso
1) Tharollo ea tlhahlobo ea tlhophiso (0.05mg/ml)
Mohlala oa 2.5mg o ile oa lekanngoa ka nepo 'me oa kenngoa ka botlolo ea 50ml ea volumetric.Mokhahlelo oa selefouno o ile oa eketsoa mme oa hlapolloa ho isa tekanyong ka ho qhibiliha ha ultrasonic.
2) Tharollo e tloaelehileng ea tlhophiso (1.0mg/ml)
Mohlala o tloaelehileng oa 2.5mg o ile oa lekanngoa ka nepo 'me oa kenngoa ka har'a botlolo ea volumetric ea 50ml.Mokhahlelo oa selefouno o ile oa eketsoa mme oa hlapolloa ho isa tekanyong ka ho qhibiliha ha ultrasonic.
3) Ts'ebeliso ea sistimi:
(1) Beha hlooho ea filthara ka 80% methanol ha u qala, bula valve ea ho ntša metsi 'me u tšollele 3-5min,' me u koale valve ea ho qeta le ho hlatsoa 10-15min.Ebe u kenya hlooho ea filthara ka har'a metsi a hloekileng a 90%, bula valve ea drain 'me u tšollele 3-5min, koala valve ea drain ebe u hlatsoa 10min.
(2) Ebe u fetola karolo ea selefouno e lokiselitsoeng, sekhahla sa phallo ke 1.0ml / min, khatello ea pele ea kholomo e ka tlase ho 1000Psi, bula sesebelisoa, emela ho fihlela khatello e ka pele ea kholomo e tsitsitse, bula sebaka sa ts'ebetso sa komporo, 'me u kene inthaneteng.
(3) Kamora hore khatello e ka pele ea kholomo e tsitsitse, bula sesebelisoa.Enta nale e le 1 ea mokhahlelo oa selefouno, ebe o kenya nale e le 1 ea sampole e tloaelehileng, 'me qetellong u ente sampole.
(4) Liphetho tsa lipalo: mokhoa oa ho tloaeleha sebakeng
(5) Ka mor'a ho qeta mosebetsi oa ho hlahloba, koala detector pele, ebe o hlatsoa hlooho ea filthara metsing a hloekileng bakeng sa 10-15min;Ka nako e ts'oanang, valve ea ente e ne e hlatsuoa ka metsi le methanol.
(6) Qetellong, apara 80% methanol, hlatsoa bakeng sa 30min, 'me u koale.
4. Ka mor'a ho phethoa ha tlhahlobo, tlatsa pampiri ea tlhahlobo le ea laboratori.