I-Adenosine CAS 58-61-7 Assay 99.0% -101.0% USP Standard Factory High Purity

INGCACISO
I-Adenosine iqulethe i-NLT 99.0% kunye ne-NMT 101.0% ye-C10H13N5O4, ibalwe ngokomisiweyo.
UKUCHAZA
• A. UFUMBO LWE-IFRARED <197K>: NMT 0.1%
UVAVANYO
• I-ADENOSINE
Isampuli: 200 mg ye-Adenosine ngaphambili yomiswe kwi-105 ° ye-2h
Inkqubo yeTitrimetric
(Jonga i-Titrimetry <541>)
Imowudi: Ukubhalwa ngokuthe ngqo
I-Titrant: 0.1 N nge-asidi ye-chloric VS
Ukubonwa kwendawo yokugqibela: I-Potentiometric
Uhlalutyo: Dissolve kwi-50 mL ye-glacial acetic acid kunye ne-titrate nge-0.1 N nge-chloric acid VS.Bala ipesenti yeAdenosine (C10H13N5O4) kwisahlulo esithathiweyo:
Isiphumo = [(V − B) × N × F × 100]/W
V = Isampulu yevolumu yetitrant (mL)
B = Umthamo wetitranti engenanto (mL)
N= ukuqheleka okuthambileyo (mEq/mL)
F= into elinganayo: 267.25 mg/mEq
W= ubunzima beSampuli (mg)
Indlela yokwamkela: 99.0% -101.0% kwisiseko esomileyo
• UBUNGCOLA
• UKUBULA OKUSELELEYO <281>: NMT 0.1%
• IIMETALI EZISINZI, Indlela II <231>: NMT 10 ppm
• UMDA WE-AMMONIA
Isisombululo sesampuli: Nxiba i-0.5 g kwi-10 mL yamanzi.Gxuma i-30 s, kwaye udlule kwisihluzo esirhabaxa.Nciphisa i-filtrate ngamanzi kwi-15 mL, kwaye usebenzise i-filtrate.
Isisombululo esisemgangathweni: 0.4 µg/mL yeammonium chloride emanzini
Uhlalutyo: Kwisisombululo seSampuli kunye nesisombululo esiPhakathi songeza i-0.3 mL ye-alkaline mer curic-potassium iodide TS, i-cap the test tubes, kwaye uvumele ukuma i-5 min.
Indlela yolwamkelo: ISampuli yesisombululo asibonisi umbala omthubi kakhulu kunalowo wesisombululo soMgangatho (NMT 4 ppm yeammonia).
• UMDA WEKHLORIDE
Isisombululo sesampuli: Nxiba i-0.2 g kwi-10 mL yamanzi.Gxuma i-30 s, udlule kwisihluzo esirhabaxa, kwaye usebenzise i-filtrate.
Isisombululo esisemgangathweni: 2.3 µg/mL yesodium chloride emanzini
Uhlalutyo: Kwisisombululo seSampule kunye ne-10 mL yesisombululo esiPhakathi songeza i-1 mL ye-nitric acid kunye ne-1 mL ye-nitrate yesilivere ye-TS, kwaye udibanise isisombululo ngasinye ngamanzi kwi-40 mL.Vumela izisombululo ukuba zime i-5 min, zikhuselwe ekukhanyeni.
Indlela yolwamkelo: Xa ijongwe ngokuchasene nemvelaphi emnyama, isisombululo seSampulu asikho i-turbid kunesisombululo esiseMgangathweni (NMT 0.007% chloride).
• UMDA WESULFATE
Isisombululo sesampuli: Nxiba i-0.75 g kwi-15 mL yamanzi.Gxuma i-30 s, udlule kwisihluzo esirhabaxa, kwaye usebenzise i-filtrate.
Isisombululo esisemgangathweni: Yongeza i-0.15 mL ye-0.020 N sulfuric acid ukuya kwi-15 mL yamanzi.
Uhlalutyo: Kwisisombululo seSampuli kunye nesisombululo esiPhakathi songeza i-2 mL ye-barium chloride TS kunye ne-1 mL ye-3 N hydrochloric acid, xuba isisombululo ngasinye ngamanzi kwi-30 mL, kwaye udibanise.Vumela izisombululo ukuba zime i-5 min.
Indlela yolwamkelo: Isicombululo seSampulu asikho siphithiphithi kunesisombululo esiseMgangathweni (NMT 0.02% sulfate).
• UBUCHUPHEKO BOMDA
Isisombululo A: 6.8 g / L ye-potassium hydrogen sulfate kunye ne-3.4g / L ye-tetrabutylammonium hydrogen sulfate emanzini.Lungisa nge-2 N potassium hydroxide ukuya kwi-pH ye-6.5.
Isisombululo B: 0.1 g / L yesisombululo se-sodium azide
Isigaba esiphathwayo: Isisombululo A kunye nesisombululo B (60:40)
Isisombululo sokufaneleka kwenkqubo: 0.2 mg / mL nganye ye-Adenosine kunye ne-inosine kwisigaba se-Mobile
Isisombululo sesampuli: 1.0 mg / mL ye-Adenosine kwisigaba se-Mobile
Inkqubo yeChromatographic
(Jonga iChromatography <621>, Ukufaneleka kweNkqubo.)
Indlela: LC
Umtshina: UV 254 nm
Ikholomu: 4.6-mm × 25-cm;5-µm ukupakisha i-L1
Izinga lokuhamba: 1.5 mL / min
Ubungakanani betofu: 20 µL
Ukufaneleka kwenkqubo
Iisampulu: Isisombululo sokufaneleka kwenkqubo
Iimfuno zokufaneleka
Isisombululo: NLT 9.0 phakathi kwe-adenosine kunye ne-inosine
Umba wokubala: NMT 2.5
Ukutenxa umgangatho ozalanayo: NMT 2.0%
[QAPHELA-I-Chromatograph isisombululo seSampuli, kwaye ulungelelanise ixesha lokuqhuba ubuncinane kabini kwixesha lokugcinwa kwencopho enkulu.]
Uhlalutyo
Isampulu: Isampuli yesisombululo
Bala ipesenti yokungahlambuluki ngakunye kwisahlulo seAdenosine esithathiweyo:
Isiphumo = (rU/rT) × 100
I-rU = impendulo ephezulu yokungahlambuluki nganye kwisisombululo seSampuli
rT = isimbuku sazo zonke iimpendulo zazo zonke iincopho ezivela kwiSampuli yesisombululo
Iinqobo zokwamkelwa
Ukungcola komntu ngamnye: NMT 0.1% nganye ye-guanosine, inosine, kunye ne-uridine, kunye ne-NMT 0.2% ye-adenine
Ubumdaka bubonke: NMT 0.5%
IIMVAVANYO EZITHILE
• UDIDI OKUnyibilika OKANYE UBUSHUSHU <741>: 233°-238°
• UKUJONGISWA KOMBONISO, ukujikeleziswa okuthe ngqo <781S>: -68° ukuya -72°
Isisombululo sovavanyo: 20 mg / mL kwisisombululo se-sodium hydroxide (1 kwi-20), inqunywe kwisampuli eyomisiwe ngaphambili kwi-105 ° ye-2h
I-ASIDI OKANYE UBULUNGU: Nxiba i-1 g kwi-20 mL yamanzi angenayo i-carbon dioxide.Gxuma i-30 s, kwaye udlule kwisihluzo esirhabaxa.Kwinxalenye nganye ye-10-mL ye-filtrate nganye yongeza i-0.1mL ye-bromocresol purple TS.
Iinqobo zokwamkela: I-NMT 0.3 mL ye-0.01 N sodium hydroxide iyafuneka ukuvelisa umbala oblowu-violet kwindawo enye.I-NMT 0.1 mL ye-0.01 N i-hydrochloric acid iyafuneka ukuvelisa umbala omthubi kwelinye icandelo.
•UKUSOMISA I-LOSSON <731>: Yomisa isampuli kwi-105℃ kangange-2 h: ilahlekelwa yi-NMT nge-0.5% yobunzima bayo.
IIMFUNO EZONGEZELELWEYO
•UKUPAKISHWA NOKUGCINA: Zigcine kwizikhongozeli eziqinile, ezikwaziyo ukumelana nokukhanya, kwaye uzigcine kwindawo elawulwayo yobushushu begumbi.
• IMIGANGATHO YE-USP REFERENCE <11>
USP Adenosine RS